Method for preparing diglyceride

A technology of diglyceride and vegetable oil, which is applied in the direction of fermentation, etc., to achieve good industrial application prospects, product cost reduction, and good catalytic effect

Inactive Publication Date: 2010-12-15
JINAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the preparation of diglycerides by catalyzing the hydrolysis of animal oil or vegetable oil with phospholipase A1

Method used

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  • Method for preparing diglyceride
  • Method for preparing diglyceride
  • Method for preparing diglyceride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 100 g of refined soybean oil in a three-necked reactor, add 10 g of water and 0.05 g of phospholipase A1. The middle port of the three-neck reactor is connected with a stirrer, the left port is connected with a condenser tube, and the right port is closed with a ground plug. The reaction temperature was set at 50° C. and the entire stirring speed was 300 r / min to react for 4 hours. After the reaction, the reaction solution was poured into a separatory funnel and allowed to stand for 60 minutes to separate layers. The upper layer is oil layer, and the lower layer is water and enzyme solution. Discharge the lower layer of liquid, transfer the oil layer into a flask, and spin-evaporate to dehydrate and dry at an absolute pressure of 5000 Pa and a temperature of 90° C. for 30 min to obtain crude diglycerides. The acid value of the crude diglyceride was determined to be 36.80 mgKOH / g.

[0029] The crude diglyceride is distilled to remove the fatty acid of the crude dig...

Embodiment 2

[0031] Weigh 100 g of refined soybean oil in a three-necked reactor, add 36 g of water and 0.26 g of phospholipase A1. The middle port of the three-neck reactor is connected with a stirrer, the left port is connected with a condenser tube, and the right port is closed with a ground plug. The reaction temperature was set at 40° C. and the entire stirring speed was 300 r / min to react for 8 hours. After the reaction, the reaction solution was poured into a separatory funnel and allowed to stand for 40 minutes to separate. The upper layer is oil layer, and the lower layer is water and enzyme solution. Discharge the lower layer of liquid, transfer the oil layer into a flask, and spin-evaporate to dehydrate and dry at an absolute pressure of 4000 Pa and a temperature of 80° C. for 40 minutes to obtain crude diglycerides. The acid value of crude diglyceride was determined to be 53.79mgKOH / g.

[0032] The crude diglyceride is distilled to remove the fatty acid of the crude diglyceride...

Embodiment 3

[0034] Weigh 100 g of refined peanut oil in a three-necked reactor, add 40 g of water and 0.18 g of phospholipase A1. The middle port of the three-neck reactor is connected with a stirrer, the left port is connected with a condenser tube, and the right port is closed with a ground plug. The reaction temperature was set at 30° C. and the entire stirring speed was 300 r / min to react for 12 hours. After the reaction, the reaction solution was transferred to a centrifuge for centrifugation and layering. The upper layer is oil layer, and the lower layer is water and enzyme solution. Discharge the lower layer of liquid, transfer the oil layer into a flask, and spin-evaporate to dehydrate and dry at an absolute pressure of 6000 Pa and a temperature of 80° C. for 50 minutes to obtain crude diglycerides. The acid value of crude diglyceride was determined to be 53.36mgKOH / g.

[0035] The crude diglyceride is distilled to remove the fatty acid of the crude diglyceride by molecular distil...

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Abstract

The invention relates to a method for preparing functional fatty, in particular to a preparing method of diacylglycerol. The preparing method comprises the steps that: (1) 50 shares of animal oil or vegetable oil in weight, 5-30 shares of water in weight and 0.03-0.20 shares of phosphatidase A1 in weight are mixed and reacted for 2 to 12 hours under the temperature of 30 to 70 DEG C and stand for1 to 2 hours or centrifugalize to delaminate; (2) the oil layer gained by delamination on the condition of temperature of 80 to 95 DEG C and absolute pressure of 4000 to 6000 Pa is dried for 30 to 60minutes to get crude diacylglycerol. Purified diacylglycerol can be gained through the elimination of free fatty acids under the temperature of 120 to 160 DEG C and absolute pressure of 0.1 to 40 Pa.Phosphatidase A1 used by the invention has fine catalysis effect and the price is only 5 percent to 10 percent of fatty acid enzyme which leads to significant decrease of cost and fine application prospect in industry.

Description

Technical field [0001] The invention relates to the preparation of functional oils and fats, in particular to a preparation method of diglycerides. Background technique [0002] Diacylglycerol (Diacylglyercrol, DAG) is a product obtained by esterifying glycerol with two fatty acids. There are mainly two isomers: 1,3-diglyceride and 1,2-diglyceride. Diglycerides are natural components of oils and fats, and important biological substances. They are precursors for the synthesis of triglycerides, phospholipids and glycolipids, as well as intermediate products of oil metabolism in the body. [0003] In recent years, many medical studies have shown that diglycerides have the functions of regulating blood lipids, preventing arterial thrombosis, inhibiting liver fat accumulation, relieving diabetic nephropathy, losing weight and suppressing appetite. In 2001, ADM, the largest grain and oil processing company in the United States, and KAO (Kao) of Japan established a joint venture company ...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12P7/62
Inventor汪勇赵谋明欧仕益谢林云
OwnerJINAN UNIVERSITY