Solid-phase extraction column and extraction device

Abstract

The utility model discloses a solid-phase extraction column and an extraction device. The volume of the solid-phase extraction column is less than 500 microliters. According to the solid-phase extraction column, the volume of the solid-phase extraction column is set to be less than 500 microliters, so that a protein sample and a reaction solution can be ensured to fully react in the solid-phase extraction column when the solid-phase extraction column is used for testing the small-mass protein sample and the reaction solution, waste is reduced, and the cost is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of protein sample pretreatment, in particular to a solid phase extraction column and an extraction device. Background Art

[0002] Proteomics is a science that studies the protein composition and its changing patterns of cells, tissues or organisms, taking the proteome as the research object. During the research of protein samples, protein samples and reaction solutions can be filled into solid phase extraction columns (SPE). After the protein sample reaction is completed, the reaction solution needs to be extracted to obtain peptide samples that can be analyzed by mass spectrometer.

[0003] In the past, due to the low sensitivity of mass spectrometry detection, a larger volume of solid phase extraction column was needed to fill a larger mass of protein samples and reaction solutions. However, with the improvement of the sensitivity of mass spectrometry detection, it is no longer necessary to use large mass of protein samples and reaction solutions. If the original solid phase extraction column is still used, the volume of the solid phase extraction column is relatively large, causing the protein sample and reaction solution to partially adhere to the inner wall of the solid phase extraction column. At the same time, a large amount of protein sample and reaction solution is required for reaction, and the processing cost is high. Utility Model Content

[0004] The embodiment of the utility model provides a solid phase extraction column and an extraction device to solve at least one of the above-mentioned technical problems.

[0005] The embodiment of the utility model provides a solid phase extraction column and an extraction device. The volume of the solid phase extraction column is less than 500 μL.

[0006] The solid phase extraction column, by setting the volume of the solid phase extraction column to be less than 500 μL, can ensure that the protein sample and the reaction solution fully react in the solid phase extraction column for experiments with small-mass protein samples and reaction solutions, thereby reducing waste and lowering costs.

[0007] In certain embodiments, the solid phase extraction column comprises a conical reaction part, wherein the conical reaction part is used to accommodate a protein sample, and the volume of the conical reaction part is less than 100 μL.

[0008] In this way, it is ensured that the reaction solution fully contacts the protein sample when flowing through the conical reaction part, thereby improving the reaction effect.

[0009] In certain embodiments, the solid phase extraction column further comprises a conical liquid storage portion, a smaller end of the conical liquid storage portion is connected to a larger end of the conical reaction portion, and the volume and taper of the conical liquid storage portion are greater than the volume and taper of the conical reaction portion.

[0010] In this way, it can be ensured that the solid phase extraction column has enough storage reaction solution to act on the protein sample with a sufficient flux of reaction solution. In addition, such a setting can ensure that the solid phase extraction column has a smaller height while having a larger volume of conical liquid storage part and a smaller volume of conical reaction part, thereby ensuring the cleaning effect of the protein sample.

[0011] In some embodiments, the solid phase extraction column includes a first flange portion, which is connected to an end of the conical liquid storage portion away from the conical reaction portion, and the first flange portion is used to bear on a fixing hole of a tray to fix the solid phase extraction column on the tray.

[0012] In this way, the first flange portion is stably attached to the tray, thereby ensuring that the solid phase extraction column works stably.

[0013] In certain embodiments, the solid phase extraction column comprises a filter portion, the filter portion is disposed at an end of the solid phase extraction column away from the conical reaction portion, and the filter portion is cooperatively connected to a pressurizing device.

[0014] In this way, air is ensured to enter the solid phase extraction column from the filter tip, thereby accelerating the working efficiency of the solid phase extraction column.

[0015] An embodiment of the utility model provides an extraction device, and the extraction device comprises the solid phase extraction column described in any one of the above embodiments.

[0016] The extraction device, by setting the volume of the solid phase extraction column to be less than 500 μL, can ensure that the protein sample and the reaction solution fully react in the solid phase extraction column for experiments with small-mass protein samples and reaction solutions, thereby reducing waste and lowering costs.

