De-ester Yarrowia liplytica CGMCC No.0692 and its use in petroleum refinement

Abstract

A Yarrowia lipolytica (GMCC No.0692) and its application in refining petroleum are disclosed. Said application process includes such steps as slant culturing at 25-30 deg.C, shake culturing, fermenting respectively and sequentially in stage-one and stage-three seeding tanks for 10-18 hr, mixing with raw oil, water and culture medium, fermenting in production tank for 30-100 hr, heating and emulsifying. Its advantages are high activity, dewaxing efficiency and productivity, easy demulsifying, and high quality of oil product.

Description

technical field

[0001] The invention relates to the technique of refining petroleum by using microbial fermentation, and is a strain of Yarrowia esterolytica and its application method in petroleum refining. Background technique

[0002] In 1906, the technology of microbial oil refining was reported, mainly for the microbial oxidation of petroleum gas and methane, and the oxidation mechanism of low-level petroleum hydrocarbons and other basic researches were carried out. By 1963, France reported the oxidation method of petroleum hydrocarbons, and then other countries successively carried out petroleum fermentation research and obtained industrialization results. At present, low-point lubricating oil products and base oil products can be produced by using microbial methods. Since microbial fermentation is generally carried out at normal temperature and pressure, the high temperature and high pressure conditions required for the production of petrochemical products by conventi...

Examples

Embodiment 1

[0038] Embodiment one: according to the attached drawing figure 1 Process operation to obtain 45# transformer oil base oil.

[0039] In a sterile room, transfer the yeast of the present invention to a small shake flask, a large shake flask, a first-stage tank, and a third-stage tank for five-stage culture and proliferation, and connect it to a fermenter containing raw material oil. After 30 hours of fermentation, take a sample to measure the freezing point Qualified, stop the tank, demulsify, settle, and centrifuge to obtain low freezing point 45# transformer oil base oil and petroleum yeast waste liquid.

Embodiment 2

[0041] According to the attached picture figure 2 Process flow operation to obtain 22# refrigerating machine oil base oil.

[0042] In the sterile room, the yeast was transferred to small shake flasks, large shake flasks, first-level tanks, and third-level tanks for five-level culture and multiplication, and then connected to the fermentation tank containing raw material oil. After 100 hours of fermentation, sampling and measuring pour point passed , stop the tank, demulsify, settle, and centrifuge to obtain low freezing point 22# refrigerating machine oil base oil and petroleum yeast waste liquid.

Embodiment 3

[0044] According to the attached picture image 3 Process flow operation to obtain 32# refrigerating machine oil base oil.

[0045] In the sterile room, the yeast was transferred to small shake flasks, large shake flasks, first-level tanks, and third-level tanks for five-level culture and proliferation, and then connected to a fermentation tank containing raw material oil. After 60 hours of fermentation, sampling and measuring pour point passed , stop the tank, break the emulsion, settle, and centrifuge to obtain low freezing point 32# refrigerating machine oil base oil and petroleum yeast waste liquid.

technical field

[0001] The invention relates to the technique of refining petroleum by using microbial fermentation, and is a strain of Yarrowia esterolytica and its application method in petroleum refining. Background technique

[0002] In 1906, the technology of microbial oil refining was reported, mainly for the microbial oxidation of petroleum gas and methane, and the oxidation mechanism of low-level petroleum hydrocarbons and other basic researches were carried out. By 1963, France reported the oxidation method of petroleum hydrocarbons, and then other countries successively carried out petroleum fermentation research and obtained industrialization results. At present, low-point lubricating oil products and base oil products can be produced by using microbial methods. Since microbial fermentation is generally carried out at normal temperature and pressure, the high temperature and high pressure conditions required for the production of petrochemical products by conventi...

Examples

Embodiment 1

[0038] Embodiment one: according to the attached drawing figure 1 Process operation to obtain 45# transformer oil base oil.

[0039] In a sterile room, transfer the yeast of the present invention to a small shake flask, a large shake flask, a first-stage tank, and a third-stage tank for five-stage culture and proliferation, and connect it to a fermenter containing raw material oil. After 30 hours of fermentation, take a sample to measure the freezing point Qualified, stop the tank, demulsify, settle, and centrifuge to obtain low freezing point 45# transformer oil base oil and petroleum yeast waste liquid.

Embodiment 2

[0041] According to the attached picture figure 2 Process flow operation to obtain 22# refrigerating machine oil base oil.

[0042] In the sterile room, the yeast was transferred to small shake flasks, large shake flasks, first-level tanks, and third-level tanks for five-level culture and multiplication, and then connected to the fermentation tank containing raw material oil. After 100 hours of fermentation, sampling and measuring pour point passed , stop the tank, demulsify, settle, and centrifuge to obtain low freezing point 22# refrigerating machine oil base oil and petroleum yeast waste liquid.

Embodiment 3

[0044] According to the attached picture image 3 Process flow operation to obtain 32# refrigerating machine oil base oil.

[0045] In the sterile room, the yeast was transferred to small shake flasks, large shake flasks, first-level tanks, and third-level tanks for five-level culture and proliferation, and then connected to a fermentation tank containing raw material oil. After 60 hours of fermentation, sampling and measuring pour point passed , stop the tank, break the emulsion, settle, and centrifuge to obtain low freezing point 32# refrigerating machine oil base oil and petroleum yeast waste liquid.

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