High-flux nucleic acid synthesis reaction device

Abstract

The utility model discloses a high-flux nucleic acid synthesis reaction device, which belongs to the technical field of biomedical research, and comprises a working platform, one end above the working platform is connected with a manipulator, and brackets are connected above the working platform and positioned on two sides of the manipulator; the protective assembly is arranged, a plurality of microporous reaction plates required by DNA synthesis reaction are placed on the placing plate above one of the brackets, and under the action of a sponge pad, friction between the microporous reaction plates and the placing plate can be avoided, the placing plate is prevented from moving under the extrusion action of the microporous reaction plates, and the placing plate moves to drive a vertical rod to move; the vertical rod moves to drive the connecting base plate to move in the connecting cavity, the connecting base plate moves to extrude the first spring, so that the first spring deforms, the microporous reaction plate is conveniently placed, and under the action of the protective frame, the microporous reaction plate can be prevented from being separated from the bracket due to stress, and the placement stability of the microporous reaction plate is ensured.

Description

Technical Field

[0001] The utility model belongs to the technical field of biomedical research, and in particular relates to a high-throughput nucleic acid synthesis reaction device. Background Art

[0002] DNA is a long-chain polymer whose components are four deoxynucleotides, namely: adenine deoxynucleotide, thymine deoxynucleotide, cytosine deoxynucleotide, and guanine deoxynucleotide.

[0003] Chinese patent application No. 202221176329.X discloses an automated high-throughput DNA synthesis reaction device, which relates to the field of biomedical research engineering technology. The automated high-throughput DNA synthesis reaction device includes a liquid adding valve group and a microporous reaction plate; the upper surface of the microporous reaction plate is provided with a plurality of liquid storage sinks, and the bottom of the liquid storage sinks is provided with a plurality of reaction through holes; the liquid adding valve group is arranged above the microporous reaction plate, and the liquid adding valve group includes a piezoelectric valve liquid adding needle and a battery valve liquid adding needle, the piezoelectric valve liquid adding needle is used to add solution to the reaction through hole, and the battery valve liquid adding needle is used to add solution to the liquid storage sink. The automated high-throughput DNA synthesis reaction device of the utility model, on the one hand, utilizes the microporous reaction plate to meet the high-throughput hardware conditions of the multi-hole, and on the other hand, adopts a combination of a piezoelectric valve liquid adding needle spraying a small hole and a battery valve liquid adding needle spraying a large hole to increase the speed and meet the high-throughput requirements of the DNA synthesis reaction.

[0004] In the above patent, the microporous reaction plate is directly placed on the brackets on both sides of the manipulator. During the placement process, the microporous reaction plate is easily separated from the bracket due to external force, which affects the picking operation of the microporous reaction plate and further affects the reaction efficiency. In addition, an observation window is connected to the side wall of the reaction cabinet. Dust and other impurities in the external environment adhere to the observation window, which easily causes the observation window to be contaminated and affects the observation effect of the observation window. Utility Model Content

[0005] In order to solve the problems raised in the above background technology, the utility model provides a high-throughput nucleic acid synthesis reaction device, which has the characteristics of anti-dropping and sealing and shielding.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a high-throughput nucleic acid synthesis reaction device, comprising a working platform, a manipulator is connected to one end above the working platform, brackets are connected to the top of the working platform and on both sides of the manipulator, a protective component is arranged above the bracket, a reaction cabinet is connected to the other end above the working platform, a mobile reaction mechanism is arranged inside the reaction cabinet, an observation window is connected to the side wall of the reaction cabinet, a sealing shielding component is arranged on one side of the reaction cabinet and outside the observation window, and a liquid adding valve group is connected to the top of the reaction cabinet.

[0007] Preferably, the protective assembly includes a protective frame, a connecting piece, a vertical rod, a placement plate and a sponge pad. The protective frame is connected to the top of the bracket, a placement plate is provided inside the protective frame, a sponge pad is connected to one side of the placement plate, and a vertical rod is connected to the other side of the placement plate. A connecting piece is provided between the bracket and the vertical rod.

[0008] Preferably, the connecting member includes a first spring, a connecting cavity and a connecting chassis, the end of the vertical rod close to the bracket is connected to the connecting chassis, a connecting cavity is provided inside the bracket and at a position corresponding to the connecting chassis, and a first spring is connected between the connecting cavity and the connecting chassis.

