Determination method for heparin activity

A determination method and heparin technology, applied in the measurement of color/spectral characteristics, etc., can solve the problems of large deviation and unsuitable for the detection of low-activity heparin samples, and achieve the effects of high accuracy, simple operation and improved detection efficiency.
CN103063592BActive Publication Date: 2014-05-07BLOOD TRASFUSION INST CHINESE ACAD OF MEDICAL SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BLOOD TRASFUSION INST CHINESE ACAD OF MEDICAL SCI
Publication Date
2014-05-07

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Abstract

The invention discloses a determination method for heparin activity. The method comprises three steps of treatment of standard substances and samples, establishment of a standard curve and sample measurement, and calculation of heparin activity contents in the samples, wherein reaction systems and time are as follows: (1) 10-40 microliters standard curve establishment solution and samples with 0-0.1 IU / ml heparin activity concentration are mixed with 10-40 microliters antithrombase with 1 IU / ml concentration, and are incubated for 3 minutes at the temperature of 37 DEG C; (2) 10-40 microliters activated bovine blood coagulation factor X with 10 nkat / ml concentration is added for mixing, and is incubated for 1.5 minutes at the temperature of 37 DEG C; (3) 10-40 ml color development substrate S-2765 with 2.5 mmol / L concentration is added for mixing, and is incubated for 3 minutes at the temperature of 37 DEG C; and (4) 10-40 microliters acetic acid with 50% of volume concentration is added for evenly mixing, and then the reaction is stopped. The method has the advantages of lower reagent dosage, high detection efficiency and high precision of measurement results, and is more suitable for detecting the samples with lower heparin activity.
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Description

technical field

[0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a method for measuring heparin activity. Background technique

[0002] Heparin is a polymer composed of two polysaccharides connected alternately. It has a strong anticoagulant effect both in vivo and in vitro. It is widely used clinically as an anticoagulant drug, mainly for the treatment of thrombotic diseases. Treatment and anticoagulant treatment of cardiovascular surgery, hemodialysis, extracorporeal circulation and other processes.

[0003] As a drug, the precise detection of heparin's activity and function is of great significance for the quality control of the production process and the dynamic monitoring of patients in clinical treatment. As a biologically active substance, its detection cannot be done by chemical or physical methods alone, but must be combined with biological detection methods. At present, coagulation method and chromogenic sub...

Claims

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