Seed culture tank for producing beta-cyclodextrin

Abstract

The utility model relates to a seed culture tank for producing beta-cyclodextrin, which belongs to the field of biological fermentation equipment and comprises a culture tank body, an air injection pipe for injecting sterile air into the culture tank body, a feeding pipe for injecting a culture solution into the tank, an exhaust pipe for exhausting redundant gas in the tank, a discharge pipe and a temperature control device, an outlet of the exhaust pipe is connected with a gas-liquid separator, and the device is characterized by further comprising a gas supplementing pipe, an inlet of the gas supplementing pipe is connected with the gas-liquid separator through a flow divider, an inlet of the flow divider is connected with a gas outlet of the gas-liquid separator, one outlet of the flow divider is connected with the outlet end of the gas supplementing pipe, and the other outlet of the flow divider serves as a waste discharging port. An outlet of the air supply pipe extends into the culture tank body and is close to the bottom wall of the culture tank body; according to the utility model, through the additional arrangement of the blowdown pipe, the unconsumed oxygen contained in the blowdown pipe can be reused, the tail gas treatment pressure is reduced, a certain effervescence effect can be achieved, and the cost of fresh air is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological fermentation equipment, in particular to a seed culture tank for producing beta-cyclodextrin. Background Art

[0002] Cyclodextrin (CD) is a general term for a series of cyclic oligosaccharides generated by amylose under the action of cyclodextrin glucosyltransferase produced by Bacillus. Cyclodextrin containing seven glucose units is called β-cyclodextrin, and each glucose unit is bonded into a slightly conical ring by 1,4-glycosidic bonds. At present, β-cyclodextrin (β-CD) and its derivatives are a common and efficient supramolecular inclusion material, which is widely used in the separation and synthesis of organic compounds, and is also used as a pharmaceutical excipient, food additive, etc.

[0003] The production process of β-cyclodextrin generally includes hydrolysis, extraction, decolorization, filtration, purification, drying, crushing and other processes, wherein the hydrolysis process generally uses starch as a substrate and uses an enzyme solution for hydrolysis, and the enzyme solution used in the industrial production of β-cyclodextrin is mostly cyclodextrin glucosyltransferase produced by fermentation of Bacillus softening and Bacillus alkaliphilus. The production process of the enzyme solution generally includes slant strains, primary seed culture, secondary seed culture, fermentation and other processes, wherein the secondary seed culture is generally completed in a seed culture tank.

[0004] The seed culture tank in the prior art is shown in the patent document with the announcement number CN215209390U, which includes a culture tank body, an air injection pipe fixed on the culture tank body for injecting air into the culture tank body, an air exhaust pipe fixed on the upper end of the culture tank body, a material injection pipe for injecting culture liquid into the culture tank body, a discharge pipe and a temperature control device; an air heater for heating air, an air filter for filtering dust in the air, a sterilizer for eliminating fungi in the air and an air pump for generating air pressure in the air are arranged on the air injection pipe; and a gas-liquid separator for separating moisture and gas in the air is fixed on the air exhaust pipe.

[0005] The prior art such as the above structure can provide the oxygen required for the secondary seed culture, but a part of the unconsumed oxygen will be discharged through the air exhaust pipe, resulting in an increase in the exhaust gas treatment amount. Utility Model Content

[0006] The technical problem to be solved by the utility model is to provide a seed culture tank for producing beta-cyclodextrin in view of the deficiencies of the prior art.

[0007] The utility model provides a technical solution to the above-mentioned technical problems as follows: a seed culture tank for producing β-cyclodextrin, comprising a culture tank body, an air injection pipe for injecting sterile air into the culture tank body, a feeding pipe for injecting culture liquid into the tank, an exhaust pipe for discharging excess gas in the tank, a discharge pipe and a temperature control device, the outlet of the exhaust pipe being connected with a gas-liquid separator, characterized in that it also comprises an air supply pipe, the inlet of the air supply pipe being connected with the gas-liquid separator through a diverter, the inlet of the diverter being connected with the gas outlet of the gas-liquid separator, one outlet of the diverter being connected with the outlet end of the air supply pipe, the other outlet of the diverter being used as a waste outlet, and the outlet of the air supply pipe extending into the culture tank body, close to the bottom wall of the culture tank body.

