35 results about "Thyroid Gland Tissue" patented technology

Methods of identifying malignant thyroid tissue comprising testing a thyroid tissue sample for the expression of at least two genes chosen from CCND2, PCSK2, and PLAB. Kits for use in the disclosed methods are also provided.

The invention provides a traditional Chinese medicine combination for treating hashimoto thyroiditis and relates to the technical field of medicines. The traditional Chinese medicine combination for treating the hashimoto thyroiditis is characterized by comprising, by weight, 5-12g of Chinese Angelica, 6-12g of bupleurum, 10-20g of atractylodes macrocephala koidz, 5-15g of poria cocos, 5-7g of honey-fried licorice root, 5-15g of rhizoma cyperi, 5-10g of prunella vulgaris, 5-15g of white paeony root, 10-20g of codonopsis pilosula, 10-20g of astragalus and 10-15g of ligusticum chuanxiong. Raw materials are mixed in a casserole, and an appropriate amount of water is added to fully soak the raw materials; the raw materials is decocted with strong fire to boil, then the raw materials are decocted with soft fire, a liquid medicine is filtered out, an appropriate amount of water is added to dregs of a decoction, the dregs are decocted for half an hour, a liquid medicine is filtered out, and the liquid medicines generated twice are mixed and fully cooled and taken half an hour before breakfast and two hours after supper respectively. Various ingredients of the traditional Chinese medicine combination for treating the hashimoto thyroiditis are pure traditional Chinese medicines which can soothe the liver, regulate vital energy, strengthen spleen, nourish blood, promote blood circulation to remove blood stasis and soften hardness to dissipate stagnation when combined to be used, the protecting and restoring effects on a thyroid gland tissue are achieved, symptoms of a patient are effectively relieved, the effective rate is 92.5%, and adverse reactions and toxic and side effects are avoided.

Embodiments herein relate in vitro methods of stem cell differentiation into thyroid hormone producing cells and tissues, and methods of use of these cells.

The invention relates to the preparation of 131I-thyroid stimulating hormone (TSH) and an application thereof, belonging to the field of radionuclide therapeutic drugs. The compound 131I-thyroid stimulating hormone is a radioiodine marker for thyroid stimulating hormone with pathoclisis in vivo acting on thyrocyte. A preparation method of the 131I-thyroid stimulating hormone comprises the following steps of: carrying out iodine 131 marking on the thyroid stimulating hormone by utilizing a chloramine-T method, a peroxide oxidation method or an iodogen method, and introducing radionuclide iodine 131 for treatment into tyrosine residues in thyroid stimulating hormone molecules. The marking rate of the prepared 131I-TSH is 85.9 percent, the radiochemical purity after purification achieves more than 90 percent, and the prepared 131I-TSH can be stored stably for more than one week at room temperature; the 131I-TSH is mainly metabolized through the liver and excreted through the kidneys; the percentage of ID / g of the 131I-TSH in the thyroid gland is not obviously reduced in four hours; and the 131I-TSH can be concentrated in thyroid gland issues sealed by an iodine solution. Concerning low / undifferentiated thyroid tumours incapable of iodine uptake, the 131I-TSH can increase the radioiodine uptake of thyroid gland cancer cells, and the radioiodine 131 can be chosen for treatment.

The invention discloses a traditional Chinese medicine for treating chronic lymphatic thyroiditis. The traditional Chinese medicine is prepared from 10-20 g of Chinese thorowax, 15-20 g of angelica, 13-18 g of white paeony root, 15-20 g of nutgrass galingale rhizome, 10-20 g of prunella, 10-20 g of thunbery fritillary bulb, 10-15 g of seed cowherb, 15-20 g of marine algae, 13-16 g of laminaria, and 10-20 g of dried orange peel. The traditional Chinese medicine has a good curative effect, is well-targeted in practice, and has small side effects. Clinical observation of large samples and animal experiment researches at the prior stage prove that the traditional Chinese medicine compound has the multi-target effects of reducing the antibody level of thyroid of patients with chronic lymphatic thyroiditis as well as of blood serum of animal models, reducing lymphocyte soakage of thyroid tissues, and inhibiting apoptosis of thyroid cells, and has definite curative effects. The traditional Chinese medicine achieves better effects of treating chronic lymphatic thyroiditis at subclinical stage and clinical stage, has a good market application potential and a good prospect, and is bound to bring good economic and social benefits.