[0009] Preferably, fixing columns are connected inside the connecting cavity and on both sides of the first spring, and through holes are provided on the connecting chassis at positions corresponding to the fixing columns.

[0010] Preferably, the sealing shielding assembly includes a baffle, a bottom block, a U-shaped frame, a plug-in rod, a second spring and an opening. A baffle is provided on one side of the reaction cabinet and at a position corresponding to the observation window. A U-shaped frame is provided on the periphery of the baffle. Openings are provided on the baffle and the U-shaped frame. A plug-in rod is plugged between the two openings. A second spring is connected between the opening and the plug-in rod. One end of the baffle is connected to the bottom block.

[0011] Preferably, a fixing bar is connected to the side wall of the reaction cabinet and is located below the baffle, a guide column is connected between the fixing bar and the U-shaped frame, and a guide hole is provided on the bottom block at a position corresponding to the guide column.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] 1. The utility model is provided with a protective component, and multiple microporous reaction plates required for DNA synthesis reaction are placed on a placement plate above one of the brackets. Under the action of the sponge pad, friction between the microporous reaction plate and the placement plate can be avoided. The placement plate moves due to the squeezing action of the microporous reaction plate. The movement of the placement plate drives the vertical rod to move. The movement of the vertical rod drives the connecting chassis to move inside the connecting cavity. The movement of the connecting chassis squeezes the first spring to deform it, which is convenient for placing the microporous reaction plate. Under the action of the protective frame, the microporous reaction plate can be prevented from being forced to separate from the bracket, thereby ensuring the stability of the placement of the microporous reaction plate.

[0014] 2. The utility model is provided with a sealing shielding component. After the observation window is used, the two plug-in rods are moved toward each other along the opening, the plug-in rods move to squeeze the second spring, and then the bottom block is moved upward. The movement of the bottom block drives the baffle to move and be plugged into the inside of the U-shaped frame. When the bottom block moves to fit the side wall of the U-shaped frame, the two plug-in rods are released, and the second spring loses the external force and returns to its original appearance, driving the plug-in rod to move and be plugged into the inside of the baffle, and the baffle is plugged and fixed to ensure the stability of the baffle placement. Under the action of the baffle, the fixing strip and the U-shaped frame, the observation window can be sealed and shielded to prevent dust and other impurities in the external environment from adhering to the observation window, thereby ensuring the cleanliness of the surface of the observation window, and further ensuring the subsequent use effect of the observation window. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the structure of the utility model;

[0016] Figure 2 It is a cross-sectional view of the bracket of the utility model;

[0017] Figure 3 For this utility model Figure 3 The enlarged view of point A in the middle;

[0018] Figure 4 It is a cross-sectional view of the reaction cabinet of the utility model;

[0019] Figure 5 For this utility model Figure 4 Cross-sectional view in the BB direction;

[0020] Figure 6 For this utility model Figure 5 Enlarged view of point C in the middle.

[0021] In the figure: 1. Manipulator; 2. Liquid adding valve group; 3. Reactor; 4. Working platform; 5. Bracket; 6. Protective assembly; 61. Protective frame; 62. Connector; 621. First spring; 622. Connecting chamber; 623. Connecting chassis; 63. Vertical rod; 64. Placement plate; 65. Sponge pad; 7. Mobile reaction mechanism; 8. Sealing shielding assembly; 81. Baffle; 82. Fixing strip; 83. Bottom block; 84. U-shaped frame; 85. Connecting rod; 86. Second spring; 87. Opening; 9. Observation window. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] Example 1

[0024] See also Figure 1-6 The utility model provides the following technical solutions: a high-throughput nucleic acid synthesis reaction device, comprising a working platform 4, a manipulator 1 is connected to one end above the working platform 4, brackets 5 are connected to the upper side of the working platform 4 and on both sides of the manipulator 1, a protective component 6 is arranged above the bracket 5, a reaction cabinet 3 is connected to the other end above the working platform 4, a mobile reaction mechanism 7 is arranged inside the reaction cabinet 3, an observation window 9 is connected to the side wall of the reaction cabinet 3, a sealing shielding component 8 is arranged on one side of the reaction cabinet 3 and outside the observation window 9, and a liquid adding valve group 2 is connected to the upper side of the reaction cabinet 3.

