The invention discloses a brewing method of pitaya fruit wine, and belongs to the technical field of fruit wine brewing. The method comprises the following steps of treating raw materials and performing cleaning: cleaning the raw materials with deionized sterile water, after cleaning, performing draining, performing cutting into slices, performing pulping, performing canning and macerating, performing enzymolysis, performing clarification, performing fermentation, pouring wine, performing storage, performing aging, adding clarifying liquid for clarification, performing filtration, performing oxidization resistant treatment, performing blending, performing freezing treatment, performing sterilization and plate filtration, performing stability test, performing precise filtration, and performing quality testing and filling: performing quality testing according to the enterprise standard, and performing filling after the pitaya fruit wine is qualified. The pitaya fruit wine brewed by the brewing method disclosed by the invention has the advantages of being good in mouth feel, stable in quality and the like, and the sensory indexes and the physical and chemical indexes of the produced pitaya wine all reach the national standard.