Method for establishing hypothyroidism Wistar rat eye socket model

A thyroid function and model technology, which is applied in the direction of pharmaceutical formulas, preparations for in vivo tests, medical preparations containing active ingredients, etc., to achieve the effects of simple production, easy repetition, and high success rate

Active Publication Date: 2014-05-07
SHANGHAI PUTUO DISTRICT LIQUN HOSPITAL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, the orbital model of hypothyroidism induced by direct internal irradiation of hyperthyroid animals has not been found in the literature at home and abroad

Method used

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  • Method for establishing hypothyroidism Wistar rat eye socket model
  • Method for establishing hypothyroidism Wistar rat eye socket model
  • Method for establishing hypothyroidism Wistar rat eye socket model

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Experimental program
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Embodiment

[0035] The purpose of the present invention is to simulate hyperthyroidism (hyperthyroidism) in the radioactive iodine-131 ( 131 I) The method of internal irradiation, to establish a simple and effective orbital animal model of hypothyroidism (hypothyroidism), whose pathological manifestations are similar to thyroid-associated ophthalmopathy (TAO), for observation and discussion of the pathogenesis of TAO. The present invention uses human thyroid-stimulating hormone (hTSH) with higher purity to stimulate Wistar rats to form hyperthyroidism, through 131 I intraperitoneal injection of Wistar rats for 2 months, Wistar rats hypothyroidism, observe animal thyroid, orbital tissue, and judge whether the model is successful.

[0036] Materials and Methods

[0037] 1.1 Materials

[0038] 1.1.1 Experimental animals 20 Wistar rats (male, with an average body weight of 220 g) were provided and raised by the Animal Experiment Center of the Institute of Radiological Medicine, Fudan Univer...

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PUM

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Abstract

The invention relates to a method for establishing a hypothyroidism Wistar rat eye socket model. The method comprises the following steps of: (a) performing intraperitoneal injection on a Wistar rat by using human thyroid stimulating hormone to form hyperthyroidism; and (b) performing internal irradiation on the Wistar rat through radioactive 131I. The method has the advantages that an animal model is easy to manufacture, low in cost and easy to repeat; the method is high in success rate, hypothyroidism exists in 85 percent of models, and thyroid tissues have universal characteristics of the hypothyroidism; 100 percent of models have the characteristics of thyroid-associated ophthalmopathy; actual natural courses and states of diseases are simulated, the hyperthyroidism appears, and then the internal irradiation is performed through the 131I to form the hypothyroidism; and in the testing process, the method is low in animal death rate.

Description

technical field [0001] The invention relates to a method for constructing an animal model, in particular to a method for establishing an orbital model of Wistar rats with hypothyroidism. Background technique [0002] clinically 131 If hypothyroidism occurs during the treatment of GD hyperthyroidism, there is a high risk of TAO deterioration, and some patients may induce the occurrence of Graves ophthalmopathy (GO). There is no suitable animal model to observe the pathological changes of such orbital tissues. [0003] long been looking for 131 The cause of hypothyroidism-induced GO after I treatment of GD, and the observation of orbital histological changes in animal models to understand its pathogenesis. Currently, animal models of hyperthyroidism or hypothyroidism and related eye diseases have been produced by various methods. [0004] 1. First of all, the choice of experimental animals is mainly rats, because the thyroid function and hypothalamus-pituitary-thyroid axis ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/24A61K51/00A01K67/027
Inventor 李方都褚俏梅刘兵蔡德生药小萍
Owner SHANGHAI PUTUO DISTRICT LIQUN HOSPITAL
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