[0008] Furthermore, the culture tank body is a tank body structure with a jacket inside, and the culture tank body is connected to a cold / heat medium circulation device communicated with the inner cavity of the jacket.

[0009] Furthermore, the air supply pipe is connected to an air pump for providing power for the gas to flow from the diverter to the outlet of the air supply pipe.

[0010] Furthermore, an air pump is connected to the air injection pipe to serve as a power source for injecting sterile air into the tank through the air injection pipe.

[0011] Furthermore, the flow splitter is a three-way flow splitter with one inlet and two outlets.

[0012] Furthermore, two outlets of the diverter are independently provided with control valves.

[0013] The beneficial effect of the utility model is that the utility model adds an air supply pipe on the basis of the existing technology, the air intake of the air supply pipe is connected to the exhaust pipe of the tank body through a diverter, and the air outlet of the air supply pipe is connected to the bottom of the tank. Not only can the unconsumed oxygen contained in the air supply pipe be reused to reduce the pressure of exhaust gas treatment, but also it can play a certain effervescent effect and reduce the cost of filtering and sterilizing the new air. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0015] Figure 2 It is a schematic diagram of the top view of the aeration plate of the utility model;

[0016] In the figure: 1. culture tank, 2. air injection pipe, 21. aeration plate, 3. feeding pipe, 4. exhaust pipe, 5. discharge pipe, 6. gas-liquid separator, 7. air supply pipe, 71. air supply plate, 8. diverter, 9. air pump, 10. control valve, 11. exhaust port, 12. air hole. DETAILED DESCRIPTION

[0017] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The embodiments described are only used to explain the present invention and are not used to limit the scope of the present invention.

[0018] As shown in the accompanying drawings, the seed culture tank for producing β-cyclodextrin in this embodiment includes a culture tank body 1, an air injection pipe 2 for injecting sterile air into the culture tank body 1, a feeding pipe 3 for injecting culture liquid into the tank, an exhaust pipe 4 for discharging excess gas in the tank, and a discharge pipe 5 and a temperature control device; the exhaust pipe 4 is connected to the top of the culture tank body 1, and its outlet is connected to a gas-liquid separator 6 for gas-liquid separation of the discharged gas-liquid mixture; the culture tank body 1 has a jacket inside, and the culture tank body 1 is connected to a cold / hot medium circulation device connected to the inner cavity of the jacket, and the cold / hot medium circulation device is electrically connected to the temperature control device, and the temperature of the material in the tank is controlled by introducing cold / hot medium into the jacket of the culture tank body 1 and forming a circulation; the air injection pipe 2 is connected to an air filtration system and a sterilization system, which are all existing technologies and will not be repeated here. The purpose is to provide clean and sterile air to the culture tank body 1.

[0019] The seed culture tank also includes an air supply pipe 7, the inlet of which is connected to the gas outlet of the gas-liquid separator 6 through a diverter 8. The diverter 8 of this embodiment is a three-way diverter with one inlet and two outlets. The inlet of the diverter 8 is connected to the outlet of the gas-liquid separator 6, one outlet of the diverter 8 is connected to the outlet end of the air supply pipe 7, and the other outlet of the diverter 8 is used as an exhaust gas outlet 11. The outlet of the air supply pipe 7 extends into the culture tank body 1, close to the bottom wall of the culture tank body 1. The air supply pipe 7 is connected to an air pump 9 for providing power for the gas to flow from the diverter 8 to the outlet of the air supply pipe 7; the air injection pipe 2 is connected to an air pump 9 as a power source for injecting sterile air into the tank through the air injection pipe 2, and an independent switch valve is provided on the air injection pipe 2.

[0020] In this embodiment, the bottom of the culture tank body 1 has an aeration plate 21 connected to the gas injection pipe 2, which has a C-shaped structure when viewed from above, and has air holes 12 on its surface. The gas injection pipe 2 is connected to its side edge and connected to the air holes 12; the bottom of the culture tank body 1 has an air supply plate 71 connected to the air supply pipe 7, which has a disc-shaped structure when viewed from above and is embedded in the core of the aeration plate 21, and has air holes 12 on its surface. The air supply pipe 7 is connected to it and leads out through the notch of the aeration plate 21. This solution can ensure that the aeration plate 21 and the air supply plate 71 are both located at the bottom of the tank, ensuring the effervescent effect of the two.

