Device and method for removing cellulose nitrate film based protein chip luminescent substrate

Abstract

A device for removing a cellulose nitrate film based protein chip luminescent substrate comprises a hollow cylinder and an upper cover, the lower end of the cylinder is closed, the cylinder is provided with an exhaust nozzle connected with a vacuum pump, the external surface of the upper cover is provided with a groove for disposing a protein chip, and vents are arranged in the groove. A method for removing the cellulose nitrate film based protein chip luminescent substrate comprises the following steps: disposing the protein chip subjected to addition of the luminescent substrate and standing in the groove, and turning on the vacuum pump to make the luminescent substrate residual on the right side of the film diafilter and permeate the film under air pressure. According to the removing device, the structure is simple, the operation is convenient, and the luminescent substrate residual on the right side of the film diafilters and permeates the film under the air pressure by forming a negative pressure on the wrong side of the film through the action of the vacuum pump, the signal value of the optical signal detected after removing a liquid film of the luminescent substrate from the surface of the cellulose nitrate film of the chip is about 40% higher than that the signal value of the optical signal detected when the liquid film of the luminescent substrate exists, and the background value is reduced by about 50%.

Description

technical field [0001] The invention relates to protein chip technology, in particular to a device for removing a luminescent substrate and a method for removing the luminescent substrate using the device. Background technique [0002] The basic principle of protein chip technology is to orderly immobilize various proteins on carriers such as titer plates, filter membranes, and glass slides to become a chip for detection, and then use specific labeled proteins or other components to interact with the chip. After rinsing, the components that cannot be combined with the protein on the chip are washed away, and then various signal capture technologies are used to measure the signal intensity of each point on the chip, and the interaction relationship between proteins is analyzed through the signal intensity. To achieve the purpose of determining the function and content of various proteins. In the field of medical diagnosis, protein chips can use the interaction between antige...

AI Technical Summary

Problems solved by technology

Since the liquid film formed by the luminescent substrate has weakening effects such as refraction on the light generated by the reaction, the effective light signal captured by the reader is reduced; in addition, the existence of the liquid film of the luminescent substrate will also increase the background value

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