Application of basil polysaccharide as paclitaxel synergist in the preparation of antitumor drugs

An anti-tumor drug, basil polysaccharide technology, applied in the direction of anti-tumor drugs, drug combinations, medical preparations containing active ingredients, etc., can solve problems such as enhancement, prolong survival rate, increase tumor growth inhibition, and improve average survival effect of time

Active Publication Date: 2017-04-12
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The role of basil polysaccharides in anti-tumor metastasis has been reported in the literature, and it can be used for a variety of tumor metastasis. However, no reports have shown that basil polysaccharides can enhance the efficacy of the chemotherapy drug paclitaxel in treating tumors.

Method used

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  • Application of basil polysaccharide as paclitaxel synergist in the preparation of antitumor drugs
  • Application of basil polysaccharide as paclitaxel synergist in the preparation of antitumor drugs
  • Application of basil polysaccharide as paclitaxel synergist in the preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Effects of Basil Polysaccharide on Three Kinds of Tumor Cells

[0022] Experimental materials: basil polysaccharide is the finished product of basil polysaccharide obtained by the extraction and separation method provided by the present invention; human lung cancer cell line A549 and human laryngeal cancer cell line Hep -2 and human gastric cancer cell line SGC-7901.

[0023] Experimental method: Dissolve the finished basil polysaccharide in an appropriate amount of deionized water to prepare a solution with a concentration of 10 mg / ml, sterilize it, subpackage it and store it in a -20°C refrigerator for later use. Human lung cancer cell line A549, human laryngeal cancer cell line Hep-2 and human gastric cancer cell line SGC-7901 were routinely cultured in RPMI1640 medium containing 10% newborn bovine serum (NBS), at 37°C, 5% CO2, and saturated humidity cultured in a cell culture incubator. Digest and passage with 0.25% trypsin. Take the cultured logarithmic growth p...

Embodiment 2

[0028] In Vitro Pharmacodynamic Study on the Synergistic Effect of Basil Polysaccharide on Paclitaxel

[0029] Experimental materials: except adding paclitaxel injection (specification: 30mg / 5ml, Beijing Concord Pharmaceutical Factory), the others are the same as in Example 1.

[0030] Experimental content: Except that paclitaxel injection (specification: 30mg / 5ml, Beijing Xiehe Pharmaceutical Factory) was prepared according to the instructions and used as the drug to be tested together with basil polysaccharide, the others were the same as in Example 1. The experimental results are shown in Table 1.2.

[0031] Table 1.2 Synergistic effect of basil polysaccharides on paclitaxel in vitro tumor inhibition

[0032]

[0033] Note: *Compared with paclitaxel 1.25μg / ml group, p<0.01; △compared with paclitaxel 1.25μg / ml group, p<0.05.

[0034] The above experimental results show that when the chemotherapy drug paclitaxel is used alone, paclitaxel has certain tumor growth inhibito...

Embodiment 3

[0036] In vivo pharmacodynamics study on the synergistic effect of basil polysaccharides on paclitaxel

[0037] H22 tumor-bearing mice experiments

[0038] Kunming male mice (body weight 18-22 g) were adaptively fed for 3 days. Aseptically extract the ascites of ascites-type H22 liver cancer subcultured mice, dilute the cell suspension with normal saline to adjust the cell density to 5×106 / ml, and inoculate it subcutaneously in the axilla of the right forelimb of the mouse, injecting 0.2ml per mouse.

[0039] Experimental grouping and administration: On the second day after the model was replicated, the mice were randomly divided into 5 groups, namely: ① normal saline group as negative control group; ② paclitaxel 10 mg / kg group; ③ paclitaxel 10 mg / kg + basil polysaccharide Dose group (25mg / kg); ④ paclitaxel 10mg / kg + ocsil polysaccharide medium dose group (50mg / kg); ⑤ paclitaxel 10mg / kg + ocsil polysaccharide high dose group (100mg / kg).

[0040] Experimental method: From the...

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Abstract

The invention discloses novel application of the pure traditional Chinese medicine preparation basil polysaccharide, namely application of basil polysaccharide as taxol synergist in preparing antineoplastic drugs. As proved by research findings, combination of basil polysaccharide and taxol has the functions of inhibiting proliferation and activity of tumour cells (including lung cancer, laryngocarcinoma, stomach cancer and the like) from different tissue sources; in addition, according to a basil polysaccharide extracorporal cell experiment, the working concentration is free of toxicity, and basil polysaccharide is dose-dependent on the synergism of the taxol and has a clinical application prospect.

Description

technical field [0001] The invention specifically relates to the new application of basil polysaccharide in preparing anti-tumor paclitaxel synergist, that is, the application of basil polysaccharide as paclitaxel synergist in the preparation of anti-tumor drugs, which belongs to the technical field of synergistic effect of traditional Chinese medicine on tumor chemotherapy drugs. Background technique [0002] Tumor is a disease that seriously endangers human health and life. Chemotherapy is a systemic treatment that uses chemical drugs to kill cancer cells, and is widely used in the clinical treatment of cancer patients. Paclitaxel is a new type of anti-microtubule chemotherapeutic drug. It promotes the polymerization of tubulin, inhibits depolymerization, maintains the stability of tubulin, and hinders cell mitosis, thereby inhibiting tumor growth. Paclitaxel has special effects on breast cancer, ovarian cancer, uterine cancer, lung cancer, gastric cancer, etc. However, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P35/00A61K31/337
CPCA61K31/337A61K31/715A61K2300/00
Inventor 朱庆均张丹肖丽周洪雷于华静李路
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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