[0017] In certain embodiments, the extraction device comprises:

[0018] A tray, the tray comprising an upper surface and having a plurality of fixing holes formed on the upper surface, each of the fixing holes being used to fix one of the solid phase extraction columns;

[0019] A booster device, the booster device includes a lower surface and is formed with a plurality of air outlet holes on the lower surface, the plurality of air outlet holes correspond one-to-one to the plurality of fixing holes, and the booster device is configured to provide positive air pressure to the plurality of solid phase extraction columns through the plurality of air outlet holes to push the reaction solution in the solid phase extraction columns to be discharged.

[0020] In this way, the extraction device uses a booster device to apply air pressure to the solid phase extraction columns, so that the air pressure in the multiple solid phase extraction columns is greater than the external environmental pressure, so as to squeeze the reaction solutions in the multiple solid phase extraction columns, thereby allowing the reaction solutions in the multiple solid phase extraction columns to flow out quickly. Compared with a centrifuge, the reaction solution flows faster per unit time in the solid phase extraction columns, and the extraction efficiency is higher.

[0021] In some embodiments, the number of the fixing holes is greater than 24.

[0022] In this way, the extraction device can process more than 24 solid phase extraction columns at a time. On the other hand, since the centrifuge has a maximum of 24 channels and can only process 24 solid phase extraction columns at a time, the extraction device can process more solid phase extraction columns at a time compared to using a centrifuge, thereby improving efficiency.

[0023] In certain embodiments, the tray includes a flange that protrudes upward from the edge of the upper surface, and the solid phase extraction column includes a filter portion. When the solid phase extraction column passes through the fixing hole and is placed stably, the top of the filter portion is flush with the top of the flange.

[0024] In this way, it is ensured that the pressurizing device is smoothly connected to the solid phase extraction column, thereby improving the sealing effect and ensuring the pressurizing effect inside the solid phase extraction column.

[0025] In certain embodiments, the pressurizing device is formed with a communication cavity, the communication cavity is connected to the plurality of air outlet holes, and the pressurizing device is configured to pressurize the communication cavity so that air is discharged from the plurality of air outlet holes.

[0026] In this way, the multiple gas outlets are guaranteed to work normally, thereby ensuring that the reaction solutions of multiple solid phase extraction columns are discharged simultaneously, thereby improving the efficiency.

[0027] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0029] Figure 1 It is a front view of a solid phase extraction column according to an embodiment of the utility model;

[0030] Figure 2 It is a schematic structural diagram of a solid phase extraction column according to an embodiment of the utility model;

[0031] Figure 3It is a structural schematic diagram of a tray according to an embodiment of the utility model;

[0032] Figure 4 It is a schematic diagram of the structure after the tray and the solid phase extraction column are assembled according to the embodiment of the utility model;

[0033] Figure 5 It is a front view of the tray and the solid phase extraction column after being assembled according to the embodiment of the utility model;

[0034] Figure 6 It is a front view of the extraction device according to the embodiment of the utility model;

[0035] Figure 7 It is a schematic structural diagram of an extraction device according to an embodiment of the utility model.

[0036] Description of main component reference numerals:

[0037] Extraction device 100, solid phase extraction column 10, tray 20, pressurizing device 30, conical reaction part 11, conical liquid storage part 12, first flange part 13, filter part 14, upper surface 21, flange 22, lower surface 31, fixing hole 211, and air outlet 311. DETAILED DESCRIPTION

[0038] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0039] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In the description of the present utility model, "multiple" means two or more, unless otherwise clearly and specifically defined.

[0040] In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection. It can be a mechanical connection or an electrical connection. It can be directly connected or indirectly connected through an intermediate medium. It can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0041] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0042] The disclosure herein provides many different embodiments or examples for realizing different structures of the utility model. In order to simplify the disclosure of the utility model, the components and settings of specific examples are described herein. Of course, they are merely examples, and the purpose is not to limit the utility model. In addition, the utility model may repeat reference numbers and / or reference letters in different examples, and such repetition is for the purpose of simplification and clarity, and does not itself indicate the relationship between the various embodiments and / or settings discussed. In addition, the utility model provides various specific examples of processes and materials, but those of ordinary skill in the art may be aware of the application of other processes and / or the use of other materials.

