A serum biomarker, screening method and application for early diagnosis of schistosomiasis japonicum

A biomarker, schistosomiasis technology, applied in the field of biomedicine, can solve the problems of early diagnosis of discomfort, low specificity, low sensitivity, etc., and achieve the effect of accurate reflection and high specificity

Active Publication Date: 2021-06-25
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned diagnostic methods have low specificity or low sensitivity, and are not suitable for early diagnosis; therefore, it is necessary to find a method for early diagnosis of schistosomiasis japonicum

Method used

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  • A serum biomarker, screening method and application for early diagnosis of schistosomiasis japonicum
  • A serum biomarker, screening method and application for early diagnosis of schistosomiasis japonicum
  • A serum biomarker, screening method and application for early diagnosis of schistosomiasis japonicum

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Embodiment 1

[0036] 1. Establishment of a mouse model of Schistosoma japonicum infection

[0037]Healthy SPF 8-week-old BALB / c mice were divided into seven groups: normal control group, S. japonicum infection group for 3 days, S. japonicum infection group for 7 days, S. japonicum infection group for 14 days, S. japonicum infection group for 21 days, and S. 28 days of infection group and 42 days of Schistosoma japonicum infection group, 10 mice in each group. The normal control group was left untreated, and the mice in the infected group were infected according to the conventional method: take the positive snails and put them in dechlorinated water, release the cercariae for 1-2 hours under the light condition of 25℃-28℃, and dip 30 cercariae on the water surface with an inoculation loop. Collect on coverslips. After anesthesia, the mice were fixed on the mouse plate with their abdomen facing upwards. The abdomen was shaved and then moistened with dechlorinated water. The cercariae-stained...

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Abstract

The invention discloses a serum biomarker for early diagnosis of schistosomiasis japonicum, and the serum biomarker is phosphatidylcholine and / or palmicholin. The sensitivity and specificity of the markers are both greater than 0.9, and the area under the curve (AUC) is greater than 0.9, indicating that these two indicators have better predictability and can be used for early diagnosis of schistosomiasis japonicum. The invention also discloses a metabolomics-based screening method for serum biomarkers for the early diagnosis of schistosomiasis japonicum, by constructing a mouse schistosomiasis model, and using ultra-high performance liquid chromatography-tandem mass spectrometry to perform metabolomics on mouse serum Analyze, discover and analyze the characteristic differential metabolites between normal mice and Schistosoma japonicum-infected mice, which are biomarkers for early diagnosis of Schistosomiasis japonicum. The screening method has the characteristics of convenience and quickness, can accurately reflect the difference in metabolic spectrum between mice infected with schistosomiasis japonicum and normal mice, and has high specificity.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and more particularly, to a serum biomarker for early diagnosis of schistosomiasis japonica, a screening method and application. Background technique [0002] Schistosomiasis japonicum is a zoonotic parasitic disease caused by Schistosoma japonicum that seriously endangers human health and hinders social and economic development. It is widely prevalent in Asia, especially in China, the Philippines and Indonesia, with more than 100 10,000 people are infected with the disease, and the threatened population is about 46 million. In my country, Schistosoma japonicum is mainly prevalent in the middle and lower reaches of the Yangtze River and 12 provinces, municipalities and autonomous regions in the south. After more than 60 years of efforts, my country has achieved remarkable results in the prevention and control of schistosomiasis. At present, the endemic areas are mainly distributed in Anhui, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86G01N30/06
CPCG01N30/06G01N30/8686
Inventor 吕志跃胡玥陈剑松黄萍周洪利马玉斌
Owner SUN YAT SEN UNIV
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