Identification method for authenticity of varieties of seeds on basis of near-infrared quantitative analysis

A qualitative analysis, authenticity technology, applied in the direction of analyzing materials, material analysis by optical means, measuring devices, etc., can solve problems such as spectral shift

Inactive Publication Date: 2015-02-25
INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the current situation and many shortcomings of the authenticity identification method of seed varieties, and the long-term spectral shift problem with the same instrument, the present invention proposes a method for authenticity identification of seed varieties based on near-infrared qualitative analysis

Method used

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  • Identification method for authenticity of varieties of seeds on basis of near-infrared quantitative analysis
  • Identification method for authenticity of varieties of seeds on basis of near-infrared quantitative analysis
  • Identification method for authenticity of varieties of seeds on basis of near-infrared quantitative analysis

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Embodiment

[0161] The samples of 13 corn varieties used in the experiment were provided by the seed company, all of which have passed the variety approval, namely: H484-248, Jingyu 16, Nonghua 101, Tunyu 24, Tunyu Lvyuan 2102, Tunyu Lvyuan 2104 , Tunyu Lvyuan 2105, Tunyu Lvyuan 2106, Tunyu Lvyuan 2107, Tunyu Lvyuan 2109, Tunyu Lvyuan 2110, Jundan 20, Zhengdan 958. The instrument used is a VECTOR22 / N Fourier transform diffuse reflectance near-infrared spectrometer from BRUKER, Germany. According to the spectrometer setting, the length of each original spectrum is 1114 dimensions, and the spectral range is 3900-12500cm -1 .

[0162] (1) Collect near-infrared spectral data of seeds, and determine the modeling set and test set

[0163] The near-infrared spectral data of the seed samples were collected at different times by a near-infrared spectrometer. The seed samples were all in the shape of complete grains, and the whole cup sampling method was adopted. The experimental data was repeat...

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Abstract

The invention discloses an identification method for the authenticity of the varieties of seeds on the basis of near-infrared quantitative analysis. The method comprises the steps of acquiring near-infrared spectral data of a seed sample and determining a modeling set and testing sets; carrying out pretreatment, partial-least-square feature extraction and linear-identification feature extraction on the modeling set and the testing sets in sequence; selecting a spectral correction set from the modeling set and the testing sets, calculating a transformation matrix between the modeling set and the testing sets; applying the transformation matrix to the remaining testing sets; establishing a quantitative analysis model by adopting a support vector machine method; and carrying out identification on the authenticity of the varieties of the remaining testing sets by utilizing the quantitative analysis model. The identification method disclosed by the invention is established on the basis of near-infrared quantitative analysis and has the advantages that due to a series of operations of spectral pretreatment, feature extraction, correction of the testing sets, modeling and identification and the like, a more stable near-infrared spectral analysis model is established, and the problem of model applicability caused by long-time spectral shift of the same instrument can be solved, so that the identification result is accurate.

Description

Technical field [0001] The field of species of seeds involving seeds, especially a method of authenticity of seed varieties based on near -infrared analysis. Background technique [0002] China is a great agricultural country, and agriculture is a strategic industry that is safe and stable."The country is based on farmers, and farmers are the first". The safety of seed industry is the prerequisite for my country's food safety and one of the prerequisites for agricultural safety.However, my country's species industry market is not optimistic, variety infringement is serious, and fake and inferior seed pit farmers have occurred from time to time.Enterprises have difficulty in protecting their rights, difficult litigation, and difficulty in chasing claims, causing many excellent varieties to be infringed, so that those who form breeding are not as good as selling species and scientific research. [0003] At present, the variety identification methods can be divided into the followin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359
Inventor 张丽萍李卫军董肖莉覃鸿
Owner INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
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