Kit for quickly examining loop SFTSV (severe fever with thrombocytopenia syndrome bunyavirus) mediated isothermal amplification of severe fever with thrombocytopenia syndrome bunyavirus

Abstract

The invention discloses a kit for quickly examining loop SFTSV (severe fever with thrombocytopenia syndrome bunyavirus) mediated isothermal amplification of severe fever with thrombocytopenia syndrome bunyavirus. The kit consists of components including (1) 2.5 micro-liters of 10X amplification reaction liquid, (2) 2 micro-liters of 25mmol / l dNTP (deoxyribonucleoside triphosphate), (3) 5pmol of F3 outer primer, 5pmol of B3 outer primer, 40pmol of FIP inner primer, 40pmol of BIP inner primer, 20pmol of LF loop primer and 20pmol of LB loop primer, (4) I micro-liter of Bst (bovine somatotropin) DNA (deoxyribonucleic acid) polymerase, (5) 1 micro-liter of reverse transcriptase, (6) 1 micro-liter of fluorescent dye and (7) ddH2O. During usage, RNA (ribonucleic acid) of an examinee is added into the kit, change of colors of a reaction system is observed after amplification, and an examination result is judged accordingly. The examination kit and an examination method have the advantages that examination cost is low, operation is convenient, examination speed is fast, high specificity and high sensitivity identical to that of PCR (polymerase chain reaction) are realized, and scientific evidence for timely diagnosing and treating severe fever with thrombocytopenia syndrome is provided.

Description

technical field [0001] The invention relates to a rapid detection kit for fever with thrombocytopenia syndrome bunyavirus ring SFTSV mediated isothermal amplification, its use method, and its application in the detection of bunyavirus. Background technique [0002] Severe fever with thrombocytopenia syndrome bunyavirus (Severe fever with thrombocytopenia syndrome bunyavirus, SFTSV) has been confirmed as the cause of fever with thrombocytopenia syndrome that has been highly sporadic in Hubei, Henan, Jiangsu, Zhejiang and other regions since 2004. major pathogens. According to relevant research by the National Center for Disease Control and Prevention, the virus belongs to a new virus belonging to the genus Phleboviridae of the family Bunyaviridae. [0003] Similar to other Phleboviruses of the Bunyaviridae family, the genome of SFTSV consists of three single-stranded negative-sense RNA segments, large (L), medium (M), and small (S). The ends of each fragment are complementa...

AI Technical Summary

Problems solved by technology

However, PCR detection technology requires expensive equipment and reagents, and fluorescent quantitative PCR requires expensive probes and complicated operation steps, and has high requirements for personnel's operation level, which is not suitable for rural, mountainous, and county-level applications. Health centers and other front-line adoption

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