A cup rack with cup separating function, comprising an opening and closing cover, a cup rack body and a plurality of cups stacked with each other, further comprising at least one cup taking opening and a plurality of cup separating pieces, the plurality of cups are arranged in the cup taking opening, and the cup separating pieces are uniformly arranged on the inner wall of the cup taking opening; the cup separating piece comprises a connecting arm and a limiting component, the limiting component comprises a supporting frame, a first limiting piece and a second limiting piece located on the supporting frame, when the cup at the bottom moves downward, the first limiting piece slides and pushes the second limiting piece to form a limit below the cup rim of the previous cup, so that only one cup is taken at a time. The utility model has the advantages of simple structure and low cost, the user can limit the upper cup when taking the cup, only one cup can be taken at the same time, the practical experience of the user is greatly improved, and the waste of the cup is avoided.