The present invention provides a purified and isolated nucleic acid encoding a sodium / iodide symporter. The present invention also provides purified sodium / iodide symporter, a vector comprising nucleic acid encoding sodium / iodide symporter, a host cell transformed with the vector, and a method for producing recombinant sodium / iodide symporter. In addition, the present invention provides nucleic acid probes and mixtures thereof specific for sodium / iodide symporter nucleic acid and antibodies immunoreactive with sodium / iodide symporter. The present invention also provides a method for diagnosing and treating thyroid disorders associated with non-functional sodium / iodide symporter. Furthermore, the present invention provides a method for the selective ablation of tissue. The present invention also provides a method for identifying an iodide transport protein in non-thyroid tissue. Finally, the present invention provides a non-human, transgenic model for a thyroid disorder.

Embodiments herein relate in vitro methods of stem cell differentiation into thyroid hormone producing cells and tissues, and methods of use of these cells.

The invention belongs to the field of medical imaging, in particular to a CT image segmentation method. The method comprises the steps that 1, cross section position images before and after thyroid gland enhancement and registering is conducted; 2, a thyroid gland tissue template extracted; 3, thyroid tissues are segmented. The method has the advantages of high speed, accurate and the like, and the technical demands of thyroid gland CT image automatic diagnosis can be met.

The invention discloses a traditional Chinese medicine composition for treating Hashimoto's thyroiditis. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: 33-46 parts of knoxia root, 31-39 parts of sauropus spatulifolius, 25-36 parts of raw rehmannia root, 22-29 parts of antler, 18-27 parts of salvia miltiorrhiza, 12-27 parts of radix phytolaccae, 8-16 parts of radix linderae, 5-12 parts of kaempferia galamga, 3-9 parts of radix bupleuri, 4-12 parts of atractylodes macrocephala and 5-13 parts of pinellia tuber. According to the traditional Chinese medicine composition for treating the Hashimoto's thyroiditis, various ingredients are all pure traditional Chinese medicines, which have the effects of soothing liver and regulating Qi, invigorating spleen and nourishing blood, promoting blood circulation to dispel blood stasis and softening and resolving hard mass through being used together, so that the traditional Chinese medicine composition plays roles in protecting and repairing thyroid tissue, the symptoms of patients are effectively improved or relieved, the aim of treating early-phase and no-thyroid-dysfunction-accompanied Hashimoto's thyroiditis is achieved, and no adverse reaction and toxic or side effects is caused.

The invention discloses a parathyroid gland identification method based on an image fusion technology, and relates to the technical field of medical assistance. According to the parathyroid gland recognition method, a parathyroid gland recognition model obtained through training is used for carrying out feature extraction and fusion on a target fluorescence development image and a target real-scene image of thyroid tissues to be recognized under the same visual field so as to recognize parathyroid glands, and the characteristic that lymph nodes, fat and other tissues on the real-scene image are easy to distinguish is utilized; the deep learning image fusion technology is adopted to fuse the characteristics of the fluorescent development image and the live-action image to identify the parathyroid gland, so that the problem of misrecognition when the parathyroid gland is identified by using a near-infrared self-fluorescent development identification method can be solved, and the parathyroid gland identification and positioning precision is relatively high; and the risk of damaging parathyroid gland tissues in the neck operation of thyroid gland and the like is reduced.

The invention provides a mouse thyroid epithelial cell isolation and culture method. The method includes the steps of: (a) conducting intraperitoneal injection of pentobarbital sodium to kill a mouse, performing alcohol disinfection, than taking out a mouse thyroid tissue connected to a trachea, conducting soaking washing in a precooled sterile double-antibody-containing PBS buffer solution, and stripping the thyroid tissue; (b) carrying out mechanical dissociation of the thyroid tissue, then preheating a mixed enzyme solution and performing shock digestion for 30-40min, using Ficoll to separate thyroid mononuclear cells, and conducting centrifugation to obtain cell precipitate; (c) employing a completely mixed medium to resuspend the cell precipitate, performing inoculation in a treated cell bottle, and carrying out differential adhesion to obtain thyroid epithelial cells; and (d) culturing the purified thyroid epithelial cells in the completely mixed medium. The mouse thyroid epithelial cell isolation and culture method provided by the invention can acquire mouse thyroid epithelial cells with the characteristics of high yield, high purity and long survival time.

