Fused gene(s)
A fusion gene and gene technology, applied in the field of fusion genes, can solve the problems of identification of fusion genes in breast cancer, etc.
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[0135] Standard molecular biology techniques known in the art and not specifically described are generally as described in Sambrook and Russel, Molecular Cloning: A Laboratory Manual, Cold Springs Harbor Laboratory, New York (2001).
[0136] Array comparative genomic hybridization (a-CGH)
[0137]Oligonucleotide-based array comparative genomic hybridization is an emerging technique designed for high-precision mapping of unbalanced copy number changes (Barrett et al., 2004). CGH based on dividing metaphase chromosomes, cDNA array CGH and BAC clone array CGH have low resolution, which can only detect the boundaries of copy number changes in large genomic distances from 100 kb to several megabases. SNP arrays with high density probes from Affymetrix can be used for copy number analysis, but the probes are mostly selected from intergenic regions and further validation studies are needed to map breakpoints in genes. A recently introduced version of the oligonucleotide CGH array (2...
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