Check patentability & draft patents in minutes with Patsnap Eureka AI!

A method for reducing quantification errors caused by an optical artefact in digital polymerase chain reaction

A technology of chain reaction and quantification error, applied in biochemical equipment and methods, material analysis by optical means, measurement device, etc., can solve the problem of not considering quantification error, etc.

Pending Publication Date: 2018-08-17
F HOFFMANN LA ROCHE & CO AG
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, prior art methods do not account for quantification errors in PCR due to situations in which optical assays are compromised by artifacts

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for reducing quantification errors caused by an optical artefact in digital polymerase chain reaction
  • A method for reducing quantification errors caused by an optical artefact in digital polymerase chain reaction
  • A method for reducing quantification errors caused by an optical artefact in digital polymerase chain reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] In a first aspect, the present invention relates to a method for reducing quantification errors caused by optical artifacts in a digital polymerase chain reaction (dPCR), wherein the amount or concentration of a nucleic acid of interest is quantified in an array of reaction regions , the method consists of

[0009] a) providing an array of reaction regions used in dPCR;

[0010] b) determining the distribution of the optical signal in each reaction zone;

[0011] c) if the optical signal in the reaction region determined in step b) is not uniformly distributed in the reaction region

[0012] If not, identify the reaction zone as invalid; and

[0013] d) Eliminating reaction regions identified as invalid from the calculation of the amount or concentration of the nucleic acid of interest.

[0014] As detailed above, methods for the reliable determination of the amount or concentration of nucleic acids are of particular importance in several industrial applications, for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method for reducing quantification errors caused by an optical artefact in digital polymerase chain reaction (dPCR) and to a method for determining the amount or concentration of a nucleic acid of interest in a sample with dPCR.

Description

field of invention [0001] The present invention relates to a method for reducing quantification errors caused by optical artifacts in digital polymerase chain reaction (dPCR) and a method for determining the amount or concentration of a nucleic acid of interest in a sample with dPCR. Background of the invention [0002] For many biological, biochemical, diagnostic or therapeutic purposes, it is necessary to accurately and precisely determine the amount or concentration of nucleic acid in a sample. dPCR is a newer approach to nucleic acid detection and quantification that provides an alternative to routine real-time quantitative PCR for absolute quantification of nucleic acids and detection of rare alleles. dPCR works by distributing a sample of nucleic acid into many separate, parallel PCR reactions; some of these reactions contain (positive) and some do not (negative) the target molecule. Following PCR analysis, the fraction of negative reactions is used to generate an abs...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/686
CPCC12Q1/686C12Q2565/629C12Q2565/631C12Q1/6851G01N2021/6441C12Q2537/165C12Q2563/159C12Q2565/607
Inventor P·瓦泽F·贝特沙尔特
Owner F HOFFMANN LA ROCHE & CO AG
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com