The invention discloses a traditional Chinese medicine decoction for treating hashimoto thyroiditis. The traditional Chinese medicine decoction is prepared from the following traditional Chinese medicine raw materials in parts by weight: 33 to 46 parts of radix knoxiae, 31 to 39 parts of sauropus spatulifolius beille, 25 to 36 parts of radix rehmanniae, 22 to 29 parts of cornua cervi pantotrichum, 18 to 27 parts of the root of red-rooted salvia, 12 to 27 parts of pokeberry root, 8 to 16 parts of the root of three-nerved spicebush, 5 to 12 parts of rhizoma kaempferiae, 3 to 9 parts of rhizoma cyperi, 2 to 8 parts of scutellaria baicalensis, 3 to 8 parts of honey-fried licorice root and 2 to 7 parts of herb of pilosella-leaved drymoglossum. According to the traditional Chinese medicine decoction provided by the invention, all components are pure traditional Chinese medicine and are used in combination for soothing liver and regulating Qi, tonifying spleen and nourishing blood, promoting blood circulation to remove blood stasis and resolving hard lump, the traditional Chinese medicine decoction has protection and restoration effects on thyroid tissues, can effectively relieve patient symptoms, and has no adverse reactions and toxic and side effects, the effective rate is 92.5 percent, and the aim of treating early hashimoto thyroiditis without being concomitant with thyroid dysfunctions is realized.

A traditional Chinese medicine for treating chronic lymphocytic thyroiditis, comprising 10-20g of Bupleurum bupleuri, 15-20g of angelica, 13-18g of white peony root, 15-20g of Rhizoma Cyperi, 10-20g of Prunella vulgaris, 10-20g of Zhebei, It is prepared from 10-15g of vaccinia, 15-20g of seaweed, 13-16g of kelp, and 10-20g of tangerine peel. The present invention has good curative effect, strong practical pertinence, and small side effects; the traditional Chinese medicine compound has been proved to reduce the level of serum thyroid autoantibodies in patients with chronic lymphocytic thyroiditis and animal models through previous large-scale clinical observation and animal experimental research, and reduce the level of thyroid tissue lymphocytes. Infiltration, inhibition of thyroid cell apoptosis and other multi-target effects, the curative effect is definite. The invention uses traditional Chinese medicine to treat both subclinical and clinical stages of chronic lymphocytic thyroiditis and plays a good role, has great application market potential and good prospects, and will surely produce good economic and social benefits.

The invention relates to a distant metastasis analysis method for differentiated thyroid carcinoma (DTC), and relates to a distant metastasis analysis system for the DTC. The distant metastasis analysis system comprises a data receiving module for receiving measurement data, a request calculation module for judging whether or not the data is accordant with calculation processing according to a set measurement threshold value, a data calculation module for calculating data, a processing module for performing processing according to a calculation result, and a display module for displaying analysis information. A group of sTg (stimulated thyroglobulin) dynamic change relevant parameters before 131I treatment is calculated in order to eliminate the influences of residual thyroid tissues before successful thyroidectomy through 131I after surgery on the sTg level, and good reference information is provided for the increase of the early diagnosis accuracy of a DTC distant metastasis patient before 131I treatment. Compared with an existing clinically-applied method for analyzing the distant metastasis state of the patient with a single sTg level analysis, the method disclosed by the invention has the advantage that the analysis accuracy and specificity are improved.

A method for diagnosing follicular thyroid carcinoma is described. The method measures protein expression levels of an aminoacyl-tRNA synthetase (ARS) or an aminoacyl-tRNA synthetase complex-interacting multifunctional protein (AIMP) in a sample, such as a thyroid tissue sample, from a subject suspected of having follicular thyroid carcinoma. The protein expression levels of ARS or AIMP are compared to the measured protein expression levels in a control.

