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Soybean lipid transporter gene GmABCA9 and application thereof

A technology of soybeans and genes, applied in the field of genetic engineering

Active Publication Date: 2019-03-22
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the transport system of oil between chloroplast and endoplasmic reticulum, and there is no report in soybean

Method used

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  • Soybean lipid transporter gene GmABCA9 and application thereof
  • Soybean lipid transporter gene GmABCA9 and application thereof
  • Soybean lipid transporter gene GmABCA9 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] Cloning of GmABCA9 gene

[0030] Using the RNA of soybean Heinong 44 variety as a template, after synthesizing cDNA, it was amplified by PCR, and the amplification primer pair was as follows:

[0031] 5'-TGCTCTAGAATGGCAACCACTCTCACC-3'

[0032] 5'-TGCTCTAGACCACTGAAAAGCTACTGAACT-3',

[0033] A gene sequence of 2904bp was obtained, as shown in SEQ ID No.1, named soybean GmABCA9 gene; a total of 967 amino acids were encoded, as shown in SEQ ID No.2.

[0034] Expression Analysis of GmABCA9 in Soybean Tissue

[0035] The temporal and spatial expression of genes provides an important basis for the verification of the function of the target gene to a certain extent. In order to analyze the expression level of GmABCA9 in different soybean tissues, we extracted the RNA of Heinong 44 soybean leaves, roots, stems, seeds and fruit pods 30 days after flowering 30 days after sowing, and detected the expression level of GmABCA9 in different soybean tissues by real-time quantitative ...

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Abstract

The invention discloses a soybean lipid transporter gene GmABCA9 and application thereof. The specific expression of the soybean GmABCA9 gene in soybean seeds and the characteristics of endoplasmic reticulum localization are clarified, and the expression of the gene regulates the development of arabidopsis seeds, and affects the fatty acid content of the seeds, indicating that the gene is involvedin a lipid transport system of endoplasmic reticulum and then regulates the synthesis of seed grease.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a soybean lipid transporter gene and its application. Background technique [0002] Soybean is an important oil crop in my country and the main source of human high-quality protein. It is not only one of the main crops in my country, but also the agricultural product with the largest import volume in my country. In recent years, the oil production of domestic soybean crops has been unable to meet the increasing demand for vegetable oil, and soybean oil in the domestic market is dominated by imported oil. Therefore, it is imminent to cultivate domestic high-oil soybean varieties and increase domestic soybean oil production. [0003] In order to breed high-yield and high-oil soybean varieties, researchers have used genetic engineering to regulate fatty acid synthases and key transcription factors in the lipid metabolism pathway, but the increase in lipid content is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/10A01H6/54A01H6/20
CPCC12N15/8247C07K14/415
Inventor 王邦俊韩二琴马义峰韩丽李健春李英双刘燕王雪宁黄伟鹏
Owner SOUTHWEST UNIVERSITY
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