Unlock instant, AI-driven research and patent intelligence for your innovation.

Human cyclooxygenase-2

A cyclooxygenase, human technology, applied in the field of medicine

Inactive Publication Date: 2015-04-15
SHANGHAI MANYI TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thus, selective inhibitors of cyclooxygenase-2 would have anti-inflammatory, antipyretic and analgesic properties similar to conventional nonsteroidal anti-inflammatory drugs (NSAIDs), would inhibit hormone-induced uterine contractions, would have Potential anticancer effects, but will also have reduced ability to induce some mechanism-based side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

no. 2 example

[0016] In a second embodiment, the present invention includes a composition comprising (a) a preparation of osteosarcoma cells having about 103, about 109 osteosarcoma cells per milliliter of the cell preparation, and (b) 0.1? between 50. mu.l of peroxide-free arachidonic acid per ml of cell preparation.

[0017] Typically, the preparation of cells will be grown as a monolayer, using an aliquot of cells per well (approximately 1 ml working volume) as in 8.2.times.10.sup.4 2.times.10.sup.6 The protocol described below. Arachidonic acid is typically used in amounts of 1 to 20 μl per well with a working volume of approximately 1 ml.

[0018] When using osteosarcoma microparticles, rather than whole cells, cell preparations generally contain 50 to 500 micrograms per milliliter of cell-prepared microsomal protein. Arachidonic acid is usually used in amounts of 1 to 20. Prepare mu.l acid per ml of cells.

no. 3 example

[0019] In the third embodiment, the present invention includes a sample for determining the detected cyclooxygenase-1 activity, comprising the following steps: (a) preparing the cells of (1), cells expressing cyclooxygenase-1, but Not expressing COX-2, (2) samples including a putative cyclooxygenase-1 inhibitor, (3) arachidonic acid, and (b) determination of the amount of PGE produced in step (a) .sub.2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving expression of human cyclooxygenase-2 in a transformed cell and detecting human cyclooxygenase-1 in which cDAN of human cyclooxygenase-2 that is composed of preferential and independent DNA is connected with 3' flanking sequence. The invention further provides a sample for measuring cyclogenase-2.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a human cyclooxygenase-2. Background of the invention [0002] The present invention includes a human cyclooxygenase-2 protein including high-level expression of human high-expression cyclooxygenase-2 (COX-2) cDNA and mammalian expression vector system. [0003] Exerts the anti-inflammatory, analgesic, and antipyretic activities of NSAIDs, and inhibits hormone-induced uterine contractions and the growth of certain types of cancer, by inhibiting prostaglandin G / H synthase, also known as cyclooxygenation enzyme. Until recently, only one form of cyclooxygenase was characterized, and this corresponds to cyclooxygenase-1 (COX-1), the constituent enyme originally identified in bovine seminal vesicles. Recently the gene for an inducible form of cyclooxygenase (cyclooxygenase-2; COX-2)) has been cloned, sequenced, and characterized from chicken, mouse, and human sources. cyclooxygnase-2...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02
CPCC12N9/0083C12Y114/99001
Inventor 陈晴川
Owner SHANGHAI MANYI TECH