Yili Horse Breeding Method and Its Kit

A kit and female parent technology, applied in the field of breeding methods and kits for improving the body size traits of Yili horses, can solve the problems of low horse heritability, long generation interval, and difficulty in obtaining genetic progress, and achieve accelerated breeding and improved Effects of body size traits

Inactive Publication Date: 2017-04-12
新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, as a large animal, the horse has a long generation interval, and the heritability of the main economic traits of the horse is low, so it is difficult to obtain more significant genetic progress by traditional breeding methods.

Method used

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  • Yili Horse Breeding Method and Its Kit
  • Yili Horse Breeding Method and Its Kit
  • Yili Horse Breeding Method and Its Kit

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] 1. Acquisition of SNPs related to body size traits of Yili horses

[0014] (1) Extraction of Yili horse genome DNA

[0015] Collect Yili horse blood, use heparin sodium anticoagulant, use Tiangen Blood Genome Kit to extract Yili horse genomic DNA, detect by 0.8% agarose gel electrophoresis, measure its concentration and purity with a nucleic acid protein quantifier, dilute to 50ng / ul, and store spare. (2) Amplification of the target fragment

[0016] Primers were designed according to the genome sequence of the horse included in the NCBI database, including the forward primer F: 5'-TGGAGTCAGTTGGGTTTAATG-3' and the reverse primer R: 5'-GACCGGATAGCATAGAGAGAG-3' to amplify the target fragment. As shown in SEQ ID NO.1, the SNP is located at 170 bp amplified to the target fragment, and the base at this position can be C or T. When the base at this position is C, it can be recognized by the Alu I endonuclease, and the recognition The sequence is AGCT.

[0017] The PCR rea...

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Abstract

The invention discloses a method for breeding Ili horses by detecting the SNP (single nucleotide polymorphism) of the Ili horses, and a kit employed by the method. Through the adoption of the method and the kit, the body size traits, namely body height, body length, chest girth and cannon circumference, of new groups for Ili horse meat can be remarkably enhanced, and the breeding of the new groups for Ili horse meat is promoted.

Description

technical field [0001] The invention relates to the fields of animal molecular biology and breeding, in particular to a breeding method for improving the body size traits of Yili horses and a kit thereof. Background technique [0002] Horse meat has excellent quality and taste, and is increasingly favored by consumers, with prices rising steadily. In recent years, with the further understanding of the physical and chemical properties and nutritional value of horse meat, their special functions have gradually become clear. Horse meat has more lean meat, less fat, and more unsaturated fatty acids. This unsaturated fatty acid can offset the harmful process of saturated fatty acids to promote cholesterol deposition in blood vessels. The cholesterol content in horse meat is low, and the cholesterol in every 100g of edible meat The content is only 10.4-31.9 mg, so it has a good effect on preventing atherosclerosis. Domestic horse meat consumption is mainly concentrated in Xinjia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 贺三刚李文蓉刘明军耿娟张宁张雪梅刘晨曦郑新宝董红
Owner 新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心
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