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In-vitro long-acting labeling method applicable to stichopus japonicus

A labeling method and technology of sea cucumber, which can be applied in application, climate change adaptation, fish farming, etc., can solve problems such as inability to identify, and achieve the effect of wide applicability

Active Publication Date: 2013-06-12
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other labeling methods for sea cucumbers include molecular labeling and fluorescent staining of bone fragments, but their disadvantage is that they cannot be identified on site, and individuals must be collected and detected through indoor experiments or using special testing equipment

Method used

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  • In-vitro long-acting labeling method applicable to stichopus japonicus

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1: Using nylon fishing line and colored plastic labels to mark sea cucumbers

[0018] A nylon fishing line with a diameter of 0.16 mm was selected as the marking line, and plastic labels (weight 1 g) of different colors with a length of 8 mm were used to mark sea cucumbers weighing about 100 g. Put the label on the marking line, use an embroidery needle with a diameter of 0.35mm to penetrate from the mouth of the sea cucumber at a 45° angle to its body, pass through its lime ring and then pass through the position above its head, and insert the marking line Lead through the lime ring, then adjust the knotting position so that the distance between the thread and the sea cucumber body wall does not exceed 0.5cm, sinter the thread with an electric soldering iron, cut off the thread end, and the label installation is completed. The hanging rate of japonicus tags hung by this method is less than 10% within 3 days, and the retained tags can be kept firmly for more ...

Embodiment 2

[0019] Embodiment 2: Using monofilament polypropylene surgical sutures and PIT electronic tags to mark sea cucumbers

[0020] A monofilament polypropylene surgical suture was selected as the marking line, and a 6 mm long, 1 mm diameter PIT electronic tag (weight 2 g) and the marking line were fixed together with a small piece of plastic sleeve to mark about 100 g of sea cucumbers. Use an embroidery needle with a diameter of 0.35mm to insert the non-absorbable surgical suture from the mouth of the sea cucumber at an angle of 45° to its body, pass through the lime ring and pass it out above the head, and lead the marking line through Lime ring, then adjust the knotting position so that the line is no more than 0.5cm away from the body wall of the sea cucumber, tie the knot by hand, cut off the thread end, and the label installation is completed. The hanging rate of japonicus tags hung by this method is less than 13% within 4 days, and the retained tags can be kept firmly for mor...

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Abstract

The invention relates to the technical field of aquaculture, in particular to an in-vitro long-acting labeling method applicable to stichopus japonicas. The method comprises the following steps: penetrating a labeling line from the mouth of the stichopus japonicas; after penetrating a lime ring, penetrating out of the body from the position above the head of the stichopus japonicas; fixing a tag capable of identifying the stichopus japonicas on the penetrated labeling line; and stably closing the labeling line to realize the in-vitro long-acting labeling of the stichopus japonicas. By adoption of the labeling method, the stichopus japonicas individual can maintain the in-vitro tag for more than 3 months, the normal activity of the stichopus japonicas individual is influenced hardly, and various field labeling release tests and other work which needs to accurately identify the stichopus japonicas individual after the stichopus japonicas individual is labeled can be further developed.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to an in vitro long-acting labeling method suitable for japonicus japonicus. Background technique [0002] Sea cucumber is one of the important marine species in my country. It is often necessary to mark, track and identify sea cucumbers in the process of multiplication, breeding and scientific experiments of sea cucumbers. Among the many biomarking methods, the in vitro labeling method is the most intuitive and convenient method to identify and mark organisms, but because the body wall of sea cucumber is soft, there is almost no hard structure in the whole body, and sea cucumber has strong rejection ability, so It is difficult to apply in vitro labeling methods of other marine organisms (such as fish) to sea cucumbers. A successful in vitro labeling method must meet two conditions: the label can persist for a long time; the labeled organism can maintain normal behavioral acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 许强孙璐张立斌杨红生
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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