A urine biomarker for the early diagnosis of schistosomiasis japonicum and its screening method and application
A biomarker, schistosomiasis technology, applied in the field of biomedicine, can solve the problems of early diagnosis of discomfort, low specificity, low sensitivity, etc., and achieve the effect of accurate reflection and high specificity
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[0036] 1. Establishment of a mouse model of Schistosoma japonicum infection
[0037]Healthy SPF-grade 8-week-old BALB / c mice were divided into seven groups: normal control group, 3-day S. The 28-day infection group and the 42-day Schistosoma japonicum infection group had 10 mice in each group. The normal control group was not treated; the mice in the infection group were infected according to the conventional method: take the positive snails and place them in dechlorinated water, release the cercariae for 1-2 hours under the light condition of 25°C-28°C, and use the inoculation loop to dip in 30 cercariae on the water surface Collected on a coverslip. After the mice were anesthetized, the abdomen was fixed on the mouse board, and the abdomen was shaved and then moistened with dechlorinated water. The coverslips stained with cercariae were attached to the abdominal skin of the mice for 20 minutes to infect.
[0038] 2. Sample collection and pretreatment
[0039] Use metaboli...
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