A soybean lipid transporter gene gmabca9 and its application

A soybean, gene technology, applied in the field of genetic engineering

Active Publication Date: 2022-04-26
SOUTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the transport system of oil between chloroplast and endoplasmic reticulum, and there is no report in soybean

Method used

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  • A soybean lipid transporter gene gmabca9 and its application
  • A soybean lipid transporter gene gmabca9 and its application
  • A soybean lipid transporter gene gmabca9 and its application

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] Cloning of GmABCA9 gene

[0030] Using the RNA of soybean Heinong 44 variety as a template, after synthesizing cDNA, it was amplified by PCR, and the amplification primer pair was:

[0031] 5'-TGCTCTAGAATGGCAACCACTCTCACC-3'

[0032] 5'-TGCTCTAGACCACTGAAAAGCTACTGAACT-3',

[0033] A gene sequence of 2904 bp was obtained, as shown in SEQ ID No. 1, and named soybean GmABCA9 gene; a total of 967 amino acids were encoded, as shown in SEQ ID No. 2.

[0034] Expression analysis of GmABCA9 in soybean tissue

[0035] The spatiotemporal expression of genes provides an important basis for the verification of the function of the target gene to a certain extent. In order to analyze the expression level of GmABCA9 in different soybean tissues, we extracted RNA from leaves, roots, stems, seeds and pods of Heinong 44 soybean 30 days after sowing, and detected the expression of GmABCA9 in different soybean tissues by real-time quantitative PCR. expression level (such as figure 1 ). ...

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Abstract

The invention discloses a soybean lipid transport protein gene GmABCA9 and its application. The invention clarifies the specific expression and endoplasmic reticulum positioning characteristics of the soybean GmABCA9 gene in soybean seeds. The expression of the gene regulates the Development, affecting the fatty acid content of seeds, indicating that the gene is involved in the lipid transport system of the endoplasmic reticulum, and then regulates the synthesis of seed oil.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a soybean lipid transporter gene and application thereof. Background technique [0002] Soybean is an important oil crop in my country and the main source of high-quality protein for human beings. It is not only one of the main crops in my country, but also the agricultural product with the largest import volume in my country. In recent years, the oil production of domestic soybean crops has been unable to meet the increasing demand for vegetable oil, and the soybean oil in the domestic market is dominated by imported oil. Therefore, it is urgent to cultivate domestic high-oil soybean varieties and increase domestic soybean oil production. [0003] In order to cultivate high-yield and high-oil soybean varieties, researchers have used genetic engineering to regulate fatty acid synthase and key transcription factors in the oil metabolism pathway, but the increase in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/10A01H6/54A01H6/20
CPCC12N15/8247C07K14/415
Inventor 王邦俊韩二琴马义峰韩丽李健春李英双刘燕王雪宁黄伟鹏
Owner SOUTHWEST UNIV
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