Preparation method of medicament-carrying biological membrane
A biofilm and drug-carrying technology, applied in the field of medical materials, can solve the problems of fast drug release, poor stability, and poor transition to clinical applications.
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Embodiment 1
[0082] Embodiment 1: the preparation method of cefazolin drug-loaded biofilm
[0083] 1 Materials and equipment:
[0084] 1.1 Collagen: produced by Beijing Tianxinfu Medical Equipment Co., Ltd. (purity ≥ 95%)
[0085] 1.2 Glacial acetic acid: Sinopharm Chemical Reagent Co., Ltd.
[0086] 1.3 high speed disperser
[0087] 1.4 Circulating cooling device
[0088] 1.5 freeze dryer
[0089] 1.6 high temperature vacuum oven
[0090] 1.7 Cefazolin
[0091] 2 production method:
[0092] 2.1 Slurry preparation
[0093] 2.1.1 Raw material thawing:
[0094] The collagen is taken out from the freezer, and the usage amount of the batch is calculated according to the water content, placed in a sterilized plate, and used after thawing at room temperature.
[0095] 2.1.2 Preparation calculation method
[0096] 600ml of acid solution (glacial acetic acid solution, the same below) contains 3-5g of dry collagen
[0097] 2.1.30.05M glacial acetic acid preparation:
[0098] Take 2-6ml ...
Embodiment 2
[0122] Embodiment 2: the preparation method of cephradine drug-loaded biofilm
[0123] 1 Materials and equipment:
[0124] 1.1 Collagen: produced by Beijing Tianxinfu Medical Equipment Co., Ltd. (purity ≥ 99%)
[0125] 1.2 Glacial acetic acid: Sinopharm Chemical Reagent Co., Ltd.
[0126] 1.3 high speed disperser
[0127] 1.4 Circulating cooling device
[0128] 1.5 freeze dryer
[0129] 1.6 high temperature vacuum oven
[0130] 1.7 Cephradine
[0131] 2.1 Slurry preparation
[0132] 2.1.1 Raw material thawing:
[0133] The collagen is taken out from the freezer, and the usage amount of the batch is calculated according to the water content, placed in a sterilized plate, and used after thawing at room temperature.
[0134] 2.1.2 Preparation calculation method
[0135] 600ml acid solution contains 3-5g dry collagen
[0136] 2.1.30.05M glacial acetic acid preparation:
[0137] Take 2-6ml of glacial acetic acid to a 1000ml Erlenmeyer flask, add sterile water to 600ml, s...
Embodiment 3
[0159] Embodiment 3: the preparation method of cefuroxime (sodium) drug-loaded biofilm
[0160] 1 Materials and equipment:
[0161] 1.1 Collagen: produced by Beijing Tianxinfu Medical Equipment Co., Ltd. (purity ≥ 99%)
[0162] 1.2 Glacial acetic acid: Sinopharm Chemical Reagent Co., Ltd.
[0163] 1.3 high speed disperser
[0164] 1.4 Circulating cooling device
[0165] 1.5 freeze dryer
[0166] 1.6 high temperature vacuum oven
[0167] 1.7 Cefuroxime (sodium)
[0168] 2.1 Slurry preparation
[0169] 2.1.1 Raw material thawing:
[0170] The collagen is taken out from the freezer, and the usage amount of the batch is calculated according to the water content, placed in a sterilized plate, and used after thawing at room temperature.
[0171] 2.1.2 Preparation calculation method
[0172] 600ml acid solution contains 3-5g dry collagen
[0173] 2.1.30.05M glacial acetic acid preparation:
[0174] Take 2-6ml of glacial acetic acid to a 1000ml Erlenmeyer flask, add sterile...
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