[0025] Specifically, the protection assembly 6 includes a protection frame 61, a connecting piece 62, a vertical rod 63, a placement plate 64 and a sponge pad 65. The protection frame 61 is connected to the top of the bracket 5, and a placement plate 64 is arranged inside the protection frame 61. A sponge pad 65 is connected to one side of the placement plate 64, and a vertical rod 63 is connected to the other side of the placement plate 64. A connecting piece 62 is arranged between the bracket 5 and the vertical rod 63.

[0026] By adopting the above technical solution, the microporous reaction plate is placed on the placement plate 64. Under the action of the sponge pad 65, friction between the microporous reaction plate and the placement plate 64 can be avoided. The placement plate 64 moves due to the squeezing effect of the microporous reaction plate. The movement of the placement plate 64 drives the vertical rod 63 to move along the bracket 5 under the action of the connecting piece 62, which is convenient for placing the microporous reaction plate. Under the action of the protective frame 61, the microporous reaction plate can be prevented from being forced to separate from the bracket 5, thereby ensuring the stability of the placement of the microporous reaction plate.

[0027] Specifically, the connecting member 62 includes a first spring 621, a connecting cavity 622 and a connecting chassis 623. The end of the vertical rod 63 close to the bracket 5 is connected to the connecting chassis 623. The connecting cavity 622 is provided inside the bracket 5 at a position corresponding to the connecting chassis 623. The first spring 621 is connected between the connecting cavity 622 and the connecting chassis 623.

[0028] By adopting the above technical solution, the vertical rod 63 moves to drive the connecting chassis 623 to move inside the connecting cavity 622. The connecting chassis 623 moves to squeeze the first spring 621, causing it to deform, thereby facilitating the movement of the vertical rod 63 and the placement plate 64.

[0029] Specifically, the interior of the connecting cavity 622 and the two sides of the first spring 621 are connected with fixing columns, and the connecting chassis 623 is provided with through holes at positions corresponding to the fixing columns.

[0030] By adopting the above technical solution, during the movement of the connecting chassis 623, the cooperation of the perforations and the fixing columns can provide a guide for the movement of the connecting chassis 623, thereby improving the stability of the movement of the connecting chassis 623.

[0031] When the present embodiment is used, multiple microporous reaction plates required for DNA synthesis reaction are placed on the placement plate 64 above one of the brackets 5. Under the action of the sponge pad 65, friction between the microporous reaction plate and the placement plate 64 can be avoided. The placement plate 64 is squeezed by the microporous reaction plate and moves. The movement of the placement plate 64 drives the vertical rod 63 to move. The movement of the vertical rod 63 drives the connecting chassis 623 to move inside the connecting cavity 622. The movement of the connecting chassis 623 squeezes the first spring 621 to deform it, which is convenient for placing the microporous reaction plate. Under the action of the protective frame 61, it can be avoided. The microporous reaction plate is forced to separate from the support 5 to ensure the stability of the placement of the microporous reaction plate. In the subsequent synthesis reaction process, the microporous reaction plate is taken by the manipulator 1 and placed in the mobile reaction mechanism 7. Then, an inert gas such as argon or nitrogen is injected into the environment of the microporous reaction plate, and the excess air is discharged. Then, part of the reagent is injected into the microporous reaction plate by the liquid adding valve group 2 to gradually start the synthesis reaction. After the reaction is completed, the microporous reaction plate in the mobile reaction mechanism 7 is taken out by the manipulator 1, and the microporous reaction plate is placed on the placement plate 64 above another support 5.

[0032] Example 2

[0033] The present embodiment is different from the embodiment 1 in that: the sealing shielding assembly 8 comprises a baffle 81, a bottom block 83, a U-shaped frame 84, a plug-in rod 85, a second spring 86 and an opening 87; a baffle 81 is provided on one side of the reaction cabinet 3 and at a position corresponding to the observation window 9; a U-shaped frame 84 is provided on the periphery of the baffle 81; openings 87 are provided on the baffle 81 and the U-shaped frame 84; a plug-in rod 85 is plugged between the two openings 87; a second spring 86 is connected between the opening 87 and the plug-in rod 85; one end of the baffle 81 is connected to the bottom block 83;

[0034] Specifically, a fixing bar 82 is connected to the side wall of the reaction cabinet 3 and is located below the baffle 81. A guide column is connected between the fixing bar 82 and the U-shaped frame 84. A guide hole is provided on the bottom block 83 at a position corresponding to the guide column.

