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Alteromonas and method thereby for producing gel-type enteromorpha polysaccharide degrading enzyme by using Alteromonas

A technology of Alteromonas and Enteromorpha polysaccharide, which is applied in the field of Alteromonas, can solve the problem of difficult degradation of Enteromorpha polysaccharide

Active Publication Date: 2013-12-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] One object of the present invention is to provide a bacterial strain capable of producing gel-type Enteromorpha polysaccharide-degrading enzymes for the current problem that Enteromorpha polysaccharides are difficult to degrade, and to use the enzymes secreted by it to improve the enzymatic preparation system

Method used

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  • Alteromonas and method thereby for producing gel-type enteromorpha polysaccharide degrading enzyme by using Alteromonas
  • Alteromonas and method thereby for producing gel-type enteromorpha polysaccharide degrading enzyme by using Alteromonas
  • Alteromonas and method thereby for producing gel-type enteromorpha polysaccharide degrading enzyme by using Alteromonas

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Embodiment 1

[0046] Embodiment 1: a kind of alteromonas A321 (Alteromonas cdwelliana A321), this bacterial strain has the following characteristics: bacterial strain is the gram-negative coryneform bacillus, without capsule, without spore, has terminal flagella, and size is about 0.3-0.7 μm×1.8-2.4μm; after the strain was cultured on the gel-type Enteromorpha polysaccharide medium at 28°C for 48 hours, the colony diameter was 2-3mm, round milky yellow, smooth edge, raised in the middle, moist and easy to pick; Adding iodine solution to the final plate can produce obvious gel-type enteromorpha polysaccharide degradation transparent circle; strain A321 can hydrolyze starch, is negative for oxidase, cannot oxidize ethanol to acetic acid, and does not produce H 2 S, produces pigment, cannot utilize citrate, malonate, indole test is negative, can utilize α-glucose, cannot utilize cellobiose, sucrose, D-glucose, D-maltose, D-mannose, L- Arabinol, ancient sugar, D-tagatose, D-trehalose, D-mannito...

Embodiment 2

[0047] Embodiment 2: the growth characteristic of a kind of Alteromonas cdwelliana A321 (Alteromonas cdwelliana A321) described in embodiment 1 is: the growth temperature range of this bacterium is 10-40 ℃, and optimum growth temperature is 28 ℃; growth pH range 3-11, the optimum growth pH range is 7.

Embodiment 3

[0048] Embodiment 3: a kind of method for the production of gel type enteromorpha polysaccharide degrading enzyme by Alteromonas A321 (Alteromonas cdwellianaA321) described in any one of embodiment 1 or 2, its steps are as follows;

[0049] (1) Inoculate the bacteria stored on the gel-type Enteromorpha polysaccharide medium into the fermentation medium, rotate at 170r / min, and cultivate at 28°C for 24h to obtain the seed solution;

[0050] (2) The seed liquid is inoculated into the fermentation medium with an inoculum size of 8%, cultivated at 170r / min at 28°C for 12-18h; the fermentation liquid is centrifuged at 10000r / min for 5min, the supernatant is taken, and 60% ammonium sulfate is added, Centrifuge overnight at 4°C at 10,000 r / min, and redissolve the precipitate with an equal volume of distilled water to obtain a crude enzyme solution.

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Abstract

The invention relates to a marine bacterium, wherein the bacterium is characterized in that the bacterium is Alteromonas colwelliana A321 separated in seawater in Qingdao sea area. The preserving unit is CCTCC (China Center For Type Culture Collection) for short and the preserving number is M 2012132. The culture provided by the invention has the characteristics of simplicity in nutritional requirement, easiness in cultivation and short generation time. The growth temperature range of the bacterium is 10-40 DEG C, most preferably 28 DEG C. The growth pH range is 4-11, most preferably 7. The invention further discloses a method for producing a gel-type enteromorpha polysaccharide degrading enzyme by the Alteromonas and the gel-type enteromorpha polysaccharide degrading enzyme obtained by the method. The gel-type enteromorpha polysaccharide degrading enzyme produced by the method provided by the invention can efficiently degrade enteromorpha polysaccharide and can be used to preparation of enteromorpha polysaccharides. Compared with chemical methods, the method provided by the invention is simple in preparation process, high in product yield and stable in quality, active groups are not damaged in the preparation process, and activity study and development of polysaccharides are guaranteed.

Description

technical field [0001] The present invention relates to an alteromonas, especially an alteromonas A321 (Alteromonas cdwelliana A321) isolated from the sea area of ​​Qingdao, which has been preserved in China's Type Culture Collection Center CCTCC on April 23, 2012, with a preservation number It is CCTCC NO: M 2012132; the present invention also relates to the method for producing gel-type Enteromorpha polysaccharide degrading enzyme by the strain and its products. Background technique [0002] Entermorpha (Entermorpha) is a large green algae, which is abundant in wild algae resources in my country and is distributed in the southeast coast. In recent years, due to global warming and seawater pollution, the sources of carbon, nitrogen, and phosphorus in seawater have continued to increase, leading to the crazy growth and spread of Enteromorpha. The "green tide" caused by the outbreak of Enteromorpha may also bring a series of secondary environmental hazards, so the resource u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/24C12R1/01
Inventor 王鹏李银平江晓路
Owner OCEAN UNIV OF CHINA
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