A kind of Trichoderma harzianum sch234 strain and preparation method and application

A technology of bacterial strains and bacterial agents, applied in botany equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of difficult to reach, difficult to control, inconvenient movement, etc., and achieve simple application methods, labor saving, The effect of simple production technology

Inactive Publication Date: 2015-08-12
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But for some special diseases, it is difficult to be effective, such as rice sheath blight caused by Rhizoctonia solani Kühn and rape sclerotinia caused by Sclerotinia sclerotiorum, etc.
The difficulty in the prevention and control of such diseases is that, firstly, pathogenic bacteria can produce a dormant structure with strong stress resistance - sclerotia, which can survive in the soil for a long time and are difficult to control; secondly, there are no host populations for these two diseases. Available disease-resistant germplasm resources; the third two kinds of disease bacteria occur in the stems of crops in the middle and late stages of growth. Due to the closure of crops in the late stage of growth, it is inconvenient for people to move in the field, which brings great difficulties to the application of chemical pesticides. And it is difficult for the medicine to reach the diseased part - the stalk

Method used

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  • A kind of Trichoderma harzianum sch234 strain and preparation method and application
  • A kind of Trichoderma harzianum sch234 strain and preparation method and application
  • A kind of Trichoderma harzianum sch234 strain and preparation method and application

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Experimental program
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Embodiment 1

[0031] A preparation method of Trichoderma harzianum Sch234 inoculum, the steps are as follows:

[0032](1) First activate the Trichoderma harzianum strain Sch234 with the preservation number CCTCC NO: M2012055 on the PDA plate at 26 or 27 or 28 or 29 or 30°C for 2 or 3 or 4 days, and then inoculate it on the PDA slope, 27 or 28 or Cultivate at 29°C for 4 or 5 or 6 days, add sterilized deionized water to prepare a spore suspension, add Tween-20 to make the final concentration of Tween-20 in the spore suspension 0.025%, adjust the concentration of the spore suspension up to 1.5×10 6 / ml;

[0033] (2) Mix the dry compost oatmeal (commercially purchased, with a water content of no more than 8%) and chaff (commercially purchased) at a volume ratio of 1:1, add an appropriate amount of water to make the final water content reach 50%, and pack 35cm long, 20cm wide culture bag, 800g / bag, sterilized by autoclaving at 121℃ for 29 or 30 or 31 minutes;

[0034] (3) The prepared spore s...

Embodiment 2

[0050] Embodiment 2: Research on the biological characteristics of bacterial strain Sch234 of the present invention

[0051] 1. Effect of temperature on the growth of Sch234 strain

[0052] Inoculate Sch234 on PDA medium, culture at 28°C for 1 day, use a hole puncher (5mm in diameter) to punch out pieces with hyphae from the edge of the colony, and transfer it to a Petri dish (90mm in diameter) containing 20ml of PDA The center was cultured at 5°C, 10°C, 15°C, 20°C, 25°C, 30°C and 35°C respectively, and the colony diameter was measured every 12 hours by the cross method, with 3 replicates for each treatment. see results figure 2 , indicating that the Sch234 strain grows fastest at 25°C and 30°C, which are suitable growth temperatures, followed by 20°C, slow growth at 10°C, 15°C, and 35°C, and cannot grow at 5°C.

[0053] 2. Effect of pH environment on the growth of Sch234 strain

[0054] The preparation method of pH3-pH5 PDA medium is: after the PDA medium is sterilized, t...

Embodiment 3

[0056] Embodiment 3: Research on the parasitic sclerotia ability of bacterial strain Sch234 of the present invention

[0057] 1. Determination of Sch234 parasitic Sclerotinia hyphae capacity

[0058] Self-activated Trichoderma harzianum Sch234 strain, Sclerotinia Ep-1PNA367 strain (Liu et al, Journal of Virology, 2009, 1981-1991) and Rhizoctonia solani WH-1 strain (Xie et al, BMC Evolutionary Biology, 2008, 8:87) edge, use a 5mm puncher to punch out the mycelial blocks, inoculate the two mycelial blocks on a PDA plate covered with cellophane, with a distance of 5 cm between them, and culture at 20°C. After confrontation culture for 24 hours, Sch234 was in contact with the hyphae of S. sclerotiorum and Rhizoctonia solani; after 36 hours, obvious parasitic phenomenon could be observed, and the mycelium of Sch234 met the hyphae of Sclerotinia and Rhizoctonia solani and grew parallel to it. Form special branches in the direction of the hyphae, and produce a ring structure for par...

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Abstract

The invention discloses trichoderma harzianum Sch234 bactericide as well as a preparation method and an application thereof. The preparation method of the trichoderma harzianum Sch234 bactericide comprises the following steps of: (1) firstly activating a trichoderma harzianum bacterial strain Sch234 on a PDA flat plate, then inoculating the trichoderma harzianum bacterial strain Sch234 on a PDA inclined plane to be cultured, adding deionized water for sterilization, preparing spore suspension, adding tween-20 until final concentration of the tween-20 in the spore suspension is 0.025%, and adjusting the concentration of the spore suspension to be 1.5*10<6> / ml; (2) mixing dry substrate oatmeal with rice husk uniformly, adding water, and carrying out high-pressure steam sterilizing; and (3) inoculating the prepared spore suspension with substrate in ratio of 10ml per bag, cultivating, and drying in the air and grinding, thus the trichoderma harzianum Sch234 bactericide is obtained. The trichoderma harzianum bacterial strain Sch234 has a strong parasitic effect on rice sheath blight disease and hypha and sclerotium of sclerotinia sclerotiorum, control effect of antibiological inoculant to rice sheath blight disease under field cultivation condition is 83%, an application method in soil treatment is simple, labour force is saved, and the trichoderma harzianum bacterial strain Sch234 can be used for producing biopesticide and is safe to people and livestock.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a bacterial strain Trichoderma certzii Sch234 strain used for the biological control of rice sheath blight and rape sclerotinia, and also relates to the preparation of a biocontrol bacteria Trichoderma harzianum Sch234 bacterial agent The method also relates to the application of a Trichoderma harzianum Sch234 strain in the preparation of medicines for treating or preventing rice sheath blight and rape sclerotinia. Background technique [0002] Plants will be infected with various diseases during the growth process, which will reduce the yield and quality to varying degrees. Most of the pathogenic bacteria that cause plant diseases are fungi, which cause more than 85% of plant diseases, such as rice blast, sheath blight, wheat rust, powdery mildew, rape sclerotinia and other important crops. The most serious diseases are caused by Caused ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 姜道宏程家森付艳苹谢甲涛吉悦
Owner HUAZHONG AGRI UNIV
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