[0043] See also Figure 1 and Figure 2 The embodiment of the utility model provides a solid phase extraction column 10. The volume of the solid phase extraction column 10 is less than 500 μL.

[0044] The solid phase extraction column 10 can ensure that the protein sample and the reaction solution react fully in the solid phase extraction column 10 for the test of small-mass protein samples and reaction solutions by setting the volume of the solid phase extraction column 10 to be less than 500 μL, thereby reducing waste and lowering costs.

[0045] Specifically, in one embodiment, Figure 1 and Figure 2As shown, the solid phase extraction column 10 is a conical sample pretreatment device that can be used to hold protein samples and reaction solutions to ensure that the protein samples and reaction solutions are pressed into appropriate positions on the solid phase extraction column 10, thereby improving the reaction effect of the protein sample and smoothly discharging the reaction solution, thereby obtaining a target polypeptide sample for mass spectrometry analysis.

[0046] For example, in one example, the protein sample can be passed through the reaction solution in the solid phase extraction column 10, and while the protein sample is cleaned, the protein sample can be guaranteed to achieve reduction, alkylation or enzymatic hydrolysis reactions, thereby obtaining the target polypeptide sample.

[0047] That is, the solid phase extraction column 10 should be selected with a suitable volume to reduce the cost while ensuring the processing effect of the protein sample.

[0048] In one embodiment, the volume of the solid phase extraction column 10 is less than 500 μL, which can ensure that the protein sample and the reaction solution react fully to obtain the target polypeptide sample and reduce waste.

[0049] When the volume of the solid phase extraction column 10 is greater than or equal to 500 μL, the volume of the solid phase extraction column 10 is too large, which increases the reaction volume of the protein sample and the reaction solution, thereby increasing the amount of the protein sample and the reaction solution attached to the inner wall of the solid phase extraction column 10, which is easy to cause waste and high processing costs.

[0050] The lower limit of the volume of the solid phase extraction column 10 can be determined based on empirical values ​​and is not specifically limited here. The volume can be less than 500 μL.

[0051] For example, in some examples, the volume of the solid phase extraction column 10 can be 250 μL, 280 μL, 300 μL, 350 μL, 400 μL, 450 μL, 480 μL, or other values ​​less than 500 μL.

[0052] In one embodiment, preferably, the volume of the solid phase extraction column 10 can be 300 μL, which can ensure that the target polypeptide sample formed after the protein sample and the reaction solution are treated meets the use requirements and has low cost.

[0053] In one embodiment, the reaction solution may be a reagent for decomposing a protein sample to convert the protein sample into a target polypeptide sample, which is not particularly limited herein.

[0054] In certain embodiments, the solid phase extraction column 10 includes a conical reaction portion 11, which is used to accommodate a protein sample, and the volume of the conical reaction portion 11 is less than 100 μL.

[0055] In this way, it is ensured that the reaction solution fully contacts the protein sample when flowing through the conical reaction part 11, thereby improving the reaction effect.

[0056] Specifically, in one embodiment, Figure 1 and Figure 2 As shown, the conical reaction part 11 is located at the lower end of the solid phase extraction column 10, and can be used to press and accommodate the protein sample. When the reaction solution flows from top to bottom, the protein sample is fully in contact with the reaction solution, thereby ensuring the reaction effect of the protein sample and the reaction solution.

[0057] That is, the cone-shaped reaction part 11 should be selected with a suitable volume so that the protein sample is fully in contact with the reaction solution to ensure the processing effect of the protein sample and good practicality.

[0058] In one embodiment, the volume of the conical reaction part 11 is less than 100 μL, which can ensure that the protein sample and the reaction solution are fully in contact, so that the protein sample can be fully washed and reacted to obtain the target polypeptide sample.

[0059] When the volume of the conical reaction part 11 is greater than or equal to 100 μL, the volume of the conical reaction part 11 is too large, causing the reaction solution to flow through the protein sample too quickly, thereby reducing the cleaning effect and reaction effect of the protein sample and the reaction solution, that is, the decomposition effect of the protein sample is poor, and the target polypeptide sample cannot meet the requirements of mass spectrometry analysis.

