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Plant seed fatty acid-related protein ghbzip67 and its encoding gene and application

A fatty acid and plant technology, applied in plant genetic improvement, botanical equipment and methods, applications, etc., can solve the difficulties of cloning oil-related genes, slow progress in the mapping of cottonseed oil-related genes, relatively few studies on cottonseed oil content, etc.

Active Publication Date: 2021-08-10
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The mapping of cottonseed oil content-related genes progressed slowly. Domestic and foreign researchers mainly focused on QTLs for fiber quality-related traits and QTLs for yield-related traits. Due to the limitation of molecular marker density, it is still very difficult to clone oil-related genes by forward genetics map-based cloning

Method used

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  • Plant seed fatty acid-related protein ghbzip67 and its encoding gene and application
  • Plant seed fatty acid-related protein ghbzip67 and its encoding gene and application
  • Plant seed fatty acid-related protein ghbzip67 and its encoding gene and application

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Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1, the acquisition of GhBZIP67 protein and its coding gene

[0040]The total RNA of different tissue and organ materials of cotton land-sea backcross inbred line 3008 was extracted and reverse transcribed into cDNA. After a large number of sequence analysis, expression analysis and functional verification, a DNA coding sequence was found from the cDNA, as shown in sequence 2 of the sequence listing, and its encoded protein was shown in sequence 1 of the sequence listing.

[0041] The protein shown in Sequence 1 of the Sequence Listing was named GhBZIP67 protein. The gene encoding GhBZIP67 protein was named as GhBZIP67 gene.

Embodiment 2

[0042] Example 2, Functional verification of GhBZIP67 protein and its coding gene

[0043] 1. Obtaining of transgenic plants

[0044] 1. Construction of the recombinant expression vector: The fragment between the BamHI and SacI restriction sites of the pBI121 vector was replaced with the DNA molecule shown in the sequence 2 of the sequence listing from the 1-1176 position of the 5' end to obtain the recombinant expression vector pBI121: : GhBZIP67 (sequencing verification).

[0045] 2. The recombinant expression vector pBI121::GhBZIP67 obtained in step 1 was introduced into Agrobacterium GV3101 to obtain the recombinant strain GV3101::GhBZIP67.

[0046] 3. Transfer the recombinant strain GV3101::GhBZIP67 obtained in step 2 into Arabidopsis Col-0 by the flower dipping method. The specific transformation steps are as follows:

[0047] (1) Inoculate the recombinant bacteria GV3101::GhBZIP67 obtained in step 2 into YEB liquid medium containing 100mg / L rifampicin, 25mg / L Genta an...

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Abstract

The invention discloses a plant seed fatty acid related protein GhbZIP67, its encoding gene and application. The protein provided by the present invention is the following (a1) or (a2): (a1) a protein composed of the amino acid sequence shown in sequence 1 in the sequence listing; (a2) the amino acid sequence shown in sequence 1 in the sequence listing after a A protein derived from Sequence 1 that has substitution and / or deletion and / or addition of several amino acid residues and is related to plant seed oil. The invention provides GhbZIP67 protein and its coding gene, which can regulate the fatty acid content of plant seeds. The invention has important significance for cultivating new cotton varieties and is suitable for popularization and application.

Description

technical field [0001] The invention relates to a plant seed fatty acid related protein GhbZIP67 and its encoding gene and application. Background technique [0002] Cotton is an important fiber crop as well as an important oil crop. In the cotton kernel of upland cotton, oil accounts for about 25%-40%, and its oil content is equivalent to that of soybean. It is an important source of vegetable oil, and cotton ranks sixth among oil crops in the world. Researchers' research on upland cotton mainly focuses on fiber quality, yield traits and resistance traits, and less research on cottonseed oil content traits. Upland cotton is planted in a wide area, and about 1.65 kilograms of cottonseed is produced for every kilogram of fiber produced in production. A large amount of cottonseed can be produced in the world every year, and upland cottonseed can be used as an important source of oil. With the growth of population, the demand for edible vegetable oil is increasing, and the pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/10A01H6/20
CPCC07K14/415C12N15/8247
Inventor 臧新山裴文锋吴嫚于霁雯李兴丽张金发
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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