Application of Fe in relief of Cu toxicity to phaeodactylum tricornutum cells

A technology of Phaeodactylum and tricornutum, applied in the application field of Fe in mitigating the toxicity of Cu to Phaeodactylum tricornutum cells, can solve the problems of ATP content reduction, algae oxygen consumption reduction, respiration inhibition, etc., and achieve obvious mitigation effect , the effect of good application prospects

Inactive Publication Date: 2014-07-23
ZHEJIANG UNIV OF TECH
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Problems solved by technology

It has been reported that high concentrations of copper can lead to a decrease in algae oxygen consumption an

Method used

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  • Application of Fe in relief of Cu toxicity to phaeodactylum tricornutum cells
  • Application of Fe in relief of Cu toxicity to phaeodactylum tricornutum cells

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[0018] Example 1: Application of Fe in alleviating the toxicity of Cu to Phaeodactylum tricornutum cell

[0019] Cultivation of Phaeodactylum tricornutum: Phaeodactylum tricornutum ( Phaeodatylum tricomutum ) Purchased from Wuhan Institute of Hydrobiology, Chinese Academy of Sciences (No. FACHB-863), and cultured using 2X Erdschreiber's medium and Aquatic-4 medium at the same time;

[0020] 1) The medium composition is as follows: KNO 3 1.0g / L, NaCl32g / L, KH 2 PO 4 ·2H 2 O 0.07 g / L, NaHCO 3 0.04g / L, MgCl 2 ·7H 2 O 6.7g / L, MgSO 4 ·7H 2 O 5.5g / L, CaCl 2 ·2H 2 O1.1g / L, A5 solution 1 mL / L, vitamin B1 (Vitamin) 7.5μg / L, vitamin B12 (Vitamin) 10μg / L, biotin (Biotin) 50μg / L, trace metal solution (P-ⅳ Metal Solution ) 12mL / L, NaNO 3 1.98g / L, NaHPO 4 ·7H 2 O 2.39 g / L, the balance is distilled water.

[0021]

[0022] The composition of A5 solution is as follows: H 3 BO 3 0.061 g / L, MnSO 4 ·H 2 O 0.169 g / L,

[0023] ZnSO 4 ·7H 2 O 0.287 g / L, CuSO 4 ·7H 2 O 0.0025 g / L,

[0024] (NH ...

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Abstract

The invention relates to an application of Fe in the relief of Cu toxicity to phaeodactylum tricornutum cells. According to the application, Fe is added into a Cu containing phaeodactylum tricornutum liquid, the concentration of dosed Cu is 0-100 micrograms, the concentration of dosed Fe is 20-80 micrograms, and the amount of cells in all components is continuously determined. According to the application, shown by experiments, Fe basically has no influence on the growth of the phaeodactylum tricornutum cells, the growth of the phaeodactylum tricornutum cells is severely inhibited by Cu, the toxic effect on the phaeodactylum tricornutum cells caused by Cu can be effectively relieved through adding Fe into the Cu containing phaeodactylum tricornutum liquid, and the relief effect is most obvious when 20-80 micrograms of Fe is added into the phaeodactylum tricornutum liquid with the Cu concentration of 0-100 micrograms; and as Fe basically has no influence on the growth of the phaeodactylum tricornutum cells and Fe is ubiquitous and cheap and is easy to get, Fe has a good application prospect in the aspect of relief of the Cu toxicity to the phaeodactylum tricornutum cells.

Description

technical field [0001] The invention relates to the application of Fe in alleviating the toxicity of Cu to Phaeodactylum tricornutum cells. Background technique [0002] With the rapid development of human life and industrialization, more and more heavy metals are discharged into the aquatic environment through fossil fuel incineration, metal smelting, mining, municipal garbage, waste residue, waste water, waste gas, and the application of pesticides and fertilizers, thereby affecting the environment. and serious harm to human health. Over the past few decades, copper has gradually accumulated in aquatic environments due to anthropogenic activities, such as industrial waste disposal, agriculture, drainage of copper mines, and use of copper-based pesticides and fertilizers. It is estimated that annual copper discharges into the aquatic environment have reached 35 to 90×10 3 Ton. According to reports, the average concentration of copper in unpolluted rivers is only 0.25-2 μ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 钱海丰白小翠孙冲冲魏勇傅正伟
Owner ZHEJIANG UNIV OF TECH
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