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PH-sensitive reduction responsive nanogel and preparation method thereof

A nanogel and nanohydrogel technology, which is applied in the directions of inactive medical preparations, pharmaceutical formulations, inactive macromolecular compounds, etc., can solve the problems of premature disintegration of nanoparticles and low particle stability. , to achieve the effect of good stability

Inactive Publication Date: 2013-03-20
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The stability of the particles is not high, which can easily lead to premature disintegration of nanoparticles in the body, and the early release of drugs or active agents

Method used

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  • PH-sensitive reduction responsive nanogel and preparation method thereof
  • PH-sensitive reduction responsive nanogel and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0050] (1) Take 1% (W / V) sodium alginate aqueous solution, add periodate to it according to the molar ratio of the sugar unit of sodium alginate to periodate is 1:0.5, and keep away from light at 25°C After stirring for 8 h, ethylene glycol was added to terminate the reaction. Then, ethanol was added to precipitate a precipitate, and a white product was obtained by suction filtration under reduced pressure. The product was re-dissolved in deionized water, re-precipitated with ethanol, and dried to obtain dialdehyde sodium alginate with an oxidation degree of 44.36%.

[0051] (2) Take the above-mentioned bisaldehyde sodium alginate aqueous solution with a weight percentage of 0.3%, and add the ethanol of 4-mercaptoaniline according to the molar ratio of the aldehyde group of bisaldehyde sodium alginate to the amine group of 4-mercaptoaniline at 1:1. solution, after mixing and stirring for 6 hours, add NaBH 4 , adjusted the pH value to 7.0 with 0.1M HCl, controlled the tempera...

Embodiment 2

[0054] (1) Take 2% (W / V) sodium alginate aqueous solution, add periodate to it according to the molar ratio of the sugar unit of sodium alginate to periodate is 1:0.5, and keep away from light at 25°C After stirring for 8 h, ethylene glycol was added to terminate the reaction. Then, ethanol was added to precipitate a precipitate, and a white product was obtained by suction filtration under reduced pressure. The product was re-dissolved in deionized water, re-precipitated with ethanol, and dried to obtain dialdehyde sodium alginate with an oxidation degree of 42.57%.

[0055] (2) Take the above-mentioned bisaldehyde sodium alginate aqueous solution with a weight percentage of 0.2%, according to the molar ratio of the aldehyde group of bisaldehyde sodium alginate to the amine group of 4-mercaptoaniline is 1:0.75, add 4-mercaptoaniline ethanol solution, after mixing and stirring for 6 hours, add NaBH 4 , adjusted the pH value to 7.0 with 0.1M HCl, controlled the temperature bel...

Embodiment 3

[0058] (1) Take 1% (W / V) sodium alginate aqueous solution, add periodate to it according to the molar ratio of sugar unit of sodium alginate to periodate is 1:0.25, and keep away from light at 25°C After stirring for 8 h, ethylene glycol was added to terminate the reaction. Then, ethanol was added to precipitate a precipitate, and a white product was obtained by suction filtration under reduced pressure. The product was re-dissolved in deionized water, re-precipitated with ethanol, and dried to obtain dialdehyde sodium alginate with an oxidation degree of 24.66%.

[0059] (2) Take the above-mentioned dialdehyde sodium alginate aqueous solution with a weight percentage of 0.3%, and add the ethanol of 4-mercaptoaniline according to the molar ratio of the aldehyde group of dialdehyde sodium alginate to the amine group of 4-mercaptoaniline is 1:1.25. solution, after mixing and stirring for 6 hours, add NaBH 4 , adjusted the pH value to 7.0 with 0.1M HCl, controlled the temperatu...

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Abstract

The invention relates to a pH-sensitive reduction responsive nanogel, which comprises a disulfide-bond-crosslinked sodium alginate derivate serving as an active ingredient. A preparation method of the nanogel comprises the following steps of: adding periodate into a sodium alginate aqueous solution according to molar ratio of the glycosyl unit of the sodium alginate aqueous solution to the periodate as 1:(0.01-10), and reacting away from light to obtain dialdehyde sodium alginate; stirring and uniformly mixing a dialdehyde sodium alginate aqueous solution and a 4-aminothiolphenol ethanol solution according to the molar ratio of the aldehyde group of the dialdehyde sodium alginate to the amino group of the 4-aminothiolphenol as 1:(0.01-10) away from light at the temperature of 0-25 DEG C, and then adding sodium borohydride to obtain sulphydryl sodium alginate; and self-assembling the sulphydryl sodium alginate in an aqueous solution and oxidizing the sulphydryl sodium alginate by oxygen in the solution to obtain the nanogel. The nanogel prepared by the invention has stability, pH sensitivity and reduction responsiveness and has a potential application value in the fields of biomedical implants, biological nanotechnology, drug delivery system and the like.

Description

technical field [0001] The invention relates to the field of gel materials, in particular to a method for preparing a pH-sensitive reduction-responsive nano hydrogel. Background technique [0002] Nanogel is a kind of hydrogel particles with a size ranging from 10 to 1000 nanometers and can be dispersed in aqueous solution, usually composed of physically or chemically cross-linked polymer network structures. It not only has high water content, good biocompatibility and adjustable chemical and mechanical properties, but also high specific surface area can couple biomacromolecules, cross-linked network can coat therapeutic molecules, and adjustable particle size facilitates the adjustment of its Body distribution. These properties endow nanogels with broad application potential in tissue engineering, biomedical implants, bionanotechnology, and drug delivery systems. [0003] At present, the preparation methods of nanogels can be summarized into physical methods and chemical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08J3/075C08L5/04C08K5/37C08B37/04A61K47/36A61K47/42
Inventor 殷以华常丹雷静郑化孙艳娟张晓花
Owner WUHAN UNIV OF TECH
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