[0021] During operation, materials such as bacterial strains and culture media are added to the culture tank body 1 through the feeding pipe 3, and sterilized air is introduced into the bottom of the tank through the air injection pipe 2 to provide oxygen for the aerobic bacteria in the tank, and the temperature in the tank is controlled by the temperature control device. As the seed culture proceeds, the gas-liquid mixture produced is separated into gas and liquid by the gas-liquid separator 6, and the separated gas enters the diverter 8. The two outlets of the diverter 8 are independently provided with control valves 10. By adjusting the ventilation flow of the two outlet control valves 10 of the diverter 8, a part of the gas enters the air supply pipe 7 while ensuring that the pressure in the tank is within a safe range, and the rest of the gas is discharged through the exhaust port, and can enter the exhaust gas treatment system according to the actual situation. The gas in the air supply pipe 7 re-enters the culture tank body 1 and the bottom of the material under the action of the air pump. On the one hand, the unconsumed oxygen contained therein can be reused to reduce the pressure of exhaust gas treatment. On the other hand, it can play a certain effervescent effect, which can reduce the consumption of newly treated sterile air from the air injection pipe 2, that is, reduce the cost of air filtration and sterilization treatment. In addition, a mechanical stirring mechanism is often configured in a general seed tank. The effervescent effect generated by the setting of the air supply pipe can reduce the speed of mechanical stirring to a certain extent (under the premise that the air injection volume remains unchanged), thereby reducing the shear force of mechanical stirring on the material and avoiding the negative impact of excessive shear force on the material.

Claims

1. A seed culture tank for producing β-cyclodextrin, comprising a culture tank body (1), an air injection pipe (2) for injecting sterile air into the culture tank body (1), a feeding pipe (3) for injecting culture liquid into the tank, an exhaust pipe (4) for discharging excess gas in the tank, a discharge pipe (5) and a temperature control device, wherein the outlet of the exhaust pipe (4) is connected to a gas-liquid separator (6), characterized in that: The invention also comprises an air supply pipe (7), the inlet of which is connected to the gas-liquid separator (6) via a diverter (8), the inlet of which is connected to the gas outlet of the gas-liquid separator (6), one outlet of the diverter (8) is connected to the outlet end of the air supply pipe (7), the other outlet of the diverter (8) serves as a waste outlet, and the outlet of the air supply pipe (7) extends into the culture tank body (1) and is close to the bottom wall of the culture tank body (1).

2. The seed culture tank for producing β-cyclodextrin according to claim 1, characterized in that: The culture tank body (1) is a tank body structure having a jacket inside, and the culture tank body (1) is connected to a cold / heat medium circulation device which is in communication with the inner cavity of the jacket.

3. The seed culture tank for producing β-cyclodextrin according to claim 1, characterized in that: The air supply pipe (7) is connected to an air pump (9) for providing power for the gas to flow from the diverter (8) toward the outlet of the air supply pipe (7).

4. The seed culture tank for producing β-cyclodextrin according to claim 1, characterized in that: The air injection pipe (2) is connected to an air pump (9) serving as a power source for injecting sterile air into the tank through the air injection pipe (2).

5. The seed culture tank for producing β-cyclodextrin according to claim 1, characterized in that: The flow splitter (8) is a three-way flow splitter (8) of one-inlet and two-outlet type.

6. The seed culture tank for producing β-cyclodextrin according to claim 5, characterized in that: The two outlets of the flow divider (8) are independently provided with control valves (10).

7. The seed culture tank for producing β-cyclodextrin according to claim 1, characterized in that: The inner bottom of the culture tank body (1) is provided with an aeration plate (21) connected to the air injection pipe (2) and an air supply plate (71) connected to the air supply pipe (7).

8. The seed culture tank for producing β-cyclodextrin according to claim 7, characterized in that: The aeration plate (21) and the air supply plate (71) are in a nested structure.

9. The seed culture tank for producing β-cyclodextrin according to claim 8, characterized in that: The aeration plate (21) is in a C-shaped structure, and the air supply plate (71) is in a disc-shaped structure.

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