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A serum-specific metabolite profile of lung cancer patients and its establishment method

A serum and lung cancer technology, applied in the field of lung cancer and metabolomics, can solve the problem of less lung cancer, and achieve the effect of low cost, good application prospect and accurate reflection

Active Publication Date: 2015-12-30
HUZHOU CENT HOSPITAL
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  • Abstract
  • Description
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Problems solved by technology

Metabolomics has a unique prospect in discovering potential biomarkers and improving the early diagnosis of lung cancer. It uses advanced analytical instruments and statistical tools to detect and study markers for a variety of diseases, but reports on the use of metabolomics methods to study lung cancer Relatively small

Method used

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  • A serum-specific metabolite profile of lung cancer patients and its establishment method
  • A serum-specific metabolite profile of lung cancer patients and its establishment method
  • A serum-specific metabolite profile of lung cancer patients and its establishment method

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Embodiment Construction

[0024] The present invention is further described below in conjunction with embodiment.

[0025] 1. Sample collection: collect 5 mL of abdominal blood samples from healthy and normal subjects and lung cancer patients before and 7 days after surgery, put them in sterile coagulation-promoting BD vacuum blood collection tubes, centrifuge at 2500 rpm, 4 ° C for 5 min, take the upper serum, and store in -80 °C refrigerator for use. A total of 30 serum samples were collected in the normal group, and 30 serum samples were collected in each of the lung cancer preoperative and postoperative groups.

[0026] 2 Processing of liquid chromatography-mass spectrometry samples: thaw the cryopreserved serum samples at room temperature and shake well, take 100 μL of samples and add 300 μL of methanol, vortex for 30 s, and stand at 4 °C for 20 min; all samples are refrigerated and centrifuged (12000 rpm, 4 ℃, 15 min), take 200 μL of the supernatant, and transfer it to an injection vial for late...

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Abstract

The invention discloses a serum specificity metabolite spectrum for a patient with a lung cancer and a building method thereof, and particularly discloses a method for carrying out liquid-phase / gas-phase chromatography mass spectrometry metabonomics analysis based on the patient suffering from the lung cancer. The method comprises the following steps: collecting fasting blood samples of a healthy and normal detected person and the patient suffering from the lung cancer 7 days before and after an operation, separating by a chromatogram column after processing; extracting and aligning original data of an instrument after adopting mass spectrometry analysis; building a mathematical model to analyze the metabolites spectrum by adopting multivariable data statistical software; comparing with the difference between an average person with the metabolites spectrum of the patient with the lung cancer, so as to determine the specificity metabolite spectrum. By adopting the method disclosed by the invention, the mutation status of the metabolite of the patient with the lung cancer can be generally and comprehensively reflected, and the serum specificity metabolite spectrum can be applied to early diagnosis of the lung cancer or a beneficial technical support is supplied for prognosis.

Description

technical field [0001] The present invention relates to cancer, especially lung cancer. The present invention also relates to metabolomics, especially metabolomics based on liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry analysis techniques. The invention provides a basis for early diagnosis of lung cancer, and provides specific metabolite profiles and related specific biomarkers in serum of patients with lung cancer before and after operation. Furthermore, the present invention also provides methods for establishing specific metabolite profiles in serum of patients with lung cancer before and after surgery and for screening related specific biomarkers. Background technique [0002] Lung cancer is one of the malignant tumors in the world, ranking first among the causes of cancer death. In the past 20 years, although the research on the mechanism of occurrence and development of lung cancer and the level of diagnosis and treatment have impro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 戴利成陈莹蓉马志红
Owner HUZHOU CENT HOSPITAL
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