[0035] By adopting the above technical solution, during the downward movement of the bottom block 83, the cooperation of the guide column and the guide hole can provide a guiding effect for the movement of the bottom block 83. When the bottom block 83 moves to fit the side wall of the fixed bar 82, the bottom block 83 is blocked and stops moving, thereby preventing the baffle 81 from continuing to move, thereby preventing the baffle 81 from detaching from the U-shaped frame 84.

[0036] When the present embodiment is used, during the synthesis reaction, it is necessary to observe the reaction effect inside the mobile reaction mechanism 7. First, the two plug-in rods 85 are moved toward each other along the opening 87. The plug-in rods 85 move to squeeze the second spring 86. At the same time, the baffle 81 loses the plug-in fixing force of the plug-in rods 85 and falls down under the action of gravity. The baffle 81 moves downward to drive the bottom block 83 to move downward. After the bottom block 83 moves and fits to the side wall of the fixing strip 82, the bottom block 83 is blocked and stops moving, thereby preventing the baffle 81 from continuing to move. At this time, the observation window 9 is displayed, and the operator can observe the reaction effect inside the mobile reaction mechanism 7 inside the reaction cabinet 3 through the observation window 9. After the observation is completed, the two plug-in rods 85 are moved toward each other along the opening 87. 5. The plug-in rod 85 moves to squeeze the second spring 86, and then moves the bottom block 83 upward. The bottom block 83 moves to drive the baffle 81 to move and plug it into the inside of the U-shaped frame 84. When the bottom block 83 moves to fit the side wall of the U-shaped frame 84, the two plug-in rods 85 are released, and the second spring 86 loses the external force and returns to its original state, driving the plug-in rod 85 to move and plug it into the inside of the baffle 81. The baffle 81 is plugged and fixed to ensure the stability of the placement of the baffle 81. Under the action of the baffle 81, the fixing bar 82 and the U-shaped frame 84, the observation window 9 can be sealed and shielded to prevent dust and other impurities in the external environment from adhering to the observation window 9, thereby ensuring the cleanliness of the surface of the observation window 9 and further ensuring the subsequent use effect of the observation window 9.

[0037] The structure and principle of the mobile reaction mechanism 7 composed of a reaction tank, a crossbeam and a longitudinal beam in the utility model have been disclosed in an automated high-throughput DNA synthesis reaction device disclosed in Chinese patent application No. 202221176329.X, and its working principle is: a crossbeam is arranged at the bottom of the reaction cabinet 3, and a longitudinal beam is arranged on the crossbeam, and the length direction of the crossbeam is perpendicular to the length direction of the longitudinal beam, and a reaction tank is connected above the longitudinal beam. When in use, after the manipulator 1 places the microporous reaction plate into the reaction tank, the crossbeam and the longitudinal beam cooperate to drive the reaction tank to move to the bottom of the liquid adding valve group 2 for synthesis reaction operation.

