Stem cell culture vessel

Abstract

The utility model relates to the field of culture dishes, in particular to a stem cell culture dish which comprises a base body and a base cover which are in threaded connection, a focusing groove is downwards formed in the center of the inner bottom of the base body, the base body is provided with an observation base relative to the outer bottom of the focusing groove, and the observation base is arranged in a protrusion shape extending downwards. The thickness of the observation base is far smaller than that of the base body, a limiting ring extending downwards is arranged on the periphery of the outer bottom of the base body, a positioning ring is arranged on the top of the base cover, and the base body is inserted into the inner periphery of the positioning ring through the limiting ring at the bottom, so that the adjacent culture vessels are stacked up and down; the system also comprises an RFID tag. The RFID tag is detachably arranged on the side wall of the base cover, and the RFID tag is in an arc shape and is attached to the periphery of the base cover. The utility model is used for solving the problem that cells are inconvenient to observe from the bottom due to the thick bottom of the culture dish in the prior art, and also provides a structure in which multiple groups of culture dishes are conveniently and stably stacked and are convenient to identify.

Description

Technical Field

[0001] The utility model relates to the technical field of culture dishes, in particular to a stem cell culture vessel. Background Art

[0002] Stem cells are a type of cell with unlimited or immortal self-renewal capabilities. Stem cells are of great significance in basic research and medical applications. Stem cell culture vessels are required for stem cell culture. Traditional stem cell culture vessels generally include a base and a base cover, which are connected by threads to culture stem cells.

[0003] During the culture process, an inverted microscope is generally used, and the lens needs to pass through the bottom of the culture dish from below to see the living cells on the culture dish clearly. The thickness of the bottom of a general cell culture dish is thick and cannot meet this observation. Moreover, multiple groups of culture dishes need to be set up during culture. The upper and lower ends of conventional culture dishes are flat in design, and the stability is not high when stacked. In addition, in order to avoid confusion, each culture dish needs to be numbered. The traditional method of handwritten marking is used, but it is easy to make mistakes due to the ambiguity and poor recognition of the mark. Moreover, when there is a lot of information to be marked, it is difficult for manual marking to express the information comprehensively and completely.

[0004] Therefore, those skilled in the art provide a stem cell culture vessel to solve the problems raised in the above background technology. Utility Model Content

[0005] In order to solve the above technical problems, the utility model provides a stem cell culture vessel, which is used to solve the problem in the prior art that the bottom of the culture vessel is thick and it is inconvenient to observe the cells from the bottom. It also provides a structure for multiple groups of culture vessels to be stably stacked and easy to mark.

[0006] The technical solution is as follows:

[0007] It comprises a base body and a base cover, wherein the inner wall of the base cover is provided with an internal thread, the outer wall of the base body is provided with an external thread, and the base cover is threadedly connected to the outer top of the base body through the internal thread;

[0008] A focusing groove is provided downward at the center of the inner bottom of the substrate, an observation base is provided at the outer bottom of the substrate relative to the focusing groove, the observation base is provided in a convex shape extending downward, the thickness of the observation base is much smaller than the thickness of the substrate, a limiting ring extending downward is provided at the outer periphery of the outer bottom of the substrate, the limiting ring is located at the outer periphery of the observation base, and the bottom of the limiting ring is provided below the horizontal plane of the bottom of the observation base;

[0009] The top of the base cover is connected with a positioning ring, and the base body is inserted into the inner circumference of the positioning ring through the limiting ring at the bottom, so that adjacent culture vessels can be stacked up and down; the base body is arranged in an inverted truncated cone shape, and the base cover is arranged in a truncated cone shape;

[0010] It also includes an RFID tag; the RFID tag is detachably arranged on the side wall of the base cover, and the RFID tag is arc-shaped and is arranged to fit the outer periphery of the base cover.

[0011] Preferably, it also includes a partition assembly, which is composed of multiple groups of radial partition plates, the inner wall of the substrate is provided with a slot, and multiple groups of the slots are arranged in an array along the inner periphery of the substrate, and the outer ends of the partition plates are clamped in the slots, and different partition chambers are formed between the partition plates, which is convenient for different experimental treatments. Under the orthogonal experiment, batch culture is carried out under the same conditions and different drug concentrations.

[0012] Preferably, a medical sterile silicone pad is provided at the bottom of the partition plate, and the silicone pad is in close contact with the inner bottom wall of the substrate, so that a sealed space is formed between the partition plate and the bottom wall of the substrate to prevent contamination of adjacent partition compartments.

