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Use of luteolin in preparing anticomplement medicament

A technology of luteolin and anti-complement, which is applied to the new use of luteolin in the preparation of anti-complement medicine, and the field of anti-complement medicine, which can solve the problems such as the unseen anti-complement efficacy of luteolin.

Inactive Publication Date: 2009-01-21
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Taking a comprehensive view of the reports at home and abroad, there is no anti-complement effect of luteolin and complement inhibitory drugs prepared by it.

Method used

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  • Use of luteolin in preparing anticomplement medicament
  • Use of luteolin in preparing anticomplement medicament
  • Use of luteolin in preparing anticomplement medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 prepares luteolin

[0019] Houttuynia cordata, wild chrysanthemum, Capsicum chinensis, Peilan, and Caoguo five flavors of traditional Chinese medicine are compatible according to their composition ratio of 15:6:15:10:3, a total of 19.8Kg. Extract by cold immersion in 95% ethanol, filter, and recover the ethanol under reduced pressure until there is no alcohol smell to obtain 1Kg of ethanol extract. The ethanol extract was suspended in water, and extracted with equal volumes of petroleum ether, ethyl acetate, and n-butanol to obtain petroleum ether fraction (405 g), ethyl acetate fraction (200 g) and n-butanol fraction (100 g). The ethyl acetate fraction (190 g) was subjected to silica gel column chromatography, eluting with petroleum ether-acetone gradient. The fraction eluted with petroleum ether-acetone (8:2) was subjected to repeated silica gel column chromatography (petroleum ether-acetone 7:3), and then purified by Sephadex LH-20 (chloroform-methanol ...

Embodiment 2

[0021] Example 2 classical pathway complement inhibition test

[0022] Take guinea pig serum to VBS 2+ Buffer (barbital buffer, pH=7.4, containing 0.5mM Mg 2+ and 0.15mM Ca 2+ ) was diluted 1:80 as a source of complement for the classical pathway. Rabbit anti-sheep erythrocyte antibody in VBS 2+ The buffer was diluted 1:1000 as hemolysin; sheep red blood cells (SRBC) preserved in Alsever's solution were prepared as 2% SRBC. Precisely weigh 1mg of luteolin, add VBS 2+ The buffer was dissolved (adding 1% DMSO to aid dissolution), and diluted to 8 concentrations. 200 μL of luteolin solutions of different concentrations and 200 μL of 1:80 complement were pre-incubated at 37°C for 10 minutes, then 100 μL of hemolysin (1:1000) and 100 μL of 2% SRBC were added in sequence, and placed in a low-temperature high-speed centrifuge after 30 minutes in a water bath at 37°C , centrifuged at 5000rpm, 4°C for 10min. Take 200 μL of the supernatant from each tube in a 96-well plate, and m...

Embodiment 3

[0023] Example 3 Alternative Pathway Complement Inhibition Test

[0024] Serum was taken from healthy adult male volunteers and mixed with VBS-Mg-EGTA buffer (barbital buffer, pH=7.4, containing 5mM Mg 2+ and 8mM EGTA) diluted 1:10, as a source of complement for the alternative pathway. Rabbit red blood cells stored in 3.8% sodium citrate solution were prepared into 2% rabbit red blood cells with VBS-Mg-EGTA buffer solution. Precisely weigh 1 mg of luteolin, add VBS-Mg-EGTA buffer solution (1% DMSO is added to aid dissolution), and dilute to 8 concentrations. 150 μL of luteolin solutions of different concentrations and 150 μL of 1:10 complement were pre-incubated at 37°C for 10 minutes, then 200 μL of 2% rabbit red blood cells were added, and placed in a low-temperature high-speed centrifuge after 30 minutes in a water bath at 37°C. Centrifuge for 10 min. Take 200 μL of the supernatant from each tube in a 96-well plate, and measure the absorbance at 405 nm. At the same tim...

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Abstract

The invention belongs to the field of traditional Chinese medicine, and relates to a new application of luteolin for preparing an anti-complement medicine. In the invention, the luteolin is separated and extracted from a traditional Chinese medicine formula used for preventing and treating severe acute respiratory syndrome (SARS); the traditional Chinese medicine formula consists of heartleaf houttuynia herb, wild chrysanthemum, herba artemisiae capillaries, fortune eupatorium herb and tsaoko with the weight ratio of 15: 6: 15: 10: 3; and experiments prove the anticomplement activity of the classical pathway and alternative pathway. The compound luteolin contained in the anticomplement medicine can be taken as an active ingredient for preparing a novel anticomplement medicine.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines and relates to an anti-complement drug, in particular to a new application of luteolin in the preparation of an anti-complement drug. Background technique [0002] Under normal physiological conditions, the function of complement is mainly to attack foreign pathogens, remove immune complexes, and maintain the balance of the body. However, the abnormal activation of the complement system will cause the excessive response of the human immune system, resulting in the damage and inflammation of the body's own normal tissues. Studies have shown that diseases associated with excessive activation of complement include rheumatoid arthritis, senile dementia, systemic lupus erythematosus (SLE), rejection after organ transplantation, multiple organ failure syndrome, acute respiratory distress syndrome (ARDS )Wait. According to reports, in severe atypical pneumonia (SARS) and avian influenza cau...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P37/02A61P37/06
Inventor 陈道峰张婷章蕴毅
Owner FUDAN UNIV
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