[0060] The lower limit of the volume of the conical reaction part 11 can be determined based on empirical values ​​and is not specifically limited here. The volume can be less than 100 μL.

[0061] For example, in some examples, the volume of the conical reaction part 11 can be 50 μL, 52 μL, 55 μL, 58 μL, 60 μL, 65 μL, 70 μL or other values ​​less than 100 μL.

[0062] In one embodiment, preferably, the volume of the conical reaction part 11 can be 60 μL, which can ensure that the protein sample and the reaction solution react fully, thereby forming a target polypeptide sample that meets the requirements of mass spectrometry analysis.

[0063] In some embodiments, the solid phase extraction column 10 also includes a conical liquid storage portion 12, the smaller end of the conical liquid storage portion 12 is connected to the larger end of the conical reaction portion 11, and the volume and taper of the conical liquid storage portion 12 are greater than the volume and taper of the conical reaction portion 11.

[0064] In this way, it can be ensured that the solid phase extraction column 10 has enough storage reaction solution to act on the protein sample with a sufficient flux of reaction solution. In addition, such a setting can ensure that the solid phase extraction column 10 has a smaller height while having a larger volume of the conical liquid storage part 12 and a smaller volume of the conical reaction part 11, thereby ensuring the cleaning effect of the protein sample.

[0065] Specifically, in one embodiment, Figure 1 and Figure 2 As shown, the conical liquid storage part 12 is located at the upper end of the solid phase extraction column 10 and on the conical reaction part 11, and can be used to hold the reaction solution to ensure that the reaction solution flows through the protein sample from top to bottom, thereby ensuring normal reaction between the protein sample and the reaction solution.

[0066] In one embodiment, the volume and taper of the conical liquid storage portion 12 are greater than the volume and taper of the conical reaction portion 11 to ensure that the conical liquid storage portion 12 stores a larger volume of reaction solution, and utilizes gravity to flow from top to bottom to the conical reaction portion 11 with a smaller volume, so that the protein sample located in the conical reaction portion 11 is fully in contact with the reaction solution, that is, the reaction solution has a faster liquid flow rate and a longer liquid flow time, so as to ensure that the reaction solution can fully clean and decompose the protein sample, thereby ensuring the reaction effect of the protein sample and the reaction solution, and then obtaining the target polypeptide sample that meets the requirements of mass spectrometry analysis.

[0067] In one embodiment, if Figure 1 and Figure 2 As shown, the smaller end of the conical liquid storage part 12 is connected to the larger end of the conical reaction part 11 to ensure that the solid phase extraction column 10 forms a conical sample pretreatment device, thereby meeting the test of small-mass protein samples and reaction solutions and reducing costs.

[0068] See also Figures 2 to 5 In some embodiments, the solid phase extraction column 10 includes a first flange portion 13, which is connected to one end of the conical liquid storage portion 12 away from the conical reaction portion 11, and the first flange portion 13 is used to bear on the fixing hole 211 of the tray 20 to fix the solid phase extraction column 10 on the tray 20.

[0069] In this way, the first flange portion 13 is stably attached to the tray 20 , thereby ensuring that the solid phase extraction column 10 works stably.

[0070] Specifically, in one embodiment, Figure 2 As shown, the first flange portion 13 is protruded along the circumference of the solid phase extraction column 10 and is located at the upper end of the conical liquid storage portion 12, which can ensure that the solid phase extraction column 10 is vertically placed on the tray 20, and has good practicality.

[0071] In one embodiment, please combine Figures 2 to 4 The first flange portion 13 is arranged in a cylindrical shape. When the solid phase extraction column 10 passes through the fixing hole 211 of the tray 20, the first flange portion 13 is in contact with the surface of the tray 20 at the outer edge of the fixing hole 211, thereby ensuring that the solid phase extraction column 10 is vertically suspended on the tray 20 with good stability.

[0072] It can be understood that the first flange portion 13 is annular and the diameter of the first flange portion 13 is greater than the diameter of the fixing hole 211. When the solid phase extraction column 10 passes through the fixing hole 211, the first flange portion 13 is stuck above the fixing hole 211, so that the conical liquid storage portion 12 and the conical reaction portion 11 are both located above the fixing hole 211, that is, the solid phase extraction column 10 is suspended on the tray 20, and at the same time, the upper end of the conical liquid storage portion 12 can form a gap with the fixing hole 211 to be fixed, thereby avoiding the solid phase extraction column 10 from shaking in the horizontal direction, which is safe and stable.

