Preparation method of medicament-carrying biological membrane

A biofilm and drug-carrying technology, applied in the field of medical materials, can solve the problems of fragile carriers, poor transition to clinical applications, and fast drug release.

Active Publication Date: 2013-03-13
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

HA has many advantages, but its stability is slightly poor, sensitive to strong acid, strong alkali, heat, free radicals and hyaluronidase, and prone to degradation, which limits its application as a drug carrier
Although the derivatives of HA alleviate the shortcomings of HA to a certain extent, they must be completed through complex chemical modifications.
Carriers such as gelatin sponge, gliadin, polylactic acid and other materials have their own shortcomings such as: (1) The drug releases quickly and only maintains the effective concentration for several days
(2) The carrier is fragile
Therefore, none of them have made a good transition to clinical application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Embodiment 1: the preparation method of cefazolin drug-loaded biofilm

[0083] 1 Materials and equipment:

[0084] 1.1 Collagen: produced by Beijing Tianxinfu Medical Equipment Co., Ltd. (purity ≥ 95%)

[0085] 1.2 Glacial acetic acid: Sinopharm Chemical Reagent Co., Ltd.

[0086] 1.3 high speed disperser

[0087] 1.4 Circulating cooling device

[0088] 1.5 freeze dryer

[0089] 1.6 high temperature vacuum oven

[0090] 1.7 Cefazolin

[0091] 2 production method:

[0092] 2.1 Slurry preparation

[0093] 2.1.1 Raw material thawing:

[0094] The collagen is taken out from the freezer, and the usage amount of the batch is calculated according to the water content, placed in a sterilized plate, and used after thawing at room temperature.

[0095] 2.1.2 Preparation calculation method

[0096] 600ml of acid solution (glacial acetic acid solution, the same below) contains 3-5g of dry collagen

[0097] 2.1.30.05M glacial acetic acid preparation:

[0098] Take 2-6ml ...

Embodiment 2

[0121] Embodiment 2: the preparation method of cephradine drug-loaded biofilm

[0122] 1 Materials and equipment:

[0123] 1.1 Collagen: produced by Beijing Tianxinfu Medical Equipment Co., Ltd. (purity ≥ 99%)

[0124] 1.2 Glacial acetic acid: Sinopharm Chemical Reagent Co., Ltd.

[0125] 1.3 high speed disperser

[0126] 1.4 Circulating cooling device

[0127] 1.5 freeze dryer

[0128] 1.6 high temperature vacuum oven

[0129] 1.7 Cephradine

[0130] 2.1 Slurry preparation

[0131] 2.1.1 Raw material thawing:

[0132] The collagen is taken out from the freezer, and the usage amount of the batch is calculated according to the water content, placed in a sterilized plate, and used after thawing at room temperature.

[0133] 2.1.2 Preparation calculation method

[0134] 600ml acid solution contains 3-5g dry collagen

[0135] 2.1.30.05M glacial acetic acid preparation:

[0136] Take 2-6ml of glacial acetic acid to a 1000ml Erlenmeyer flask, add sterile water to 600ml, s...

Embodiment 3

[0158] Embodiment 3: the preparation method of cefuroxime (sodium) drug-loaded biofilm

[0159] 1 Materials and equipment:

[0160] 1.1 Collagen: produced by Beijing Tianxinfu Medical Equipment Co., Ltd. (purity ≥ 99%)

[0161] 1.2 Glacial acetic acid: Sinopharm Chemical Reagent Co., Ltd.

[0162] 1.3 high speed disperser

[0163] 1.4 Circulating cooling device

[0164] 1.5 freeze dryer

[0165] 1.6 high temperature vacuum oven

[0166] 1.7 Cefuroxime (sodium)

[0167] 2.1 Slurry preparation

[0168] 2.1.1 Raw material thawing:

[0169] The collagen is taken out from the freezer, and the usage amount of the batch is calculated according to the water content, placed in a sterilized plate, and used after thawing at room temperature.

[0170] 2.1.2 Preparation calculation method

[0171] 600ml acid solution contains 3-5g dry collagen

[0172] 2.1.30.05M glacial acetic acid preparation:

[0173] Take 2-6ml of glacial acetic acid to a 1000ml Erlenmeyer flask, add sterile...

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Abstract

The invention discloses a preparation method of a medicament-carrying biological membrane, which comprises the following steps: preparing type I collagen into a porous and degradable collagen spongy carrier by a freeze-drying process by temperature cross-linking; according to the pharmacokinetics of the selected medicaments, adding medicaments with a certain concentration, such as antibiotics, antiepileptic medicaments, nerve growth factors, NMDA (N-methyl-D-aspartic acid) receptor stimulants, NMDA receptor antagonists and the like, into the liquid medicine slowly in the preparation process of slurry, or soaking a biological membrane into the liquid medicine with a certain concentration; adsorbing the medicaments for a period of time; freeze-drying; packaging; and sterilizing in a proper sterilizing mode (gamma rays or epoxy ethane) according to the properties of the medicaments to form a medicament-carrying preparation with a slow-release effect. The invention has wide application prospects in the technical fields of infection prevention, nerve growth promotion, NMDA metabolism, epileptic prevention and the like.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a method for preparing drug-loaded biofilms. Background technique [0002] With the advancement of medical science and technology and the continuous development of medical materials, collagen-based products in the medical field may have achieved significant development this year. Such as the Chinese patent, the patent number is 02121227.9, and the patent name is medical collagen material and its manufacturing process, which discloses a medical collagen material, which is based on bovine tendon tissue, and is treated with impurity removal, cleaning, acid and alkali enzymes, Processes such as temperature cross-linking, freeze-drying and γ-radiation or ethylene oxide sterilization make porous and spongy medical collagen materials, which have the characteristics of stable structure, good biocompatibility and absorbability. At present, it has been clinically used in the repair and an...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61K9/70A61K47/42A61P31/00A61P25/00A61P3/00A61P25/08A61J3/00
Inventor康承桂睢银海
OwnerTIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD