Nano foliar spraying agent for improving high temperature resistance of wheat as well as preparation method and application of nano foliar spraying agent

Abstract

The invention belongs to the technical field of agricultural production, and particularly relates to a nano foliar spraying agent for improving the high temperature resistance of wheat as well as a preparation method and application of the nano foliar spraying agent. The main component of the nano foliar spraying agent is Chinese wolfberry-liquorice-based carbon quantum dots MIX, Chinese wolfberry is used as a raw material, the nano foliar spraying agent is synthesized through a one-step hydrothermal synthesis method, and the nano foliar spraying agent can be used after being simply purified. The used material is common medlar which is cheap and easy to obtain; the synthesis method is simple, and used equipment is common; the nano foliage spraying agent obtained by the method can effectively improve the high temperature resistance of wheat.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural production, and specifically relates to a nano foliar spraying agent for improving the high temperature resistance of wheat, and a preparation method and application thereof. Background Art

[0002] Global warming has become a major challenge restricting global agricultural production, especially in Xinjiang, where extreme high temperatures have posed a severe obstacle to local agricultural development. As an important food crop widely grown in the region, the growth of wheat has attracted much attention. During the wheat planting process, high temperatures will slow down the rate of wheat photosynthesis, leading to a reduction in photosynthetic products. More seriously, high temperatures during the wheat filling period will directly lead to the obstruction of wheat filling, which will have a serious impact on the formation of wheat yields. This impact not only reduces the economic benefits of agricultural planting, but also poses a threat to food security.

[0003] Plant nanobiology, as an emerging interdisciplinary subject, has a unique approach to exploring ways to improve the high temperature tolerance of crops. Although a variety of nanomaterials synthesized from industrial raw materials have been developed to improve the photosynthetic efficiency and heat tolerance of crops at high temperatures, the preparation of these materials is often accompanied by strict purification and safety assessment processes. Therefore, using Chinese medicinal plants as raw materials to synthesize nanomaterials that can improve wheat's high temperature tolerance is of great significance to the development of sustainable agriculture.

[0004] Therefore, exploring and applying an environmentally friendly, simple and efficient raw material to improve wheat's high temperature resistance is one of the most important issues at this stage. Summary of the invention

[0005] The purpose of the present invention is to provide a nano foliar spray for improving the high temperature resistance of wheat, and a preparation method and application thereof. The raw materials of the nano foliar spray provided by the present invention are not only derived from Chinese medicinal plants, but also reduce the dependence on traditional industrial raw materials. In addition, the nano foliar spray can significantly improve the high temperature resistance of wheat by preparing the nano foliar spray.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] On the one hand, the present invention provides a nano foliar spray for improving the high temperature resistance of wheat, wherein the component of the nano foliar spray is wolfberry-licorice-based carbon quantum dot MIX.

[0008] On the other hand, the present invention also provides a method for preparing a nano foliar spray, comprising the following steps:

[0009] The wolfberry and licorice are crushed and placed in a solvent and stirred to obtain a mixture, and then the mixture is placed in a reactor for heating at 160-170°C. After the heating is completed, it is cooled to room temperature, and then the reaction product is centrifuged at 3000-5000rpm. The supernatant is taken to obtain the wolfberry-licorice-based carbon quantum dot MIX, which is stored at 3-5°C for later use.

[0010] Preferably, the nano foliar spray contains a surfactant, and the surfactant is Silwet L-77, which accounts for 0.05% of the volume of the nano material solution.

[0011] Preferably, the nano foliar spray is a nano material solution, and the concentration of the nano material solution is 80-300 mg / L.

[0012] Preferably, the mass ratio of wolfberry to licorice is (1-3):1.

[0013] On the other hand, the present invention also provides an application of a nano foliar spray in improving the high temperature resistance of wheat.

[0014] Preferably, the nano foliar spray is applied when the wheat grows to the one-leaf-one-heart development stage, and is applied to the leaf surface using a pipette.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention uses wolfberry as a raw material and synthesizes a nano foliar spray agent through a one-step hydrothermal synthesis method, which can be used after simple purification. The material used in the present invention is common wolfberry, which is cheap and easy to obtain; the synthesis method is simple, and the equipment used is common; the synthesized nano material can be used after simple centrifugation; the nano material solution obtained by the method can effectively improve the high temperature resistance of wheat. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0018] Figure 1 TEM images of Control and MIX.

[0019] Figure 2 It is the fresh weight of the aboveground part of wheat after 3 days of high temperature stress.

[0020] Figure 3 It is the fresh weight of underground part of wheat after 3 days of high temperature stress.

[0021] Figure 4 SPAD value of wheat under high temperature stress for 3 consecutive days.

[0022] The solution of the Control group is deionized water containing 0.05% Silwet L-77; the solution of the MIX group is a 150 mg / L nanomaterial mixed solution containing 0.05% Silwet L-77. DETAILED DESCRIPTION

[0023] The purpose of the present invention is to provide a nano foliar spray for improving the high temperature resistance of wheat and a preparation method and application thereof.

[0024] In one aspect, the present invention provides a nano foliar spray for improving the high temperature resistance of wheat, wherein the nano foliar spray is a nano material solution, and the concentration of the nano material solution is 80-300 mg / L, preferably 150 mg / L.

[0025] On the other hand, the present invention provides a method for preparing a nano foliar spray, comprising the following steps: grinding wolfberry and licorice, placing them in a solvent and stirring them to obtain a mixture, then placing the mixture in a reactor for heating treatment at 160-170°C, cooling to room temperature after the heating is completed, and then centrifuging the reaction product at 3000-5000rpm, taking the supernatant to obtain wolfberry-licorice-based carbon quantum dot MIX, and storing it at 3-5°C for later use.