[0073] See also Figure 2 , Figure 6 and Figure 7 In some embodiments, the solid phase extraction column 10 includes a filter portion 14 , which is disposed at one end of the solid phase extraction column 10 away from the conical reaction portion 11 , and the filter portion 14 is cooperatively connected to the pressurizing device 30 .

[0074] In this way, air is ensured to enter the solid phase extraction column 10 from the filter portion 14 , thereby accelerating the working efficiency of the solid phase extraction column 10 .

[0075] Specifically, in one embodiment, Figure 2 , Figure 6 and Figure 7 As shown, the filter portion 14 is arranged at one end of the solid phase extraction column 10 away from the conical reaction portion 11, that is, the filter portion 14 is arranged above the first flange portion 13 and located at the top of the solid phase extraction column 10, and can be used to cooperate with the booster device 30 to pressurize air into the solid phase extraction column 10, thereby ensuring that the protein sample and the reaction solution are fully contacted and reacted, and finally the reaction solution is filtered out and the target polypeptide sample is obtained.

[0076] In one embodiment, the filter portion 14 is arranged to protrude along the circumference of the solid phase extraction column 10 and is parallel to the first flange portion 13, and a hollow cylindrical contact surface can be formed to ensure that the filter portion 14 is correspondingly connected to the air outlet 311 of the boosting device 30, thereby increasing the positive air pressure in the solid phase extraction column 10, thereby accelerating the reaction efficiency of the protein sample.

[0077] It can be understood that the booster device 30 can provide positive air pressure, so that the positive air pressure is input into the solid phase extraction column 10 through the through hole (not shown) formed around the filter portion 14, so that the air squeezes the reaction solution to flow from top to bottom, so as to ensure that the reaction solution contacts and reacts with the protein sample and the reaction solution is discharged downward from the conical reaction portion 11, that is, the booster device 30 can speed up the working efficiency of the solid phase extraction column 10.

[0078] See also Figure 6 and Figure 7 The embodiment of the utility model provides an extraction device 100. The extraction device 100 includes a solid phase extraction column 10 according to any one of the above embodiments.

[0079] The extraction device 100, by setting the volume of the solid phase extraction column 10 to be less than 500 μL, can ensure that the protein sample and the reaction solution fully react in the solid phase extraction column 10 for experiments on small-mass protein samples and reaction solutions, thereby reducing waste and lowering costs.

[0080] It should be noted that the above explanations of the implementation and beneficial effects of the solid phase extraction column 10 are also applicable to the extraction device 100 of the embodiment of the utility model, and will not be repeated here to avoid redundancy.

[0081] In certain embodiments, the extraction device 100 includes a tray 20 and a pressurizing device 30. The tray 20 includes an upper surface 21 and is formed with a plurality of fixing holes 211 on the upper surface 21, each fixing hole 211 being used to fix a solid phase extraction column 10. The pressurizing device 30 includes a lower surface 31 and is formed with a plurality of gas outlet holes 311 on the lower surface 31, and the plurality of gas outlet holes 311 correspond one to one with the plurality of fixing holes 211. The pressurizing device 30 is configured to provide positive gas pressure to the plurality of solid phase extraction columns 10 through the plurality of gas outlet holes 311, so as to push the reaction solution in the solid phase extraction column 10 to be discharged.

[0082] In this way, the extraction device 100 uses the booster device 30 to apply air pressure to the solid phase extraction column 10, so that the air pressure in the multiple solid phase extraction columns 10 is greater than the external environmental pressure, so as to squeeze the reaction solution in the multiple solid phase extraction columns 10, so that the reaction solution in the multiple solid phase extraction columns 10 flows out quickly. Compared with a centrifuge, the reaction solution flows faster per unit time in the solid phase extraction column 10, and the extraction efficiency is higher.

