A tissue fixation and exfoliation agent for tissue blocks and its application

A technology of tissue fixation and tissue block, applied in application, preservation of human or animal bodies, animal husbandry, etc., can solve the problems of not being able to fully observe all types of cells, only two-dimensionality, complicated process, etc., and achieve rapid shedding , high accuracy, simple and fast method

Active Publication Date: 2019-04-05
济南天灿医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the current stage, the tissue is sliced ​​and then observed under a microscope. However, due to the complexity of the slicing process and the two-dimensional nature, all types of cells cannot be completely observed; It is difficult for the cells to fall off from the surface of the tissue block, and it is difficult to collect a single large number of exfoliated cells from the formaldehyde-fixed tissue block for cytological research.

Method used

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  • A tissue fixation and exfoliation agent for tissue blocks and its application

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Embodiment 1

[0018] A tissue fixation and exfoliation agent for tissue blocks, consisting of the following raw materials in weight percentage: 10% formaldehyde; 0.1% EDTA; 15% methanol; 1% poloxamer; the rest is purified water, adjusted by phosphate buffer The pH is 7.0.

[0019] For the application of the above reagents, put fresh tissue pieces obtained from muscles into the prepared reagent bottle, and let it stand for 20 minutes to obtain the fixed tissue pieces, and then vibrate in a 1200-rpm oscillator for 15 minutes to obtain cell suspension.

Embodiment 2

[0021] A tissue fixing and exfoliating agent for tissue blocks is composed of the following raw materials in weight percentage: 15% formaldehyde; 1% EDTA; 5% methanol; 2% PEG400;

[0022] For the application of the above reagents, put the fresh tissue pieces obtained from the stomach into the prepared reagent bottle, and let it stand for 25 minutes to obtain the fixed tissue pieces, and then vibrate in an oscillator at 1800 rpm for 15 minutes to obtain cell suspension.

Embodiment 3

[0024] A tissue fixing and exfoliating agent for tissue blocks is composed of the following raw materials in weight percentage: 10% formaldehyde; 0.5% EDTA; 10% methanol; the rest is pure water, and the pH of the phosphate buffer is adjusted to 7.2.

[0025] For the application of the above reagents, put fresh tissue pieces obtained from the intestine into the prepared reagent bottle, and let it stand for 30 minutes to obtain the fixed tissue pieces, then vibrate in a 1500-rpm oscillator for 15 minutes, and then carry out The pellets were prepared by centrifugation to obtain the cell suspension.

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Abstract

The invention provides a tissue fixing and shedding agent for a tissue block and aims at the problem in the process of existing istocytology analysis. The tissue fixing and shedding agent is composedof, by weight, 10-15% of formaldehyde, 0.1-1% of EDTA, 5-15% of methanol, 0-2% of PFG400, 0-1% of and the balance of purified water, and pH is 7.0-7.5. In addition, the invention further provides application of the agent. The tissue fixing and shedding agent can shed cells on tissue blocks after tissue fixing, and double effects of fixing tissue and extracting cell suspensions are realized. A process of the tissue fixing and shedding agent is simple and easy to implement, and cancer cells can be detected accurately and quickly.

Description

technical field [0001] The invention relates to the field of tissue fixation liquid and cell exfoliation agent, in particular to a tissue fixation exfoliation agent for tissue blocks and its application. Background technique [0002] At present, after animal and human tissues are removed, in order to prevent tissue corruption, mutation and biological contamination, and prevent the spread of germs and viruses, the principle is to directly put them in 10% formaldehyde for fixation, and then perform post-section, staining, research and other work. For histological studies. At the current stage, the tissue is sliced ​​and then observed under a microscope. However, due to the complexity of the slicing process and the two-dimensional nature, all types of cells cannot be completely observed; The cells are difficult to come off from the surface of the tissue block, and it is difficult to collect a single large number of exfoliated cells from the formaldehyde-fixed tissue block for ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 李富亮张震
Owner 济南天灿医疗科技有限公司
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