[0038] The working principle and use process of the utility model are as follows: the utility model places multiple microporous reaction plates required for DNA synthesis reaction on the placement plate 64 above one of the brackets 5. Under the action of the sponge pad 65, friction between the microporous reaction plate and the placement plate 64 can be avoided. The placement plate 64 is squeezed by the microporous reaction plate and moves. The movement of the placement plate 64 drives the vertical rod 63 to move. The movement of the vertical rod 63 drives the connecting chassis 623 to move inside the connecting cavity 622. The movement of the connecting chassis 623 squeezes the first spring 621 to deform it, which is convenient for placing the microporous reaction plate. Under the action of the protective frame 61, the microporous reaction plate can be avoided from being squeezed. The force is separated from the bracket 5 to ensure the stability of the placement of the microporous reaction plate. In the subsequent synthesis reaction process, the robot 1 is used to take the microporous reaction plate and place the microporous reaction plate inside the mobile reaction mechanism 7. Then, an inert gas such as argon or nitrogen is injected into the environment of the microporous reaction plate, and the excess air is discharged. Then, part of the reagent is injected into the microporous reaction plate by the liquid adding valve group 2 to gradually start the synthesis reaction. During the synthesis reaction, it is necessary to observe the reaction effect inside the mobile reaction mechanism 7. First, the two plug-in rods 85 are moved toward each other along the opening 87. The plug-in rod 85 moves to squeeze the second spring 86. At the same time, the baffle 81 loses the plug-in fixing effect of the plug-in rod 85. The bottom block 83 moves downward and moves downward under the action of gravity. The baffle 81 moves downward and drives the bottom block 83 to move downward. After the bottom block 83 moves and fits the side wall of the fixing bar 82, the bottom block 83 is blocked and stops moving, thereby preventing the baffle 81 from continuing to move. At this time, the observation window 9 is displayed, and the operator can observe the reaction effect inside the mobile reaction mechanism 7 inside the reaction cabinet 3 through the observation window 9. After the observation is completed, the two plug-in rods 85 are moved toward each other along the opening 87. The plug-in rod 85 moves to squeeze the second spring 86, and then the bottom block 83 is moved upward. The bottom block 83 moves and drives the baffle 81 to move and be plugged into the inside of the U-shaped frame 84. When the bottom block 83 moves and fits the side wall of the U-shaped frame 84, the two plugs are released. The connecting rod 85 and the second spring 86 restore their original appearance after losing the external force, driving the connecting rod 85 to move and plug into the inside of the baffle 81, and plug and fix the baffle 81 to ensure the stability of the placement of the baffle 81. Under the action of the baffle 81, the fixing bar 82 and the U-shaped frame 84, the observation window 9 can be sealed and shielded to prevent dust and other impurities in the external environment from adhering to the observation window 9, thereby ensuring the cleanliness of the surface of the observation window 9, and further ensuring the subsequent use effect of the observation window 9. After the reaction is completed, the robot 1 is used to take out the microporous reaction plate inside the mobile reaction mechanism 7, and the microporous reaction plate is placed on the placement plate 64 above another bracket 5.

[0039] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A high-throughput nucleic acid synthesis reaction device, comprising a working platform, characterized in that: A manipulator is connected to one end above the work platform, brackets are connected to the work platform and on both sides of the manipulator, a protective component is arranged above the bracket, a reaction cabinet is connected to the other end above the work platform, a mobile reaction mechanism is arranged inside the reaction cabinet, an observation window is connected to the side wall of the reaction cabinet, a sealing shielding component is arranged on one side of the reaction cabinet and outside the observation window, and a liquid adding valve group is connected to the top of the reaction cabinet.

2. A high-throughput nucleic acid synthesis reaction device according to claim 1, characterized in that: The protection component includes a protection frame, a connecting piece, a vertical rod, a placement plate and a sponge pad. The protection frame is connected to the top of the bracket, a placement plate is arranged inside the protection frame, a sponge pad is connected to one side of the placement plate, and a vertical rod is connected to the other side of the placement plate. A connecting piece is arranged between the bracket and the vertical rod.

3. A high-throughput nucleic acid synthesis reaction device according to claim 2, characterized in that: The connecting member includes a first spring, a connecting cavity and a connecting chassis. The connecting chassis is connected to one end of the vertical rod close to the bracket. A connecting cavity is provided inside the bracket at a position corresponding to the connecting chassis. The first spring is connected between the connecting cavity and the connecting chassis.

4. A high-throughput nucleic acid synthesis reaction device according to claim 3, characterized in that: The interior of the connection cavity and both sides of the first spring are connected with fixing columns, and through holes are arranged on the connection chassis at positions corresponding to the fixing columns.

5. A high-throughput nucleic acid synthesis reaction device according to claim 1, characterized in that: The sealing shielding assembly includes a baffle, a bottom block, a U-shaped frame, a plug-in rod, a second spring and an opening. A baffle is arranged on one side of the reaction cabinet and at a position corresponding to the observation window. A U-shaped frame is arranged on the periphery of the baffle. Openings are arranged on the baffle and the U-shaped frame. A plug-in rod is plugged between the two openings. A second spring is connected between the opening and the plug-in rod. One end of the baffle is connected to the bottom block.

6. A high-throughput nucleic acid synthesis reaction device according to claim 5, characterized in that: A fixing strip is connected to the side wall of the reaction cabinet and is located below the baffle, a guide column is connected between the fixing strip and the U-shaped frame, and a guide hole is arranged on the bottom block at a position corresponding to the guide column.

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