Chromogenic substrate of 3-nitro-4-chlorine-benzenesulfonamide glutathione-S-transsulfurase

Abstract

The invention discloses a chromogenic substrate of 3-nitro-4-chlorine-benzenesulfonamide glutathione-S-transsulfurase. The chromogenic substrate is structurally characterized in that 3-nitro-4-chlorine-benzene sulfonyl chloride and one of ethanolamine, aminoethyl sulfonic acid, glycine and N,N-dimethyl-ethylenediamine are reacted to generate benzenesulfonamide; under catalysis of glutathione-S-transsulfurase (GST), the chromogenic substrate and reduced glutathione (GSH) are reacted to generate phenyl sulfide, the absorbing effect of the obtained phenyl-sulfide product is remarkably enhanced relative to the absorbing effect of the chromogenic substrate near 285 nm; the initial absorbing variation velocity near 285 nm in the process that the chromogenic substrate and the GSH are reacted under the analysis of the GST is measured, and can express the activity of the GST and be used for screening inhibitors.

Description

technical field [0001] The invention belongs to the field of enzyme biotechnology, and relates to a chromogenic substrate for measuring the activity of glutathione-S-transsulfurase (GST) by tracking absorption changes; this type of substrate corresponds to the reaction of glutathione GSH under the action of GST The phenylene sulfide in which the 4-position chlorine atom on the benzene ring is replaced by the sulfur atom in GSH is convenient for determining enzyme activity and screening GST inhibitors by measuring product absorption. Background technique [0002] Glutathione sulfur transsulfase (GST) is a group of multifunctional isoenzymes widely distributed in various organisms. Its main function is to catalyze the electrophilic group and reduced form of some endogenous or foreign harmful substances. The sulfhydryl coupling of glutathione generates adducts, reduces its chemical reactivity, and promotes the coupled adducts to be excreted from cells, avoiding toxicity to cell...

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However, CDNB is too poorly soluble in wate

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