Method for breeding Ili horses, and kit employed in method

A kit and female parent technology, applied in the field of breeding methods and kits for improving the body size traits of Yili horses, can solve the problems of low horse heritability, long generation interval, difficult to obtain genetic progress, etc., and achieve the improvement of body size traits , the effect of accelerating cultivation

Inactive Publication Date: 2013-07-03
新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves how well it grows horses' muscle by increasing their overall dimensions while also making them smaller for better performance during training or competition.

Problems solved by technology

Technological Problem: Current techniques used during research into improving muscle quality involve feeding high amounts (more than 500 grams) of carbohydrate or protein sources such as fish meal while reducing unwanted substances called phosphorus compounds which may be associated with cardiovascular diseases. These technical problem addressed through these approaches include developing an efficient method for producing healthier mare products without containing excessively expensive ingredients.

Method used

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  • Method for breeding Ili horses, and kit employed in method
  • Method for breeding Ili horses, and kit employed in method
  • Method for breeding Ili horses, and kit employed in method

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Experimental program
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Embodiment Construction

[0012] 1. Acquisition of SNPs related to body size traits of Yili horses

[0013] (1) Extraction of Yili horse genome DNA

[0014] Collect Yili horse blood, use heparin sodium anticoagulant, use Tiangen Blood Genome Kit to extract Yili horse genomic DNA, detect by 0.8% agarose gel electrophoresis, measure its concentration and purity with a nucleic acid protein quantifier, dilute to 50ng / ul, and store spare. (2) Amplification of the target fragment

[0015] Primers were designed according to the genome sequence of the horse included in the NCBI database, including the forward primer F: 5'-TGGAGTCAGTTGGGTTTAATG-3' and the reverse primer R: 5'-GACCGGATAGCATAGAGAGAG-3' to amplify the target fragment. As shown in SEQ ID NO.1, the SNP is located at 170 bp amplified to the target fragment, and the base at this position can be C or T. When the base at this position is C, it can be recognized by the Alu I endonuclease, and the recognition The sequence is AGCT.

[0016] The PCR rea...

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Abstract

The invention discloses a method for breeding Ili horses by detecting the SNP (single nucleotide polymorphism) of the Ili horses, and a kit employed by the method. Through the adoption of the method and the kit, the body size traits, namely body height, body length, chest girth and cannon circumference, of new groups for Ili horse meat can be remarkably enhanced, and the breeding of the new groups for Ili horse meat is promoted.

Description

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Claims

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Application Information

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Owner 新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心
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