ELISA kit for detecting peroxiredoxin IV antibody in biological sample, method and use thereof

A technology for peroxides and biological specimens, applied in the field of medical testing, can solve the problems of low specificity and sensitivity, low sensitivity, and short storage time of APF antigen sheets, and achieve the effect of low cost and good sensitivity

A technology for peroxides and biological specimens, applied in the field of medical testing, can solve the problems of low specificity and sensitivity, low sensitivity, and short storage time of APF antigen sheets, and achieve the effect of low cost and good sensitivity

CN102346186BActive Publication Date: 2015-05-27ARMY MEDICAL UNIV
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  • ELISA kit for detecting peroxiredoxin IV antibody in biological sample, method and use thereof
  • ELISA kit for detecting peroxiredoxin IV antibody in biological sample, method and use thereof
  • ELISA kit for detecting peroxiredoxin IV antibody in biological sample, method and use thereof

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Embodiment Construction

[0035] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. The antigen-coated human peroxide reductase IV described in this example was obtained by constructing a prokaryotic expression plasmid, transforming the expression bacteria, and fermenting and culturing a large amount of bacterial precipitates containing peroxide reductase IV. Goat anti-human IgM labeled with root peroxidase (HRP) was used as the secondary antibody of the indirect method ELISA; the coating concentration of human peroxide reductase IV and the optimal concentration range of detection serum and the negative and positive critical value were further determined. To confirm, a specific and sensitive detection method for peroxide reductase Ⅳ antibody in rheumatoid arthritis was developed, which can capture 7.8 ng / mL peroxide reductase Ⅳ anti...

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Abstract

Provided is an ELISA reagents kit for detecting peroxiredoxin IV antibody in biological samples, comprising an antibody capture agent, an enzyme-labeled secondary antibody and a substrate. Also provided are applications for the kit in detecting volumes of peroxiredoxin IV antibody and rheumatoid arthritis.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to an indirect ELISA kit for detecting peroxide reductase IV antibody in a biological sample and its method and application. Background technique [0002] Rheumatoid arthritis (RA) is a systemic autoimmune disease with synovitis as the basic pathological change and characterized by chronic destructive joint disease. Seriously disabled, seriously affecting the quality of life of patients. Rheumatoid arthritis without proper treatment can persist and even lead to joint deformities. Pathogenic antigen-driven autoreactive CD4 as an autoimmune disease + T cell activation is the core pathway in the pathogenesis of rheumatoid arthritis. [0003] For the diagnosis of rheumatoid arthritis, the mainstream still uses the 1987 American College of Rheumatology RA classification criteria, which mainly rely on clinical manifestations and exclude other arthritis to judge RA. In addition, X-ray ch...

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Application Information

Patent Timeline
27 May 2015
Publication
CN102346186B
IPC
G01N33/543
CPC
C07K16/40; G01N33/573; G01N33/577; G01N33/6893; G01N2800/102
Inventors
吴乔; 吴玉章