DNA bar code standard gene used for identifying culex tritaeniorhynchus and applications

Abstract

The invention belongs to the biotechnology field, and especially relates to a DNA bar code standard gene used for identifying culex tritaeniorhynchus and applications. The nucleotide sequence of the DNA bar code standard gene used for identifying culex tritaeniorhynchus is shown in the ID No.1 in a sequence table. The provided DNA bar code standard gene can be applied to identification whether a specimen to be detected is culex tritaeniorhynchus. The DNA bar code standard gene is suitable for practical needs of border port health quarantine work, and a relatively stable and accurate molecule identification method is established by utilization of the DNA bar code technology and aimed to culex tritaeniorhynchus. Defected samples can be identified, culex tritaeniorhynchus and sibling species can be distinguished and identified well, and the non-imago phase of culex tritaeniorhynchus can be identified accurately. The provided DNA bar code standard gene has important promoting effects on raising accuracy of culex tritaeniorhynchus classification identification and promoting health supervision work quality improvement.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a DNA barcode standard gene for identifying Culex tritaeniorhynchus and its application. Background technique [0002] At present, the classification and identification of vector organisms at home and abroad mainly rely on traditional morphological classification methods. This method is mainly aimed at the adult stage, and is limited to vectors of other developmental stages (larvae, pupae, etc.) captured in the actual work of health supervision; for the identification of closely related species of vectors with similar external shapes, the accuracy of the morphological classification method In addition, incomplete samples are often collected in practical work, and morphological methods encounter many difficulties in the identification of incomplete samples. In the port quarantine work, if the intercepted larvae, eggs, pupae and other non-adult worms, it is usually necessary to cultiv...

AI Technical Summary

Problems solved by technology

This method is mainly aimed at the adult stage, and is limited to other developmental stage vectors (larvae, pupae, etc.) captured in the actual work of health supervision; for the identification of closely related species of vector species similar to Culex tritaeniorhynchus in external form, The accuracy of the morphological classification method is low

In the actual work of inspection and quarantine, non-adult insects such as larvae, eggs, and pupae are often intercepted. Usually, they need to be cultivated into adults before identification. The entire quarantine identification process takes several weeks, and often collected Incomplete samples, morphological methods encounter many difficulties in the identification of incomplete samples, which greatly lengthens the customs clearance period

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