Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Alteromonas and method thereby for producing gel-type enteromorpha polysaccharide degrading enzyme by using Alteromonas

A technology for Alternomonas prolifera and prolifera polysaccharide, which is applied in the production of gel-type prolifera polysaccharide degrading enzymes, and in the field of Alteromonas coli, can solve the problems of difficult degradation of prolifera polysaccharides and the like.

Active Publication Date: 2015-06-17
OCEAN UNIV OF CHINA
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] One object of the present invention is to provide a bacterial strain capable of producing gel-type Enteromorpha polysaccharide-degrading enzymes for the current problem that Enteromorpha polysaccharides are difficult to degrade, and to use the enzymes secreted by it to improve the enzymatic preparation system

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alteromonas and method thereby for producing gel-type enteromorpha polysaccharide degrading enzyme by using Alteromonas
  • Alteromonas and method thereby for producing gel-type enteromorpha polysaccharide degrading enzyme by using Alteromonas
  • Alteromonas and method thereby for producing gel-type enteromorpha polysaccharide degrading enzyme by using Alteromonas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: a kind of Alteromonas colwelliana A321 (Alteromonas colwelliana A321), this bacterial strain has the following characteristics: the bacterial strain is a gram-negative rod-shaped bacillus, without capsule, without spores, with terminal flagella, and the size is about 0.3 -0.7μm×1.8-2.4μm; after the strain was cultured on the gel-type Enteromorpha polysaccharide medium at 28°C for 48 hours, the colony diameter was 2-3mm, round milky yellow, smooth edge, raised in the middle, moist and easy to pick; Dropping iodine solution on the cultured plate can produce obvious gel-type enteromorpha polysaccharide degradation transparent circle; strain A321 can hydrolyze starch, is negative for oxidase, cannot oxidize ethanol to acetic acid, and does not produce H 2 S, produces pigment, cannot utilize citrate, malonate, indole test is negative, can utilize α-glucose, cannot utilize cellobiose, sucrose, D-glucose, D-maltose, D-mannose, L- Arabinol, ancient sugar, D-tagato...

Embodiment 2

[0047] Embodiment 2: Its growth characteristic of a kind of Alteromonas colwelliana A321 (Alteromonas colwelliana A321) described in embodiment 1 is: the growth temperature scope of this bacterium is 10-40 ℃, and optimum growth temperature is 28 ℃; The pH range is 3-11, and the optimum growth pH range is 7.

Embodiment 3

[0048] Embodiment 3: a kind of method that Alteromonas colwelliana A321 (Alteromonas colwelliana A321) described in any one of embodiment 1 or 2 produces gel type enteromorpha polysaccharide degrading enzyme, its steps are as follows;

[0049] (1) Inoculate the bacteria stored on the gel-type Enteromorpha polysaccharide medium into the fermentation medium, rotate at 170r / min, and cultivate at 28°C for 24h to obtain the seed solution;

[0050] (2) The seed liquid is inoculated into the fermentation medium with an inoculum size of 8%, cultivated at 170r / min at 28°C for 12-18h; the fermentation liquid is centrifuged at 10000r / min for 5min, the supernatant is taken, and 60% ammonium sulfate is added, Centrifuge overnight at 4°C at 10,000 r / min, and redissolve the precipitate with an equal volume of distilled water to obtain a crude enzyme solution.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a marine bacterium, wherein the bacterium is characterized in that the bacterium is Alteromonas colwelliana A321 separated in seawater in Qingdao sea area. The preserving unit is CCTCC (China Center For Type Culture Collection) for short and the preserving number is M 2012132. The culture provided by the invention has the characteristics of simplicity in nutritional requirement, easiness in cultivation and short generation time. The growth temperature range of the bacterium is 10-40 DEG C, most preferably 28 DEG C. The growth pH range is 4-11, most preferably 7. The invention further discloses a method for producing a gel-type enteromorpha polysaccharide degrading enzyme by the Alteromonas and the gel-type enteromorpha polysaccharide degrading enzyme obtained by the method. The gel-type enteromorpha polysaccharide degrading enzyme produced by the method provided by the invention can efficiently degrade enteromorpha polysaccharide and can be used to preparation of enteromorpha polysaccharides. Compared with chemical methods, the method provided by the invention is simple in preparation process, high in product yield and stable in quality, active groups are not damaged in the preparation process, and activity study and development of polysaccharides are guaranteed.

Description

technical field [0001] The present invention relates to a kind of Alteromonas, especially a kind of Alteromonas colwelliana A321 (Alteromonas colwelliana A321) isolated from the sea area of ​​Qingdao, which has been preserved in Wuhan University, Wuhan, China on April 23, 2012. The Chinese Type Culture Collection Center CCTCC, the preservation number is CCTCC NO: M2012132; the present invention also relates to the method for producing gel-type Enteromorpha polysaccharide degrading enzyme by the strain and its products. Background technique [0002] Entermorpha (Entermorpha) is a large green algae, which is abundant in wild algae resources in my country and is distributed in the southeast coast. In recent years, due to global warming and seawater pollution, the sources of carbon, nitrogen, and phosphorus in seawater have continued to increase, leading to the crazy growth and spread of Enteromorpha. The "green tide" caused by the outbreak of Enteromorpha may also bring a seri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/24C12R1/01
Inventor 王鹏李银平江晓路
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com