A liquid chromatography detection method for chlorphenesin in toy materials

A technology of chlorphenesin and detection method is applied in the field of analytical chemistry to achieve the effect of improving the quality level of toys, good reproducibility and easy operation

Active Publication Date: 2022-04-05
HANGZHOU VOCATIONAL & TECHN COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the problem that there is no detection method for the preservative chlorphenesin in liquid or viscous toys at home and abroad, the present invention provides a liquid chromatography detection method for chlorphenesin in toy materials, which has high accuracy and reproducibility good sex

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  • A liquid chromatography detection method for chlorphenesin in toy materials
  • A liquid chromatography detection method for chlorphenesin in toy materials

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Embodiment 1

[0024] Instruments and reagents:

[0025] Waters H-class ultra-high performance liquid chromatography (U.S. Waters Company), is equipped with diode array detector (DAD) and autosampler; Ultrasonic cleaner (Branson Company), 1 / 10,000 electronic analytical balance (Beijing Sartorius Co., Ltd.), Milli-Q Integral water purifier (French Millipore Company), chlorphenesin (purity 99.5%), methanol (chromatographically pure, Fisher Company); ultrapure water (18.2 MΩ·cm).

[0026] A liquid chromatography detection method for chlorphenesin in toy materials, comprising the following steps:

[0027] (1) Prepare standard solution and fit standard curve equation

[0028] Weigh 0.05g (accurate to 0.0001g) of chlorphenesin, add methanol-water (50:50, v / v), ultrasonically dissolve and transfer to a 50mL volumetric flask, dilute to the mark with methanol-water solution, shake Evenly, it was made into a standard stock solution with a mass concentration of 1000mg / L;

[0029] Pipette 5.0mL of 10...

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Abstract

A high-performance liquid chromatography detection method for chlorphenesin in toy materials, first prepare standard solutions with mass concentrations of 1, 5, 10, 50, 100, 250 and 500 series concentrations; use methanol-water as the mobile phase Gradient elution, using a C-18 column as a chromatographic column with a photodiode array detector, at a detection wavelength of 280nm, a flow rate of 0.2mL / min, a column temperature of 30°C, and an injection volume of 1μL, the high performance liquid chromatography was used for determination in sequence, and the peak The area and concentration were linearly regressed to obtain the regression equation of chlorphenesin; then the sample was weighed, and the extract was added, vortex shaken, ultrasonically extracted, and filter membrane filtered to obtain the sample solution to be tested according to the above method. The obtained peak area is substituted into the regression equation of step (1) to obtain the mass concentration of chlorphenesin in the sample. The method of the invention has the advantages of high accuracy and good reproducibility.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a liquid chromatography detection method for chlorphenesin in toy materials. Background technique [0002] Liquid or viscous toys, such as modeling clay, finger paint, etc., are a type of toy specially designed for children, and are used for modeling or painting by dipping hands or fingers. Children have poor self-control and weak awareness of hygiene. When children use and play with such toys, if they accidentally touch their mouths with their fingers, the toxic and harmful substances in these toys will enter their bodies, which is harmful to children's physical and mental health. cause serious harm. [0003] Chlorphenesin is white to off-white crystalline powder. It is a broad-spectrum antibacterial agent and preservative. It has good antibacterial and bactericidal effects on E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, mold and other microorganisms. At present,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 陈德文季倩祎余妙琴杨浩
Owner HANGZHOU VOCATIONAL & TECHN COLLEGE
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