[0026] The mass ratio of wolfberry to licorice is (1-3):1. Preferably, it is 1:1; the mixture is heated at 160-170°C; the reaction product is centrifuged at 3000-5000rpm. Preferably, the heating temperature is 160°C and the centrifugation temperature is 3000rpm.

[0027] On the other hand, the present invention provides an application of a nano foliar spray in improving the high temperature resistance of wheat.

[0028] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0029] The production processes, experimental methods or detection methods involved in the embodiments of the present invention, unless otherwise specified, are all conventional methods in the prior art, and their names and / or abbreviations are all conventional names in the field, and are very clear and unambiguous in the relevant application fields. Technical personnel in the field can understand the conventional process steps based on the names and apply the corresponding equipment, and implement them according to conventional conditions or the conditions recommended by the manufacturer.

[0030] The various instruments, equipment, raw materials or reagents used in the embodiments of the present invention are not particularly limited in terms of their sources, and are all conventional products that can be purchased through regular commercial channels, or can be prepared according to conventional methods well known to those skilled in the art.

[0031] Example 1

[0032] Crush wolfberry and licorice, weigh 200 mg wolfberry powder and 200 mg licorice powder, mix well, add 20 mL distilled water, put into a 50 mL polytetrafluoroethylene reactor, the reaction temperature is 160 ° C, the reaction time is 6 hours. After cooling, take out and pack into 1.5 mL centrifuge tubes, centrifuge at 3000 rpm for 1 hour, take the supernatant, and get a brown liquid with a sweet aroma, which is wolfberry-licorice-based carbon quantum dots MIX LWCMs. LWCMs are finally stored in a 4 ° C refrigerator for subsequent use.

[0033] Example 2

[0034] Example 2 is substantially the same as Example 1, except that the weight of wolfberry and liquorice powder is 300 mg each, the heating temperature is 170° C., and the centrifugal speed is 5000 rpm.

[0035] Example 3

[0036] Example 3 is substantially the same as Example 1, except that the weight of wolfberry and liquorice powder is 500 mg each, the heating temperature is 160° C., and the centrifugal speed is 3000 rpm.

[0037] Example 4

[0038] Based on the LWCMs prepared in Example 1, the following operations were continued: wheat seeds with full grains, uniform size and no damage were selected, and the surface was disinfected with 75% medical alcohol for 30 seconds, and then rinsed with deionized water for 4-6 times, and the disinfected seeds were placed at room temperature to dry for use. The disinfected seeds were sown into seed germination bags, 5 seeds were sown in each germination bag, and 15 mL of distilled water was added.

[0039] Example 5

[0040] The wheat was treated when it reached the one-leaf-one-heart stage. Two different treatment solutions were prepared: one was deionized water containing 0.05% SilwetL-77 as a control; the other was a 150mg / L nanomaterial mixed solution containing 0.05% SilwetL-77 as an experiment. Then, the tip of the 1000μl gun tip was reduced by 0.3cm to avoid mechanical damage to the leaves by the sharp gun tip, and a 1mL pipette was used to evenly apply the solution on the leaf surface. After applying, use a paper towel to absorb the excess solution on the leaf surface. The treated wheat and the control group wheat were placed in a weak light place for 3 hours to adapt, and then placed in a 39℃ incubator for high temperature stress.

[0041] Example 6

[0042] To evaluate the effect of spraying a mixed solution containing 0.05% Silwet L-77 surfactant and 150 mg / L nanomaterials on the chlorophyll content of wheat seedlings under three consecutive days of high temperature stress, the SPAD values ​​of wheat seedlings treated with 150 mg / L mixed solution containing 0.05% Silwet L-77 and deionized water containing 0.05% Silwet L-77 were measured on the 1st, 2nd, and 3rd days of high temperature stress. The SPAD value of the first true leaf was measured 5 times for each leaf and the average value was taken ( Figure 4 ).

[0043] Example 7

[0044] After 3 days of stress, the wheat seedlings were taken out, photographed and weighed. The wheat seedlings treated with the nanomaterial solution were significantly larger than those treated with 0.05% Silwet L-77. Figure 1 As shown, and has a higher aboveground part as Figure 2 As shown and underground fresh weight as Figure 3 shown.

[0045] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A nano foliar spray for improving the high temperature resistance of wheat, characterized in that: The component of the nano foliar spray is wolfberry-licorice-based carbon quantum dot MIX.

2. The nano foliar spray for improving the high temperature resistance of wheat according to claim 1, characterized in that: The preparation method of the nano foliar spray comprises the following steps: The wolfberry and licorice are crushed and placed in a solvent and stirred to obtain a mixture, and then the mixture is placed in a reactor for heating at 160-170°C. After the heating is completed, it is cooled to room temperature, and then the reaction product is centrifuged at 3000-5000rpm. The supernatant is taken to obtain the wolfberry-licorice-based carbon quantum dot MIX, which is stored at 3-5°C for later use.

3. The nano foliar spray according to claim 1, characterized in that: The nano foliar spray contains a surfactant, which is Silwet L-77, accounting for 0.05% of the volume of the nano material solution.

4. The nano foliar spray according to claim 1, characterized in that: The nano foliar spray is a nano material solution, and the concentration of the nano material solution is 80-300 mg / L.

5. The method for preparing the nano foliar spray according to claim 2, characterized in that: The mass ratio of wolfberry to licorice is (1-3):

1.

6. Application of a nano foliar spray in improving wheat's resistance to high temperatures.

7. The use according to claim 6, characterized in that: The nano foliar spray is sprayed when the wheat grows to the stage of one leaf and one heart development, and is applied to the leaf surface using a pipette gun.

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