The invention discloses parathyroid gland detection equipment. The parathyroid gland detection equipment comprises an equipment box, a detection grab handle and a box cover and further comprises a grab handle controller, an operation controller and a monitoring unit, the top of the equipment box is covered with the box cover, an equipment bin is formed in the top of the interior of the equipment box, and the grab handle controller, the operation controller and the monitoring unit are sequentially assembled in the equipment bin; the monitoring unit is a fluorescence detector, a detection grab handle is further arranged in the equipment bin, and a guide-out light guide fiber and a guide-in light guide fiber are assembled at the end of a detection handle rod of the detection grab handle in an inserted and embedded mode. According to the parathyroid gland detection equipment, the fluorescence wave crest with the wavelength nanometer of 807 + / -1.5% is fed back to be in contact with parathyroid gland interested tissue of a patient, so that a thyroid gland tissue area needing surgical treatment is positioned through the fed-back light source wavelength, the operation is simple, the carrying is convenient, and the parathyroid gland treatment efficiency and the success probability are greatly improved.

The invention discloses the application of MIT and / or DIT as a thyroid cancer marker and a kit. MIT and DIT are synthetic precursor substances of thyroid hormone T4, and the synthetic precursor substances have stable expression and constant existence in thyroid tissue and have high specificity and effectiveness. According to the invention, conditions that expression quantities of the MIT and the DIT are remarkably different in thyroid cancer tissue and thyroid normal tissue and the expression quantities of the MIT and the DIT in the thyroid cancer tissue are 100-200 times lower than the expression quantities of the MIT and the DIT in the normal tissue are found for the first time. Based on this, the MIT and DIT can be used as biomarkers for thyroid cancer detection, corresponding analysismethods can be used for preparing a thyroid cancer detection kit, and a novel technical means is provided for thyroid cancer detection, diagnosis, treatment and prognosis evaluation. According to theinvention, a thyroid cancer sample collected by a puncture needle can be accurately analyzed, and the purpose of ultra-efficient and high-sensitivity detection of the kit is achieved.

The invention investigates therapeutic effects of dioscorea bulbifera extract, namely diosbulbin B, on model rats by establishing models of propylthiouracide (PTU) induced nodular goiter in rats. Thediosbulbin B can achieve effects of greatly improving the thyroid hormone and antibody levels of rats and lowering the nodal degree and has an excellent effect of treating the nodular goiter. The invention further provides an extraction method of the diosbulbin B, and an experimental foundation is laid for developing new drugs for treating the nodular goiter.

The invention relates to a method for establishing a hypothyroidism Wistar rat eye socket model. The method comprises the following steps of: (a) performing intraperitoneal injection on a Wistar rat by using human thyroid stimulating hormone to form hyperthyroidism; and (b) performing internal irradiation on the Wistar rat through radioactive 131I. The method has the advantages that an animal model is easy to manufacture, low in cost and easy to repeat; the method is high in success rate, hypothyroidism exists in 85 percent of models, and thyroid tissues have universal characteristics of the hypothyroidism; 100 percent of models have the characteristics of thyroid-associated ophthalmopathy; actual natural courses and states of diseases are simulated, the hyperthyroidism appears, and then the internal irradiation is performed through the 131I to form the hypothyroidism; and in the testing process, the method is low in animal death rate.

The invention relates to the technical field of medicines, in particular to an application of a pyrazolopyrimidine derivative in treating autoimmune thyroid diseases. The pyrazolopyrimidine derivativehas the good treatment effect on autoimmune thyroid diseases, can significantly reduce the levels of T3, T4, FT3, FT4 and other thyroid hormones in serum, significantly reduces the levels of antibodies TMAb and TGAb in serum, and effectively improves the infiltration condition of lymphocytes in thyroid tissues. The treatment effect of the pyrazolopyrimidine derivative on autoimmune thyroid diseases is equivalent to that of thyroxine or prednisone.

The invention relates to a method for establishing a hypothyroidism Wistar rat eye socket model. The method comprises the following steps of: (a) performing intraperitoneal injection on a Wistar rat by using human thyroid stimulating hormone to form hyperthyroidism; and (b) performing internal irradiation on the Wistar rat through radioactive 131I. The method has the advantages that an animal model is easy to manufacture, low in cost and easy to repeat; the method is high in success rate, hypothyroidism exists in 85 percent of models, and thyroid tissues have universal characteristics of the hypothyroidism; 100 percent of models have the characteristics of thyroid-associated ophthalmopathy; actual natural courses and states of diseases are simulated, the hyperthyroidism appears, and then the internal irradiation is performed through the 131I to form the hypothyroidism; and in the testing process, the method is low in animal death rate.

