Method for investigation of liver damage type

Abstract

Provided is an investigation method for determining liver damage type, using as an indicator the concentration of lithocholic acid (hereinafter abbreviated as LCA) in a biological sample derived from a test subject. Specifically, there is provided an investigation method in which the concentration of LCA in a biological sample taken from a test subject is measured, and using the measured LCA concentration as an indicator, the type of liver damage is determined to be hematocyte damage type, cholestatic type, or a mixed type thereof.

Description

technical field

[0001] The invention relates to a method for examining liver damage, in particular to a method for judging the type of liver damage. More specifically, the present invention relates to a test method for determining the type of liver damage, wherein the concentration of LCA in a biological sample collected from a test subject is measured, and the measured concentration of LCA is used as an index. Background technique

[0002] The liver is a glandular tissue attached to the digestive tract. It has the functions of bile production and secretion, detoxification, glucose metabolism, protein metabolism, production of blood coagulation factors, hormone regulation, and various biological components such as fat, glycogen, protein, and vitamins. Storage and other important functions for living organisms. Therefore, when these functions are acutely or chronically injured by viral infection, excessive intake of drugs or poisons, alcohol, etc., the maintenance of the hom...

Examples

Embodiment 1

[0110] For the purpose of developing biomarkers useful for the early diagnosis of liver damage types, the concentrations of bile acid components, oxidative stress markers, and liver fibrin markers were measured using serum samples obtained from patients with liver damage and healthy volunteers . The collection of serum samples and a series of experiments were carried out with the consent of the patients and healthy volunteers as the sample providers.

[0111] 1. Target sample

[0112] Based on the approval of Daiichi Sankyo Co., Ltd. and the ethics committee of Jihui Medical University, serum was collected from 150 patients suspected of having viral, autoimmune, alcoholic, genetic, etc. liver damage within about 2 years Sample 304 sample. In addition, blood collected from volunteers was used as a serum sample derived from a healthy person. Serum samples were stored at -80°C prior to biomarker determination.

[0113] 2. Evaluation items

[0114] Determination of various bi...

technical field

[0001] The invention relates to a method for examining liver damage, in particular to a method for judging the type of liver damage. More specifically, the present invention relates to a test method for determining the type of liver damage, wherein the concentration of LCA in a biological sample collected from a test subject is measured, and the measured concentration of LCA is used as an index. Background technique

[0002] The liver is a glandular tissue attached to the digestive tract. It has the functions of bile production and secretion, detoxification, glucose metabolism, protein metabolism, production of blood coagulation factors, hormone regulation, and various biological components such as fat, glycogen, protein, and vitamins. Storage and other important functions for living organisms. Therefore, when these functions are acutely or chronically injured by viral infection, excessive intake of drugs or poisons, alcohol, etc., the maintenance of the hom...

Examples

Embodiment 1

[0110] For the purpose of developing biomarkers useful for the early diagnosis of liver damage types, the concentrations of bile acid components, oxidative stress markers, and liver fibrin markers were measured using serum samples obtained from patients with liver damage and healthy volunteers . The collection of serum samples and a series of experiments were carried out with the consent of the patients and healthy volunteers as the sample providers.

[0111] 1. Target sample

[0112] Based on the approval of Daiichi Sankyo Co., Ltd. and the ethics committee of Jihui Medical University, serum was collected from 150 patients suspected of having viral, autoimmune, alcoholic, genetic, etc. liver damage within about 2 years Sample 304 sample. In addition, blood collected from volunteers was used as a serum sample derived from a healthy person. Serum samples were stored at -80°C prior to biomarker determination.

[0113] 2. Evaluation items

[0114] Determination of various bi...

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