Efficient antibacterial activity antisense nucleic acid sequence of multidrug resistant Acinetobacter baumannii

A kind of Acinetobacter baumannii, multi-drug resistance technology, used in antibacterial drugs, pharmaceutical formulations, recombinant DNA technology and other directions

Inactive Publication Date: 2014-02-12
夏云 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no highly efficient antisense nucleic acid seq...

Method used

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  • Efficient antibacterial activity antisense nucleic acid sequence of multidrug resistant Acinetobacter baumannii
  • Efficient antibacterial activity antisense nucleic acid sequence of multidrug resistant Acinetobacter baumannii
  • Efficient antibacterial activity antisense nucleic acid sequence of multidrug resistant Acinetobacter baumannii

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Experimental program
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Embodiment Construction

[0018] 1: Synthesis of antisense nucleic acid

[0019] In order to keep the stability of the antisense nucleic acid in the research, it is not easy to be degraded and lose the inhibitory effect, the antisense nucleic acid is synthesized in the form of peptide nucleic acid (PNA) to increase its stability, and the sequence modification of the penetrating peptide KFFKFFKFFK is added to overcome the The bacterial membrane structure acts as a barrier to macromolecules, so as to achieve the purpose of transferring the antisense sequence into the bacterial body in a stable form.

[0020] Peptide nucleic acid (PNA) is an artificially synthesized DNA analog, which replaces the 3', 5' phosphodiester bonds of the natural nucleic acid backbone with peptide bonds (NH-CO), and has high water solubility, high stability, and specificity for base pairing. Sexual characteristics. However, the molecular weight of peptide nucleic acid is much larger than that of commonly used drugs, which makes ...

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PUM

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Abstract

The present invention discloses an antisense oligonucleotide sequence for inhibiting growth of multidrug resistant Acinetobacter baumannii, and an application thereof, and belongs to the field of novel antibacterial preparation research and development. According to the invention, the computer-assisted design and oligonucleotide dot blot hybridization combined technology is adopted to screen the antisense oligonucleotide adopting coding gene gyrA of the multidrug resistant Acinetobacter baumannii topoismerase IIA subunit as target gene so as to inhibit growth of multidrug resistant Acinetobacter baumannii; the antisense oligonucleotide sequence has significant multidrug resistant Acinetobacter baumannii growth inhibition activity at a low concentration, and can present rapid and efficient bactericidal activity (ie., cause death of multidrug resistant Acinetobacter baumannii) when the concentration is increased; and the designed antisense nucleic acid has characteristics of strong inhibition activity and high specificity, is suitable for treatment of multidrug resistant Acinetobacter baumannii infections, and can be potentially developed into the anti-multidrug resistant Acinetobacter baumannii infection drug.

Description

technical field [0001] The invention relates to an antisense nucleic acid sequence, in particular to an antisense nucleic acid sequence for inhibiting the growth of multidrug-resistant Acinetobacter baumannii. Background technique [0002] Antisense technology is based on the principle of nucleic acid hybridization to design antisense nucleic acids for specific target sequences to inhibit the expression of specific genes. In recent years, its development has been extremely rapid. As a new gene therapy method, antisense technology has been widely used in viral infections, Tumors, genetic diseases and other medical research, and achieved fruitful results, has extremely important theoretical and practical significance. Compared with eukaryotes, prokaryotes have the advantages of simple genome structure and relatively simple growth regulation mechanism. The application of antisense technology to prokaryotic systems undoubtedly has a "magic" application potential. [0003] At pr...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K48/00A61P31/04
Inventor 夏云王慧娟罗鹏
Owner 夏云
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