[0083] Specifically, in one embodiment, Figure 3 and Figure 7As shown, the tray 20 includes an upper surface 21 and a plurality of fixing holes 211 are formed on the upper surface 21, wherein the number of the fixing holes 211 is 96, and the fixing holes 211 are regularly arranged in an array of 8 rows and 12 columns on the upper surface 21, which can ensure that 96 solid phase extraction columns 10 can be suspended and placed at the same time at a time, thereby ensuring the stable setting of the solid phase extraction column 10, thereby improving the working efficiency of the solid phase extraction column 10.

[0084] In one embodiment, if Figure 6 and Figure 7 As shown, the pressurizing device 30 includes a lower surface 31 and is formed with a plurality of air outlet holes 311 on the lower surface 31. Correspondingly, the number of the air outlet holes 311 is 96, and the air outlet holes 311 are regularly arranged in an array of 8 rows and 12 columns on the lower surface 31, which can ensure that 96 solid phase extraction columns 10 are covered and sealed at a time, thereby ensuring that the solid phase extraction columns 10 are sealed and pressurized, that is, ensuring that the air pressure is stable and quickly pressed into the solid phase extraction column 10, so as to improve the separation effect of the protein sample and the reaction solution.

[0085] It is understandable that Figure 4 and Figure 7 As shown, the multiple air outlet holes 311 correspond to the multiple fixing holes 211 one by one. When the multiple air outlet holes 311 of the boosting device 30 are in close contact with the multiple solid phase extraction columns 10 located on the tray 20, the boosting device 30 outputs air to the inside of the multiple solid phase extraction columns 10 to squeeze the reaction solution to move from top to bottom, thereby ensuring that the protein sample and the reaction solution are fully contacted and reacted to form a target polypeptide sample, and then the reaction solution is discharged from the lower end of the solid phase extraction column 10 to ensure the separation effect of the protein sample and the reaction solution.

[0086] See also Figures 3 to 5 In some embodiments, the number of fixing holes 211 is greater than 24.

[0087] In this way, the extraction device 100 can process more than 24 solid phase extraction columns 10 at a time. On the other hand, since the centrifuge has only 24 channels at most, it can only process 24 solid phase extraction columns 10 at a time. Therefore, compared with using a centrifuge, the extraction device 100 can process more solid phase extraction columns 10 at a time, thereby improving efficiency.

[0088] Specifically, in one embodiment, Figures 3 to 5 As shown, the number of fixing holes 211 is 96, and the fixing holes 211 are regularly arranged in an array of 8 rows and 12 columns on the upper surface 21, which can ensure that the extraction device 100 can place and process 96 solid phase extraction columns 20 at a time, with high working efficiency, good safety and stability.

[0089] It can be understood that the number of the fixing holes 211 is greater than 24, so that the extraction device 100 can process more solid phase extraction columns 20 at a time compared to a centrifuge, while ensuring that the solid phase extraction columns 20 complete the protein sample separation process while maintaining a vertical placement, which is practical and efficient.

[0090] See also Figures 3 to 5 In some embodiments, the tray 20 includes a flange 22 that protrudes upward from the edge of the upper surface 21, and the solid phase extraction column 10 includes a filter portion 14. When the solid phase extraction column 10 passes through the fixing hole 211 and is placed stably, the top of the filter portion 14 is flush with the top of the flange 22.

[0091] In this way, it is ensured that the pressurizing device 30 is smoothly connected to the solid phase extraction column 10 , thereby improving the sealing effect and ensuring the pressurizing effect inside the solid phase extraction column 10 .

[0092] Specifically, in one embodiment, Figure 4 As shown, at least a portion of the flange 22 is formed on the upper surface 21 and can be symmetrically arranged around the periphery of the plurality of solid phase extraction columns 10 to improve the placement stability and safety of the plurality of solid phase extraction columns 10 .

[0093] In one embodiment, if Figure 5 As shown, when the solid phase extraction column 10 passes through the fixing hole 211 and is placed stably, the top of the filter portion 14 is flush with the top of the flange 22, that is, the tops of the plurality of solid phase extraction columns 10 and the tops of the flange 22 form a flat surface, which can be closely fitted with the lower surface 31 of the boosting device 30 (such as Figure 7 As shown), the boosting device 30 is ensured to stably boost the pressure into the multiple solid phase extraction columns 10, thereby avoiding leakage caused by the loose fit between the gas outlet 31 and the solid phase extraction column 10.