The invention discloses an application of total glucosides of paeony in preparation of a medicine for treating Graves disease. In an adenovirus (A-sub-Ad) induced Graves disease model expressing thyrotrophin receptor A subunit, white paeony root total glycoside feed with different concentrations is fed at different time, the total duration is 10 weeks, and the result shows that TRAb and TT4 levels of mice subjected to high-dose early treatment (500mg / kg / d) are obviously reduced; a thyroid tissue pathological section shows that the hyperplasia degree of thyroid tissue is obviously relieved; and the mouse heart weight is reduced. Therefore, the animal experiment result disclosed by the invention shows that the total glucosides of paeony can effectively relieve the condition of the Graves disease, and the application of the total glucosides of paeony in preparing the medicine for treating the Graves disease is fully supported.

The invention relates to the technical field of medicines, in particular to the use of pyrazolopyrimidine derivatives in treating autoimmune thyroid diseases. The pyrazolopyrimidine derivatives of the present invention have a good therapeutic effect on autoimmune thyroid diseases, can significantly reduce the levels of thyroid hormones such as T3, T4, FT3, FT4 in serum, and significantly reduce the levels of antibodies TMAb and TGAb in serum, Effectively improve lymphocyte infiltration in thyroid tissue. The therapeutic effect of the pyrazolopyrimidine derivative of the present invention on autoimmune thyroid disease is equivalent to that of thyroxine or prednisone.

The invention discloses a parathyroid gland identification method based on an image fusion technology, and the method comprises the following steps: obtaining depth images of a fluorescent development image and a live-action image, and achieving the three-dimensional reconstruction of a thyroid tissue image based on the depth images, thereby obtaining a three-dimensional thyroid tissue image; correcting and splicing the three-dimensional thyroid tissue image based on the position information corresponding to the fluorescence development image and the real scene image, and generating a thyroid three-dimensional real scene model and a thyroid fluorescence development model; on the basis of a DssdInceptionV3 model, identification of parathyroid gland areas loaded in the thyroid three-dimensional live-action model and the thyroid fluorescence development model is achieved; overlapping fusion operation of the thyroid three-dimensional live-action model carrying parathyroid gland area marks and the thyroid fluorescence development model is realized; recognition of an overlapped parathyroid gland area is realized, and a non-overlapped area is used as a secondary focus area. According to the invention, the parathyroid gland identification accuracy is greatly improved.

The invention provides an imaging method of a thyroid tissue structure. The imaging method comprises the steps that (1) fluorescence marking lipid is used to mark thyroid tissue; and (2) a fluorescence microscope is used to perform scanning imaging on the thyroid tissue so as to display a follicle structure of the thyroid tissue. According to the imaging method of the thyroid tissue structure, bythe adoption of the fluorescence marking lipid, a thyroid cell membrane is made to load the lipid and marked with fluorescence, a dynamic and clear thyroid tissue microscopic structure is provided, and the imaging method can be widely applied to assessment of the thyroid tissue structure of a thyroid disease animal model and has great significance for formulation of a research and control strategyabout pathogenesis of a thyroid disease.

The thyroid puncture device comprises a puncture needle, a negative pressure suction needle tube, a driving device and a shell. The tip of the puncture needle is blocked, and a cutting hole is formed in the puncture needle close to the tip; the driving device comprises a first driving motor, a first speed reducer, a first transmission mechanism, a second driving motor, a second speed reducer and a second transmission mechanism which are arranged in the shell, the first transmission mechanism is connected with the first driving motor through the first speed reducer, the second transmission mechanism is connected with the second driving motor through the second speed reducer, and a control device is arranged in the shell. The thyroid tissue is cut by rotating the puncture needle and utilizing the cutting hole, and is sucked by the negative pressure suction device, so that the thyroid tissue can be sufficiently and quickly obtained, repeated puncture is not needed, the pain of a patient is reduced, electric work is realized, and the operation is simple and convenient.