[0013] Preferably, the focusing groove is a circular through groove, and the observation base is designed as a rectangular sheet.

[0014] Preferably: a mounting groove is provided on the side wall of the base cover, the mounting groove is designed as a groove body with an open top and a hollow interior, the RFID tag is arranged in the mounting groove, and the RFID tag is an RFID flexible tag to realize the function of electronic marking.

[0015] Technical effects and advantages of the utility model:

[0016] The bottom of the base is provided with a focusing groove, an observation base, and a limiting ring in sequence from the inside to the outside; the design of the focusing groove and the observation base is used to solve the problem in the prior art that the bottom of the culture dish is thick and it is inconvenient to observe cells from the bottom by providing a thin sheet-shaped observation base;

[0017] By setting the limiting ring and the positioning ring, a structure is provided for multiple groups of culture dishes to be easily and stably stacked, which is convenient for upper and lower positioning and stacking, saves occupied space, and improves the stability of placement;

[0018] The RFID tag adopts a flexible tag, which is easy to fit the curvature of the base cover, easy to place, and the setting of the tag is convenient for identification;

[0019] The provision of the separation component provides a separation chamber which is convenient for carrying out different test treatments and is convenient for batch culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1This is a schematic diagram of the overall structure of a stem cell culture vessel provided in an embodiment of the present application;

[0021] Figure 2 This is a schematic diagram of the exploded structure of a stem cell culture vessel provided in an embodiment of the present application;

[0022] Figure 3 This is a schematic diagram of the internal structure of a matrix in a stem cell culture vessel provided in an embodiment of the present application;

[0023] Figure 4 This is a schematic diagram of the bottom structure of a substrate in a stem cell culture vessel provided in an embodiment of the present application;

[0024] Figure 5 This is a schematic diagram of the internal structure of a base cover in a stem cell culture vessel provided in an embodiment of the present application;

[0025] In the figure:

[0026] 1. substrate, 11. external thread, 12. focusing groove, 13. observation base, 14. limiting ring;

[0027] 2. base cover, 21. internal thread, 22. positioning ring;

[0028] 3. RFID tag, 31. mounting slot;

[0029] 4. Partition assembly, 41. Partition plate, 42. Card slot, 43. Silicone pad. DETAILED DESCRIPTION

[0030] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present invention are provided for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific purposes.

[0031] Unless otherwise defined, the directions of up, down, left, right, front, back, inside and outside involved in this document are based on the directions of up, down, left, right, front, back, inside and outside in the figures shown in the present utility model, and are explained here together.

[0032] The connection method can be bonding, welding, bolt connection, etc., depending on actual needs.

[0033] Example 1

[0034] See also Figures 1 to 5In this embodiment, a stem cell culture vessel is provided, including a base 1 and a base cover 2. The inner wall of the base cover 2 is provided with an internal thread 11, and the outer wall of the base 1 is provided with an external thread 21. The base cover 2 is threadedly connected to the outer top of the base 1 through the internal thread 11.

[0035] refer to Figure 2 and Figure 3 As shown, a focusing groove 12 is opened downward at the center of the inner bottom of the substrate 1, and an observation base 13 is provided at the outer bottom of the substrate 1 relative to the focusing groove 12. The observation base 13 is arranged in a convex shape extending downward, and the thickness of the observation base 13 is much smaller than the thickness of the substrate 1. A limiting ring 14 extending downward is provided at the outer periphery of the outer bottom of the substrate 1. The limiting ring 14 is located at the outer periphery of the observation base 13, and the bottom of the limiting ring 14 is arranged below the horizontal plane of the bottom of the observation base 13.

[0036] The focusing groove 12 is a circular through groove, and the observation base 13 is a rectangular sheet design.

[0037] The principle of use of this part of the structure is as follows: the bottom of the base 1 is sequentially provided with a focusing groove 12, an observation base 13, and a limiting ring 14 from the inside to the outside. The design of the focusing groove 12 and the observation base 13 is used to solve the problem that the bottom of the culture dish is thick and it is inconvenient to observe cells from the bottom in the prior art by providing a thin sheet-like observation base 13. When it is necessary to observe the state of stem cells, the stem cells are scribed at the top of the focusing groove 12, and an inverted microscope is used to pass the lens through the bottom of the observation base 13 from below to see the living cells on the culture dish. The thickness of the observation base 13 is much smaller than that of the base 1, which is also convenient for observation.

