A urine biomarker for the early diagnosis of schistosomiasis japonicum and its screening method and application

A biomarker, schistosomiasis technology, applied in the field of biomedicine, can solve the problems of early diagnosis of discomfort, low specificity, low sensitivity, etc., and achieve the effect of accurate reflection and high specificity

Active Publication Date: 2021-05-28
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned diagnostic methods have low specificity or low sensitivity, and are not suitable for early diagnosis; therefore, it is necessary to find a method for early diagnosis of schistosomiasis japonicum

Method used

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  • A urine biomarker for the early diagnosis of schistosomiasis japonicum and its screening method and application
  • A urine biomarker for the early diagnosis of schistosomiasis japonicum and its screening method and application
  • A urine biomarker for the early diagnosis of schistosomiasis japonicum and its screening method and application

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Embodiment 1

[0036] 1. Establishment of a mouse model of Schistosoma japonicum infection

[0037]Healthy SPF-grade 8-week-old BALB / c mice were divided into seven groups: normal control group, 3-day S. The 28-day infection group and the 42-day Schistosoma japonicum infection group had 10 mice in each group. The normal control group was not treated; the mice in the infection group were infected according to the conventional method: take the positive snails and place them in dechlorinated water, release the cercariae for 1-2 hours under the light condition of 25°C-28°C, and use the inoculation loop to dip in 30 cercariae on the water surface Collected on a coverslip. After the mice were anesthetized, the abdomen was fixed on the mouse board, and the abdomen was shaved and then moistened with dechlorinated water. The coverslips stained with cercariae were attached to the abdominal skin of the mice for 20 minutes to infect.

[0038] 2. Sample collection and pretreatment

[0039] Use metaboli...

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Abstract

The invention discloses a urine biological marker for early diagnosis of schistosomiasis japonica, wherein the urine biological marker is one or more of xanthonic acid, naphthalenesulfonic acid or heptanoylcarnitine. The sensitivity and specificity of the markers were all greater than 0.9, and the area under the curve (AUC) was greater than 0.9, indicating that the three indicators had good predictive performance and could be used for early diagnosis of schistosomiasis japonica. The invention also discloses a screening method for early diagnosis of schistosomiasis japonica urine biomarkers based on metabolomics. By constructing a mouse schistosomiasis japonica model, the ultra-high performance liquid chromatography-tandem mass spectrometry technology is used to metabolize mouse urine. The omics analysis found and analyzed the characteristic differential metabolites between normal mice and Schistosoma japonicum-infected mice, which were biomarkers for early diagnosis of Schistosomiasis japonicum. The screening method has the characteristics of non-invasiveness, convenience and quickness, and can accurately reflect the difference of the metabolic profile between the mice infected with schistosomiasis japonicum and the normal mice, and has high specificity.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and more specifically, to a urine biomarker for early diagnosis of schistosomiasis japonicum and a screening method and application. Background technique [0002] Schistosoma japonicum is a zoonotic parasitic disease caused by Schistosoma japonicum that seriously endangers human health and hinders social and economic development. It is widely prevalent in Asia, especially in China, the Philippines and Indonesia, where more than 100 Ten thousand people are infected with the disease, and the threatened population is about 46 million people. In my country, Schistosoma japonicum is mainly prevalent in 12 provinces, municipalities and autonomous regions in the middle and lower reaches of the Yangtze River and its south. After more than 60 years of hard work, the prevention and control of schistosomiasis in my country has achieved remarkable results. Now the endemic areas are mainly distributed in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86G01N30/06
CPCG01N30/06G01N30/8686
Inventor 吕志跃胡玥黄萍周洪利马玉斌
Owner SUN YAT SEN UNIV
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