Gene diagnostic kit for chicken flavor substances and using method thereof

Abstract

The invention relates to a gene diagnostic kit for chicken flavor substances and a using method thereof, which belong to the technical field of molecular biology. The kit comprises a mixture P1 of upstream and downstream primers for PCR amplification of an ADSL gene, a mixture L1 of probes for an LDR reaction of the ADSL gene, a mixture P2 of upstream and downstream primers for the PCR amplification of an A-FABP gene, a mixture L2 of probes for the LDR reaction of the A-FABP gene, Tag enzyme and DNA ligase. The genotypes of the two genes are detected by an LDR method; and the method comprisesthe following steps: performing PCR amplification on a fragment in which SNP locus is positioned; and ligase detection reaction of a PCR product. When a second exon C3484T of the ADSL gene mutates, IMP is impacted obviously, and the IMP content of a TT genotype individual is the highest and is higher than that of CC and CT obviously; and when a first exon C51T of the A-FABP gene mutates, IMF is impacted obviously, and the IMP content of the TT genotype individual is the highest and is higher than that of the CC and the CT obviously; thus a broiler individual with TTTT genotype combination hasthe highest content of the chicken flavor substances.

Description

Technical field [0001] The invention relates to a chicken flavour substance gene diagnosis kit and a use method thereof, belonging to the technical field of molecular biology. Background technique [0002] The high-quality broiler industry is a characteristic industry in my country. More and more consumers are beginning to pursue the flavor of chicken. In 2008, the production volume exceeded 4.2 billion, and the market share has accounted for more than 55% of the total broiler production, and it is increasing year by year. In the past, traditional breeding for broiler chicken growth performance and body composition reduced the decline of chicken flavor substances. However, it is difficult to achieve the goal of simultaneous improvement of broiler production, growth performance and chicken flavor substances by traditional breeding methods. At present, with the development of molecular biology technology, the use of molecular genetic markers to assist in the selection of high-quali...

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