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Bacillus subtilis jn005 and its application in controlling rice blast

A technology for Bacillus subtilis and rice blast, applied in application, bacteria, fungicides and other directions, can solve the problems of reduced control effect of antagonistic microorganisms, unsatisfactory effect, poor colonization ability of antagonistic microorganisms, etc., and achieves good control effect and protection. Combined with the therapeutic effect, the effect of avoiding adverse effects

Active Publication Date: 2018-10-02
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the field of biological control, there are often problems such as poor colonization ability of antagonistic microorganisms under natural conditions in the field and unstable effect on disease control.
A large number of studies have also shown that the antagonistic microorganisms screened indoors have a strong inhibitory effect on pathogenic bacteria in the greenhouse, but the effect in field control is not satisfactory. Colonize the root circle of plants, on the other hand, interact with the complex microbial species and antagonistic strains in the field soil, resulting in a decrease in the control effect of antagonistic microorganisms. Therefore, it is necessary to select and screen biological strains with strong field adaptability or high-efficiency metabolites Applying to field disease control is an important way to solve the above problems

Method used

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  • Bacillus subtilis jn005 and its application in controlling rice blast
  • Bacillus subtilis jn005 and its application in controlling rice blast
  • Bacillus subtilis jn005 and its application in controlling rice blast

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1 The separation and acquisition of Bacillus subtilis JN005

[0025] Healthy rice plants were selected from the diseased clusters of rice blast-susceptible rice variety Xiangzaoxian 24 in Paixingshang Village (N28°31′49.02″, E111°54′24.86″), Daligang Town, Taojiang County, Hunan Province, and a total of 871 plants were isolated For strains, an antagonistic bacteria JN005 with an inhibitory rate of 84% against Magnaporthe oryzae mycelium was screened out by using plate confrontation method with Magnaporthe oryzae.

[0026] Bacterial strain JN005 ( figure 2 A and figure 2 B), observe the colony morphology, observe the scanning electron microscope images of the bacteria, and combine the physiological and biochemical and microbiological detection results of the bacteria, and the 16S rDNA sequencing results (see SEQ ID NO.1), to finally determine the bacterial strain JN005 is Bacillus subtilis.

[0027] In addition, the plate confrontation culture method was ...

Embodiment 2

[0028] Example 2 Application of Bacillus subtilis JN005 in the Control of Rice Blast

[0029] After the bacterial strain JN005 was activated on the NA solid medium plate, two bacterial cakes with a diameter of 5mm were punched out and inserted into 150mL NB liquid medium (peptone 5g / L, beef extract 3g / L, glucose 20g / L L, pH 7.0, prepared with distilled water) in a 250mL Erlenmeyer flask, cultured in a shaking table at 28°C and 180r / min for 6 days, and filtered with a bacterial filter to obtain the metabolite of Bacillus subtilis JN005.

[0030]Add 10mL of metabolites of strain JN005 and 0.1mL of conidium liquid of Magnaporthe grisea (strain No. 81) into a sterilized Erlenmeyer flask, mix well, replace antagonistic bacteria culture solution with sterile water as a control, and incubate in the dark at 27°C for 6h Microscopic examination of spore germination, the experiment was repeated 3 times. The results showed that the inhibition rate of JN005 metabolites on the spore germin...

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Abstract

The invention discloses a strain of Bacillus subtilis JN005 and its application in preventing and treating rice blast. The preservation number of the strain JN005 is CCTCC No: M2015228. The use of strain JN005 to control rice blast avoids the potential adverse effects of chemical control on the environment and rice safety and the risk of infection caused by the use of disease-resistant varieties. The live bacteria of strain JN005 and its metabolites have a good preventive effect on rice blast, and have both protective and therapeutic effects. It can be processed and developed as a live bacterial agent and metabolite formulation. In addition, strain JN005 has inhibitory effect on various pathogenic indicator bacteria.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a bacillus subtilis JN005 and its application in preventing and treating rice blast. Background technique [0002] Rice (Oryza sativa) is one of the most important food crops, and more than half of the world's population feeds on rice. Rice blast (the pathogen is Magnaporthe oryzae, and its asexual generation is Pyiicularia oryzae) is one of the most serious fungal diseases of rice. M. oryzae is a semi-living parasite with a distinct biotrophic phase during the initial stages of infection. Rice blast can be divided into seedling blast, leaf blast, knot blast, panicle blast, grain blast, and branch blast according to the location and time of onset. At present, the production mainly uses disease-resistant varieties and chemical fungicides to control rice blast. The main fungicides used are tricyclazole, rice blastling, prochloraz, rice blastamide, isoframine, rice bla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/125
CPCA01N63/00A01N63/10C12N1/205C12R2001/125
Inventor 刘二明任佐华管玲莉刘敏捷周鑫钰
Owner HUNAN AGRICULTURAL UNIV