[0038] In order to solve the problem in the prior art that the culture dishes are designed to be flat on both the top and the bottom and have low stability when stacked, a positioning ring 22 is connected to the top of the base cover 2, and the base 1 is inserted into the inner circumference of the positioning ring 22 through the limiting ring 14 at the bottom to achieve the upper and lower stacking of adjacent culture vessels; wherein the base 1 is arranged in an inverted truncated cone shape, and the base cover 2 is arranged in a truncated cone shape.

[0039] When stacked up and down, it saves space and improves placement stability.

[0040] The present solution further includes an RFID tag 3 ; the RFID tag 3 is detachably disposed on the side wall of the base cover 2 , and the RFID tag 3 is arc-shaped and is disposed in close contact with the outer periphery of the base cover 2 .

[0041] In addition, a mounting groove 31 is provided on the side wall of the base cover 2. The mounting groove 31 is designed as a groove body with an open top and a hollow interior. The RFID tag 3 is arranged in the mounting groove 31. The RFID tag 3 is an RFID flexible tag, which realizes the function of electronic marking, is convenient to fit the curvature of the base cover, and is convenient to place. The setting of the tag is convenient for identification, and the content of the electronic tag can be edited.

[0042] The present solution also includes a partition assembly 4, which is composed of multiple groups of radially arranged partition plates 41. The inner wall of the substrate 1 is provided with a card slot 42, and multiple groups of card slots 42 are arranged in an array along the inner periphery of the substrate 1. The outer ends of the partition plates 41 are clamped in the card slots 42, and different partition chambers are formed between the partition plates 41, which is convenient for different experimental treatments. Under the orthogonal experiment, batch culture is performed under the same conditions and different drug concentrations.

[0043] Among them, a medical sterile silicone pad 43 is provided at the bottom of the partition plate 41, and the silicone pad 43 is in close contact with the inner bottom wall of the base 1, so that a sealed space is formed between the partition plate 41 and the bottom wall of the base 1 to prevent contamination of adjacent partition chambers. It can be installed by yourself as needed when in use.

[0044] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without creative work should fall within the scope of protection of the present invention. Structures, devices and operating methods not specifically described and explained in the present invention shall be implemented according to conventional means in the field unless otherwise specified and limited.

Claims

1. A stem cell culture vessel, comprising a base (1) and a base cover (2), wherein the inner wall of the base cover (2) is provided with an internal thread (11), the outer wall of the base (1) is provided with an external thread (21), and the base cover (2) is threadedly connected to the outer top of the base (1) through the internal thread (11), It is characterized in that A focusing groove (12) is provided downwardly at the center of the inner bottom of the base (1); an observation base (13) is provided at the outer bottom of the base (1) relative to the focusing groove (12); the observation base (13) is arranged in a convex shape extending downwardly; and the thickness of the observation base (13) is less than the thickness of the base (1); A limiting ring (14) extending downward is provided on the outer periphery of the outer bottom of the base (1), the limiting ring (14) is located on the outer periphery of the observation base (13), and the bottom of the limiting ring (14) is arranged below the horizontal plane of the bottom of the observation base (13); a positioning ring (22) is connected to the top of the base cover (2), and the base (1) is inserted into the inner periphery of the positioning ring (22) through the limiting ring (14) at the bottom; It also comprises an RFID tag (3); the RFID tag (3) is detachably arranged on the side wall of the base cover (2); the RFID tag (3) is arc-shaped and is arranged to fit the outer periphery of the base cover (2).

2. A stem cell culture vessel according to claim 1, It is characterized in that The invention also comprises a partition assembly (4), wherein the partition assembly (4) is composed of a plurality of groups of partition plates (41) arranged in a radial shape, the inner wall of the base (1) is provided with a card slot (42), a plurality of groups of the card slots (42) are arranged in an array distributed along the inner periphery of the base (1), the outer ends of the partition plates (41) are clamped in the card slots (42), and different partition compartments are formed between adjacent partition plates (41).

3. A stem cell culture vessel according to claim 2, It is characterized in that A silica gel pad (43) is provided at the bottom of the partition plate (41), and the silica gel pad (43) is in close contact with the inner bottom wall of the base (1).

4. The stem cell culture vessel according to claim 1, It is characterized in that The focusing groove (12) is a circular through groove, and the observation base (13) is designed as a rectangular sheet.

5. The stem cell culture vessel according to claim 1, It is characterized in that The side wall of the base cover (2) is provided with a mounting groove (31), the mounting groove (31) is designed as a groove body with an open top and a hollow interior, and the RFID tag (3) is arranged in the mounting groove (31).

6. A stem cell culture vessel according to claim 5, It is characterized in that The RFID tag (3) is an RFID flexible tag.

Images