A bovine serum albumin detection probe and its preparation method and application

A technology for bovine serum albumin and detection probes, applied in the field of bovine serum albumin detection probes and its preparation, can solve the problems of harsh detection conditions, cumbersome detection steps, high toxicity of reagents, etc., and achieve low detection cost and excellent preparation method Simple, Widely Applicable Effects
CN106324260BInactive Publication Date: 2018-06-22CHENGDU INST OF BIOLOGY CHINESE ACAD OF S +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Publication Date
2018-06-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a bovine serum albumin detection probe and its preparation method and application. The preparation method uses cheap tetraethyl orthosilicate as a raw material, and the tetraethyl orthosilicate is hydrolyzed under the action of ammonia water to synthesize nano Grade silica particles; then use 3-aminopropyltriethoxysilane to bond amino groups on the surface of silica particles to obtain NH2-SiO2; then bond BSA to NH2-SiO2 to obtain BSA -SiO2. The preparation method is simple, the reaction conditions are mild, easy to realize, and suitable for large-scale industrial production. The detection method using BSA-SiO2 to detect BSA utilizes the specific binding reaction between antigen and antibody, and at the same time uses HRP to label the antibody to achieve visual detection, with strong specificity and high sensitivity.
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Description

technical field

[0001] The invention relates to the field of immune analysis, in particular to a detection probe for bovine serum albumin and its preparation method and application. Background technique

[0002] In the process of culturing cells to produce vaccines, bovine serum is the main component of the medium. Since bovine serum albumin is often contained in bovine serum, it is very important to detect residual bovine serum albumin (BSA) in vaccines, which is directly related to the safety of biological products. However, the current detection methods all require expensive instruments, and at the same time, the detection steps are cumbersome, time-consuming, and the detection conditions are harsh, and the reagents used in the detection are highly toxic. Contents of the invention

[0003] The first object of the present invention is to provide a bovine serum albumin detection probe, which has a small particle size, stable structure and easy preservation. The bovine s...

Claims

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