[0094] That is, the flat surface formed by the multiple solid phase extraction columns 10 and the top of the flange 22 fits tightly with the lower surface 31 of the booster 30, which can ensure that the booster 30 is connected to the solid phase extraction column 10 smoothly, thereby ensuring the normal operation of the booster 30, and further improving the reaction efficiency and separation efficiency of the protein sample and the reaction solution.

[0095] See also Figure 7 In some embodiments, the boosting device 30 is formed with a connecting cavity (not shown), which is connected to the multiple air outlet holes 311. The boosting device 30 is configured to pressurize the connecting cavity (not shown) so that air is discharged from the multiple air outlet holes 311.

[0096] In this way, the multiple gas outlets 311 can be guaranteed to work normally, thereby ensuring that the reaction solutions of the multiple solid phase extraction columns 10 are discharged simultaneously, thereby improving the efficiency.

[0097] Specifically, in one embodiment, Figure 6 and Figure 7 As shown, a connecting cavity (not shown) may be formed in the pressure boosting device 30, and the pressure boosting device 30 may be used to concentrate compressed air to provide positive air pressure to the plurality of solid phase extraction columns 10, thereby accelerating the reaction rate of the protein sample and the reaction solution.

[0098] It can be understood that a plurality of air outlet holes 311 are formed on the lower surface 31 of the boosting device 30, and the plurality of air outlet holes 311 are all connected to the connecting cavity, so that compressed air can be input into a plurality of solid phase extraction columns 10 through the plurality of air outlet holes 311, thereby ensuring the normal operation of the protein sample and the reaction solution, and further allowing the reaction solution to be quickly discharged to be separated from the protein sample.

[0099] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiments or examples are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0100] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A solid phase extraction column, characterized in that: The volume of the solid phase extraction column is less than 500 μL, and the solid phase extraction column includes a conical reaction part, and the conical reaction part is used to accommodate a protein sample, and the volume of the conical reaction part is less than 100 μL. The solid phase extraction column also includes a conical liquid storage part, and the smaller end of the conical liquid storage part is connected to the larger end of the conical reaction part, and the volume and taper of the conical liquid storage part are greater than the volume and taper of the conical reaction part.

2. The solid phase extraction column according to claim 1, characterized in that The solid phase extraction column comprises a first flange portion, which is connected to an end of the conical liquid storage portion away from the conical reaction portion, and is used to bear against a fixing hole of a tray to fix the solid phase extraction column on the tray.

3. The solid phase extraction column according to claim 2, characterized in that: The solid phase extraction column comprises a filter portion, which is arranged at one end of the solid phase extraction column away from the conical reaction portion, and the filter portion is cooperatively connected with a pressurizing device.

4. An extraction device, characterized in that: The extraction device comprises the solid phase extraction column according to any one of claims 1 to 3.

5. The extraction device according to claim 4, characterized in that The extraction device comprises: A tray, the tray comprising an upper surface and having a plurality of fixing holes formed on the upper surface, each of the fixing holes being used to fix one of the solid phase extraction columns; A booster device, the booster device includes a lower surface and is formed with a plurality of air outlet holes on the lower surface, the plurality of air outlet holes correspond one-to-one to the plurality of fixing holes, and the booster device is configured to provide positive air pressure to the plurality of solid phase extraction columns through the plurality of air outlet holes to push the reaction solution in the solid phase extraction columns to be discharged.

6. The extraction device according to claim 5, characterized in that The number of the fixing holes is greater than 24.

7. The extraction device according to claim 5, characterized in that The tray includes a flange protruding upward from the edge of the upper surface, and the solid phase extraction column includes a filter portion. When the solid phase extraction column passes through the fixing hole and is stably placed, the top of the filter portion is flush with the top of the flange.

8. The extraction device according to claim 7, characterized in that The pressurizing device is formed with a communication cavity, the communication cavity is connected to the plurality of air outlet holes, and the pressurizing device is configured to pressurize the communication cavity so that air is discharged from the plurality of air outlet holes.

Images