Application of proteasome inhibitor MG132 as esbiothrin insecticide synergist

A technology of proteasome inhibitor and pyrethroid insecticide is applied in a new functional field of proteasome inhibitor MG132, which can solve the problems of increasing the difficulty of insecticides in eradicating pests, aggravating the environment and threats.

Inactive Publication Date: 2011-04-20
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the widespread use and dosage increase of organic pesticides, the occurrence and development of pest resistance have increased, the diffi

Method used

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  • Application of proteasome inhibitor MG132 as esbiothrin insecticide synergist
  • Application of proteasome inhibitor MG132 as esbiothrin insecticide synergist
  • Application of proteasome inhibitor MG132 as esbiothrin insecticide synergist

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 The proteasome inhibitor MG132 increases the sensitivity of deltamethrin-resistant mosquito cells to deltamethrin.

[0021] Normal mosquito C6 / 36 cells were purchased from the China Center for Type Culture Collection. In our laboratory, deltamethrin was used to screen for more than 70 generations, and the deltamethrin-resistant mosquito C6 / 36 cell line was established. The LC50 was 1256.8mg / ml , 8.9 times that of normal mosquito cells.

[0022] The inventor first set up a series of concentration gradients of MG132 (0uM – 4 uM) to act on deltamethrin-resistant mosquito cells, and detected

[0023] Cell viability to determine the appropriate experimental concentration of MG132. like figure 1 As indicated, 1 uM was chosen as the concentration of MG132 in the experiment.

[0024] The experiment was divided into control group and experimental group. Mosquito cells in the experimental group were treated with 1uM proteasome inhibitor MG132, while cells in the con...

Embodiment 2

[0026] Example 2 The proteasome inhibitor MG132 increases the sensitivity of deltamethrin-resistant fourth-instar larvae of deltamethrin-resistant mosquitoes to deltamethrin.

[0027] The sensitive strain of Culex palustris is provided by the Chinese Academy of Military Medical Sciences, has never been exposed to any insecticides, and its LC50 for deltamethrin is 0.008ppm; after dozens of generations of screening with different concentrations of deltamethrin in our laboratory, Different levels of resistant mosquitoes were obtained and cultures were maintained with 0.05 ppm and 0.56 ppm deltamethrin, respectively. The experiment was divided into MG132 control group, deltamethrin control group and MG132+deltamethrin experimental group, with 20 fourth-instar mosquito larvae in each group, and experiments were carried out in three strains of mosquitoes.

[0028] In order to determine the appropriate working concentration of MG132, we exposed mosquito fourth-instar larvae to a seri...

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Abstract

The invention relates to a new function of a proteasome inhibitor MG132, in particular to application of the proteasome inhibitor MG132 as an esbiothrin insecticide synergist. The invention provides a new application of the proteasome inhibitor MG132, which provides a new approach to the development of a novel high-efficiency organic insecticide.

Description

technical field [0001] The invention relates to a new function of the proteasome inhibitor MG132, in particular to the application of the proteasome inhibitor MG132 as a synergist of pyrethroid insecticides. Background technique [0002] The use of organic pesticides is an important means to eliminate agricultural and forestry and sanitary pests, to ensure the increase of agricultural and forestry crops and the health of the people. However, with the widespread use and dosage increase of organic pesticides, the occurrence and development of pest resistance have increased, the difficulty of using pesticides to eliminate pests has been increased, and the damage to the environment and the threat to human health have been exacerbated. Therefore, finding an effective method to increase the sensitivity of pests to organic pesticides is an urgent problem to be solved. [0003] The proteasome is a giant protein complex in living organisms whose main job is to degrade proteins tha...

Claims

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Application Information

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IPC IPC(8): A01N47/12A01N53/08A01P7/04
Inventor 孙艳孙林春焦健华贺骥孙海波于婧马磊沈波朱昌亮
Owner NANJING MEDICAL UNIV
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