Gene Mutation Detection Methods

Abstract

The present invention relates to the field of molecular biology, and provides a gene mutation detection method, which includes preparing a sequencing reaction system for a sample to be sequenced, and the sequencing reaction system includes a template strand to be tested and a sequencing primer connected at one end to the same solid phase carrier chain; the sequencing primer chain includes a sequencing primer sequence and a sequencing junction sequence; the sequencing junction sequence is located at the fixed end of the sequencing primer chain, and the sequencing primer sequence is located at the free end of the sequencing primer chain, as the The sequencing primer of the test template chain; the sequence of the sequencing primer is anchored on the test template chain, and the gene detection is performed on the site to be sequenced on the test template chain. The gene mutation detection method of the present invention improves the sequencing efficiency; when multiple samples to be sequenced are sequenced in the same system at the same time, there is no need to add additional sequencing primers, avoiding mutual interference caused by different sequencing primers, and improving the sequencing efficiency and accuracy.

Description

technical field [0001] The invention relates to the field of molecular biology, more specifically, to a gene mutation detection method. Background technique [0002] Gene mutation is related to the replication of deoxyribonucleic acid, DNA damage repair, cancer and aging. Gene mutation is also one of the important factors of biological evolution. Therefore, the study of gene mutation has a wide range of biological significance in addition to its theoretical significance. [0003] For the detection of gene mutations, the second-generation high-throughput sequencing technology has been widely used due to its accuracy and sensitivity, and has been involved in various aspects of life science research and medical research. Mutation detection is also one of the current research hotspots. However, in the gene mutation detection method based on the second-generation high-throughput sequencing technology, when multiple samples to be sequenced are detected at the same time, a variety...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a gene mutation detection method, which aims to solve the problem of mutual interference between different sequencing primers when multiple sites to be sequenced are detected in the same system at the same time in the prior art

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