Kit for simultaneously detecting thalassemia mutant type and deletion type and application thereof

Abstract

The invention discloses a kit for simultaneously detecting thalassemia mutant type and deletion type and application thereof, wherein the kit includes a plurality of amplimer groups for amplifying thalassemia genes; the amplimer groups include primers designed by aiming at alpha globin genes and beta globin gene, and mutant type detecting primers aiming at HBA1 genes, HBA2 genes and HBB genes, anddeletion type detection primers aiming at alpha thalassemia genes -alpha 3.7, -alpha4.2,--SEA,--THAI and beta deletion type SEA-HPFH, sinotype and Taiwantype. Through adopting the kit, the thalassemia mutant type and deletion type can be simultaneously detected, particularly, seven common deletion types and more than 300 mutant types can be simultaneously detected, and novel mutant types can be found out; furthermore, the cost is low, and the kit is much superior to the conventional thalassemia detection kit.

Description

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AI Technical Summary

Benefits of technology

This patented method allows for efficient amplification or identification of genes involved with sickle cell disease (SCD) through use of specific primers that are designed specifically against certain DNA sequences called beta globin proteins found on chromosomally normal red blood cells. These methods have been developed over several decades ago but they still require expensive equipment such as specialized instruments like flow cytometry machines.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the efficiency and effectiveness of early cancer identification tests used today. Current methodologies involve analyzing specific regions called alpha globin loci associated with certain diseases such as sickness syndrome, beta globulism, abnormal liver function, congenital hypothyroid disorder, lymphatic malaria, necrocytosis fugaxia, autochondrial recessed siderosis, fetal hyperchromasia, X-linked hypoplasmosis syndroma, tetanocyte anomalous ghost cell leukodystrophy, sporadic adhesion polyneuropathies, bovine hereditary dyshidase deficiency, porcini ceroids, pigment darkening skin lesions, rickler's syndrome and other similar conditions.

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