A kind of plant polysaccharide and its preparation method and application

A technology of plant polysaccharides and polysaccharides, applied in plant raw materials, plant/algae/fungus/moss components, drug combinations, etc., can solve problems such as large side effects and limited clinical application

Inactive Publication Date: 2012-02-01
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the anticoagulant effect of heparin, a relatively high concentration is required in the body to exert its drug effect, and the side effects are large, which limits its clinical application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 prepares polysaccharide PS-1

[0028] Houttuynia cordata, wild chrysanthemum, Capsicum chinensis, Peilan, and Caoguo five flavors of traditional Chinese medicine are compatible in a composition ratio of 15:6:15:10:3, a total of 9.6Kg. Extract by cold immersion in 95% ethanol, filter, put the dregs in a ventilated place at room temperature to dry, then extract with hot water for 3 times, filter, combine the extracts, concentrate, centrifuge, and defreeze the supernatant with trichloroacetic acid The protein is centrifuged, the supernatant is dialyzed with water for 3 days, the dialysate is concentrated to a small volume, and then ethanol is added to reach an alcohol content of 80%, centrifuged, precipitated and freeze-dried to obtain crude polysaccharide. The crude polysaccharide was dissolved by adding an appropriate amount of distilled water, centrifuged, and the supernatant was washed with a DEAE-cellulose column (Cl -1 type, 30×2.5cm) chromatography for...

Embodiment 2

[0030] Example 2 classical pathway complement inhibition test

[0031] Take the serum of healthy adult male volunteers and use VBS 2+ Buffer (barbital buffer, pH=7.4, containing 0.5mM Mg 2+ and 0.15mM Ca 2+ ) was diluted 1:10 as a source of complement for the classical pathway. Rabbit anti-sheep erythrocyte antibody in VBS 2+ The buffer was diluted 1:1000 as hemolysin; sheep red blood cells (SRBC) preserved in Alsever's solution were prepared as 2% SRBC. Precisely weigh about 1mg of PS-1, add VBS 2+ The buffer was dissolved and diluted to 8 concentrations. 100 μL of different concentrations of PS-1 solution and 100 μL of 1:10 complement were pre-incubated at 37°C for 10 minutes, and then 200 μL of VBS were added in sequence 2+ Buffer, 100 μL hemolysin (1:1000) and 100 μL 2% SRBC were placed in a low-temperature high-speed centrifuge after being placed in a water bath at 37°C for 30 minutes, and centrifuged at 5000 rpm and 4°C for 10 minutes. Take 200 μL of the supernata...

Embodiment 3

[0032] Example 3 Alternative Pathway Complement Inhibition Test

[0033] Serum from healthy adult male volunteers was mixed with VBS-Mg-EGTA buffer (barbital buffer, pH=7.4, containing 5mM Mg 2+ and 8mM EGTA) diluted 1:10, as a source of complement for the alternative pathway. Rabbit red blood cells stored in 3.8% sodium citrate solution were prepared into 2% rabbit red blood cells with VBS-Mg-EGTA buffer solution. Accurately weigh about 1 mg of PS-1, add VBS-Mg-EGTA buffer, and dilute to 8 concentrations. After pre-incubating 150 μL of different concentrations of PS-1 solution and 150 μL of 1:10 complement at 37°C for 10 minutes, add 200 μL of 2% rabbit red blood cells, put them in a low-temperature high-speed centrifuge after 30 minutes in a water bath at 37°C, and centrifuge at 5000 rpm and 4°C. 10min. Take 200 μL of the supernatant from each tube in a 96-well plate, and measure the absorbance at 405 nm. At the same time, a PS-1 control group (150 μL PS-1 solution of th...

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PUM

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Abstract

The invention belongs to the field of traditional Chinese medicine, and relates to a plant polysaccharide PS-1, a preparation method thereof and an application in preparation of anti-complement drugs. The present invention is used to prevent and treat severe atypical pneumonia (SARS), which is composed of Houttuynia cordata, wild chrysanthemum, capillary, peilan, and grass fruit, and its ratio is 15:6:15:10:3. PS-1 was obtained by separating and extracting from substances, and it was confirmed by experiments that PS-1 has inhibitory effects on both the classical pathway and the alternative pathway of complement activation, acting on complement C1q, C1r, C1s, C2, C3, C4, C5, and C9 groups , and has no anticoagulant effect. The plant polysaccharide PS-1 described in the invention can be further used as an active ingredient to prepare novel anti-complement drugs.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine and relates to plant polysaccharides, in particular to a plant polysaccharide and its preparation method and application in the preparation of anti-complement drugs. Background technique [0002] Under normal physiological conditions, the function of complement is mainly to attack foreign pathogens, remove immune complexes, and maintain the balance of the body. However, the abnormal activation of the complement system will cause the excessive response of the human immune system, resulting in the damage and inflammation of the body's own normal tissues. Studies have shown that diseases associated with excessive activation of complement include rheumatoid arthritis, senile dementia, systemic lupus erythematosus (SLE), rejection after organ transplantation, multiple organ failure syndrome, acute respiratory distress syndrome (ARDS )Wait. According to reports, in severe atypical pneumonia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/702A61K36/9064A61P37/06A61P31/14A61P11/00
Inventor 陈道峰张婷章蕴毅
Owner FUDAN UNIV
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