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Fused gene(s)

A fusion gene and gene technology, applied in the field of fusion genes, can solve the problems of identification of fusion genes in breast cancer, etc.

Inactive Publication Date: 2010-12-15
AGENCY FOR SCI TECH & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the fact that recurrent translocations that generate fusion genes have not been identified in breast cancer, the present invention provides a novel approach to identify fusion genes based on the analysis of unbalanced copy number changes

Method used

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Embodiment

[0135] Standard molecular biology techniques known in the art and not specifically described are generally as described in Sambrook and Russel, Molecular Cloning: A Laboratory Manual, Cold Springs Harbor Laboratory, New York (2001).

[0136] Array comparative genomic hybridization (a-CGH)

[0137]Oligonucleotide-based array comparative genomic hybridization is an emerging technique designed for high-precision mapping of unbalanced copy number changes (Barrett et al., 2004). CGH based on dividing metaphase chromosomes, cDNA array CGH and BAC clone array CGH have low resolution, which can only detect the boundaries of copy number changes in large genomic distances from 100 kb to several megabases. SNP arrays with high density probes from Affymetrix can be used for copy number analysis, but the probes are mostly selected from intergenic regions and further validation studies are needed to map breakpoints in genes. A recently introduced version of the oligonucleotide CGH array (2...

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Abstract

There is provided at least one isolated fused gene comprising at least one first gene and / or fragment thereof fused to at least one second gene and / or fragment thereof, wherein at least the first and / or the second gene, independently, is selected from the group consisting of: RCC2, CENPF, ARFGEF2, SULF2, MTAP, ATXN7, BCAS3, RPS6KB1, TMEM49, EAP30, a gene having the nucleotide sequence SEQ ID NO:1, and a gene having the nucleic acid SEQ ID NO:2, or a fragment thereof. There is also provided a diagnostic method and / or a kit for detecting the susceptibility, prognosis, and / or to tumour in a subject.

Description

technical field [0001] The present invention relates to fusion genes isolated in tumors, particularly breast tumors. The present invention also provides a test kit for detecting fusion genes for tumor diagnosis and / or prognosis of subjects. Background technique [0002] Chromosomal aberrations (including deletions, duplications, inversions, insertions and translocations) are typical features of many types of cancer. The first task of cancer genome analysis is to identify genes that interfere or play a role in cancer development. Many dysregulated fusion genes have been identified by cloning breakpoints of chromosomal translocations in hematological malignancies and soft tissue sarcomas. Chromosomal translocations cause dysregulation of genes at the junction of breaks leading to neoplastic transformation. Chromosomal translocations have mainly two related molecular consequences: first, the promoter and / or enhancer elements of a gene are placed in the vicinity of an oncogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q2600/178C12Q1/6841C12Q2600/156C07K2319/00C07K14/47C12Q1/6886C12Q2600/118
Inventor 那拉斯瓦姆·帕拉尼萨米卡尔帕纳·瑞姆纳瑞阿南埃迪森·T·刘
Owner AGENCY FOR SCI TECH & RES
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